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AB246889

Anti-PRKRIR antibody

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(1 Publication)

Rabbit Polyclonal PRKRIR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human THAP12 aa 50-250.

View Alternative Names

DAP4, P52RIPK, PRKRIR, THAP0, THAP12, 52 kDa repressor of the inhibitor of the protein kinase, p52rIPK, 58 kDa interferon-induced protein kinase-interacting protein, Death-associated protein 4, THAP domain-containing protein 0, THAP domain-containing protein 12, p58IPK-interacting protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRKRIR antibody (AB246889)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRKRIR antibody (AB246889)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PRKRIR (green) using ab246889 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKRIR antibody (AB246889)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKRIR antibody (AB246889)

Paraffin-embedded human rectum tissue stained for PRKRIR using ab246889 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-PRKRIR antibody (AB246889)
  • WB

Unknown

Western blot - Anti-PRKRIR antibody (AB246889)

All lanes:

Western blot - Anti-PRKRIR antibody (ab246889) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate

Predicted band size: 88 kDa

false

Western blot - Anti-PRKRIR antibody (AB246889)
  • WB

Unknown

Western blot - Anti-PRKRIR antibody (AB246889)

All lanes:

Western blot - Anti-PRKRIR antibody (ab246889) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II whole cell lysate

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human THAP12 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

O43422

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mg of lysate", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKRIR also known as PRK receptor-interacting protein or P58IPK plays a role mechanically as an important component of cellular stress response mechanisms. It acts mainly as an inhibitor of eIF2α phosphorylation which is key to controlling protein synthesis during stress conditions. PRKRIR has an approximate molecular mass of 58 kDa. Researchers have found its expression in various tissues including heart liver and brain. The protein forms a part of cellular response machinery to modulate how cells react to stressors like viral infections or endoplasmic reticulum stress.
Biological function summary

The function of PRKRIR centers on managing cellular stress responses and mitigating apoptosis. It interacts with and regulates the activity of PKR (protein kinase R) a part of the integrated stress response (ISR). As part of its regulatory duties PRKRIR ensures that cells do not overreact to stress signals that might otherwise lead to unnecessary apoptosis. PRKRIR participates as a chaperone-like protein interacting within a complex that ensures the appropriate folding and function of several target proteins engaged in stress adaption pathways.

Pathways

PRKRIR engages in the integrated stress response (ISR) and the unfolded protein response (UPR) pathways. Within these pathways PRKRIR primarily functions to help control the phosphorylation of eIF2α a process heavily regulated under stress conditions to minimize protein synthesis and optimize stress adaptation. It interacts closely with other proteins like PKR and BiP (Binding Immunoglobulin Protein) to modulate these cellular defense mechanisms. By regulating phosphorylation events PRKRIR helps cells to balance survival and apoptotic signals in response to stressful stimuli.

PRKRIR has a significant role in conditions such as neurodegenerative diseases and cancer. In neurodegenerative disorders its role in modulating the ISR could influence neuronal cell survival where dysregulated stress response pathways contribute to disease progression. Cancer research suggests that PRKRIR might be involved in tumor progression where its regulation of protein synthesis can affect cell proliferation rates. Connections to proteins like PKR and eIF2α indicate that altering PRKRIR function can have downstream effects on cellular responses central to disease pathology. Understanding its role offers potential therapeutic interest in designing drugs that modulate stress responses in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR-inhibitory function of DNAJC3, resulting in restoration of kinase activity and suppression of cell growth.
See full target information THAP12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101758 PubMed39368479

2024

CDK12 loss drives prostate cancer progression, transcription-replication conflicts, and synthetic lethality with paralog CDK13.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Ching-Yi Tien,Jie Luo,Yu Chang,Yuping Zhang,Yunhui Cheng,Xiaoju Wang,Jianzhang Yang,Rahul Mannan,Somnath Mahapatra,Palak Shah,Xiao-Ming Wang,Abigail J Todd,Sanjana Eyunni,Caleb Cheng,Ryan J Rebernick,Lanbo Xiao,Yi Bao,James Neiswender,Rachel Brough,Stephen J Pettitt,Xuhong Cao,Stephanie J Miner,Licheng Zhou,Yi-Mi Wu,Estefania Labanca,Yuzhuo Wang,Abhijit Parolia,Marcin Cieslik,Dan R Robinson,Zhen Wang,Felix Y Feng,Jonathan Chou,Christopher J Lord,Ke Ding,Arul M Chinnaiyan
View all publications
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Product promise

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