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AB154739

Anti-PRKX antibody

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(1 Publication)

Rabbit Polyclonal PRKX antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PRKX aa 1-350.

View Alternative Names

PKX1, PRKX, cAMP-dependent protein kinase catalytic subunit PRKX, PrKX, Protein kinase X, Protein kinase X-linked, Serine/threonine-protein kinase PRKX, Protein kinase PKX1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKX antibody (AB154739)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKX antibody (AB154739)

Immunohistochemical analysis of paraffin-embedded Human H3347 xenograft tissue labeling PRKX with ab154739 at 1/500 dilution.

Western blot - Anti-PRKX antibody (AB154739)
  • WB

Unknown

Western blot - Anti-PRKX antibody (AB154739)

10% SDS PAGE

All lanes:

Western blot - Anti-PRKX antibody (ab154739) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 41 kDa

false

Western blot - Anti-PRKX antibody (AB154739)
  • WB

Unknown

Western blot - Anti-PRKX antibody (AB154739)

10% SDS PAGE

All lanes:

Western blot - Anti-PRKX antibody (ab154739) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PRKX aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P51817

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKX also known as Protein Kinase X is a serine/threonine-protein kinase with a molecular mass of about 37 kDa. This protein is part of the cAMP-dependent protein kinase family. Scientists observe PRKX expression mainly in the kidney heart and central nervous system. It helps regulate multiple functions by phosphorylating various substrates which are important to cell signaling and communication. The presence of the protein in specific tissues suggests a significant role in development and organ function.
Biological function summary

The protein functions as an important regulator in cellular signaling processes. PRKX interacts with other proteins as a monomer rather than as part of a larger complex. Its activity influences cellular growth differentiation and gene expression. The kinase activity shows high involvement in developmental pathways across numerous cell types. Additionally its interactions affect cellular features like polarization and motility which are important for normal physiological processes.

Pathways

PRKX serves important roles in the PKA signaling pathway and the renal development pathway. PKA signaling involves cAMP as a second messenger and PRKX acts downstream as a phosphorylating agent for various targets within the cell. In the renal development pathway PRKX collaborates with other protein kinases like PRKY which shares a similar sequence and function reflecting redundancy and specialization in developmental roles in kidneys.

The role of PRKX becomes significant in congenital kidney disorders particularly certain types of cystic kidney diseases. The protein's influence on renal development highlights its connection to these conditions. Additionally anomalies in PRKX expression or function can link to central nervous system disorders influencing neurological development and function. Within these contexts PRKX often interacts with related protein networks involving kinases and substrates important for maintaining cellular health and functionality.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.
See full target information PRKX
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 10:579 PubMed35722357

2022

Long non-coding RNA (FALEC) promotes malignant behaviors of gastric cancer cells by regulating miR-203b/PIM3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Dong,Mancheng Gong,Jianjun Xiao,Huifen Li,Muyou Tian,Senming Wang
View all publications
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Product promise

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