AB109389

Anti-PRKX antibody [EPR4840]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PRKX antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PKX1, PRKX, cAMP-dependent protein kinase catalytic subunit PRKX, PrKX, Protein kinase X, Protein kinase X-linked, Serine/threonine-protein kinase PRKX, Protein kinase PKX1

1 Images

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Western blot - Anti-PRKX antibody [EPR4840] (AB109389)

All lanes:

Western blot - Anti-PRKX antibody [EPR4840] (ab109389) at 1/1000 dilution

Lane 1:

A549 cell lysates at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Lane 4:

Human fetal kidney lysates at 10 µg

Lane 5:

Human fetal brain lysates at 10 µg

Predicted band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4840

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRKX also known as Protein Kinase X is a serine/threonine-protein kinase with a molecular mass of about 37 kDa. This protein is part of the cAMP-dependent protein kinase family. Scientists observe PRKX expression mainly in the kidney heart and central nervous system. It helps regulate multiple functions by phosphorylating various substrates which are important to cell signaling and communication. The presence of the protein in specific tissues suggests a significant role in development and organ function.

Biological function summary

The protein functions as an important regulator in cellular signaling processes. PRKX interacts with other proteins as a monomer rather than as part of a larger complex. Its activity influences cellular growth differentiation and gene expression. The kinase activity shows high involvement in developmental pathways across numerous cell types. Additionally its interactions affect cellular features like polarization and motility which are important for normal physiological processes.

Pathways

PRKX serves important roles in the PKA signaling pathway and the renal development pathway. PKA signaling involves cAMP as a second messenger and PRKX acts downstream as a phosphorylating agent for various targets within the cell. In the renal development pathway PRKX collaborates with other protein kinases like PRKY which shares a similar sequence and function reflecting redundancy and specialization in developmental roles in kidneys.

The role of PRKX becomes significant in congenital kidney disorders particularly certain types of cystic kidney diseases. The protein's influence on renal development highlights its connection to these conditions. Additionally anomalies in PRKX expression or function can link to central nervous system disorders influencing neurological development and function. Within these contexts PRKX often interacts with related protein networks involving kinases and substrates important for maintaining cellular health and functionality.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.

See full target information PRKX

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 41:43-56 PubMed30365128

2018

PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Tang,Wenjin Liang,Yong Liao,Zeming Li,Yan Wang,Chao Yan

The Journal of biological chemistry 289:24226-37 PubMed25028512

2014

Transforming growth factor (TGF)-β-activated kinase 1 (TAK1) activation requires phosphorylation of serine 412 by protein kinase A catalytic subunit α (PKACα) and X-linked protein kinase (PRKX).

Applications

Species

Unspecified reactive species

Chuan Ouyang,Li Nie,Meidi Gu,Ailing Wu,Xu Han,Xiaojian Wang,Jianzhong Shao,Zongping Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRKX antibody [EPR4840]

Western blot - Anti-PRKX antibody [EPR4840] (AB109389)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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