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Rabbit Recombinant Monoclonal PRMT1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Tested
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/80

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:10749851, PubMed:16879614, PubMed:26876602). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).

Alternative names

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Rabbit Recombinant Monoclonal PRMT1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18344

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

PRMT1 participates in several essential cellular functions including signal transduction gene expression and RNA processing. It methylates histones and non-histone proteins influencing chromatin structure and function. PRMT1 does not normally function as part of a complex but it interacts individually with different substrates to exert its effects. PRMT1 activity is essential in regulating transcriptional activation and repression highlighting its significant role in cellular regulation.

Activity summary

PRMT1 also known as protein arginine methyltransferase 1 is an important enzyme for post-translational modification. With a molecular mass of approximately 41 kDa PRMT1 is involved in the methylation of arginine residues on target proteins. This enzyme is ubiquitously expressed across various tissues indicating its widespread importance in cellular functions. PRMT1 functions in both the nucleus and cytoplasm impacting diverse cellular processes through its enzymatic activity.

Pathways

PRMT1 plays a central role in the regulation of the glucocorticoid receptor signaling and p53 pathways. In glucocorticoid receptor signaling PRMT1 modifies transcription factors affecting their ability to bind DNA and regulate gene expression. In the p53 pathway PRMT1 methylates p53 itself impacting cell cycle arrest and apoptotic functions. These pathways demonstrate PRMT1’s interaction with other proteins like the p53 protein highlighting its integrative role in essential cellular processes.

Associated diseases and disorders

PRMT1 is implicated in cancer and cardiovascular diseases. Aberrant expression or activity of PRMT1 associates with tumor progression and poor prognosis in cancers making it a target for therapeutic intervention. Additionally PRMT1 contributes to the pathogenesis of cardiovascular diseases by influencing the expression of genes linked to cardiac hypertrophy. PRMT1 interacts with the p53 protein impacting cancer development while also being connected through its regulatory function on p53-associated genes in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (ab190892)

    PRMT1 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab190892 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab190892 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate 10ug (Input).

    Lane 2: ab190892 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190892 in HEK293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (AB190892)

    Developed using the ECL technique.

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 30s

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PRMT1 with ab190892 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab190892 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 dilution.
    -ve control 2: anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892) at 1/1000 dilution

    All lanes: A549 (Human lung carcinoma cell line) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on Human breast cancer tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cell line) cell lysate at 10 µg

    Lane 2: RAW264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining and weak cytoplasmic staining on rat colon tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    It has been shown that in living cells PRMT1 shuttles between the nucleus and the cytoplasm depending on the methylation status of substrate proteins. Genes to Cells (2009) 14, 309–317.

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892), expandable thumbnail

    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab181602 was used as a GAPDH loading control.

    We recommend using a higher sensitive ECL substrate to increase the band intensity. ab92299 could be an alternative for getting stronger signal.

    Lanes 1 - 6: Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892) at 1/1000 dilution

    Lanes 7 - 9: Western blot - Anti-PRMT1 antibody [EPR3292] (AB92299) at 1/2000 dilution

    Lanes 1, 4 and 7: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 2, 5 and 8: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 3, 6 and 9: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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