Anti-PRMT1 antibody [EPR18344]
- RabMAb
- Recombinant
- 20ul selling size
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(15 Publications)
Rabbit Recombinant Monoclonal PRMT1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 15 publications.
View Alternative Names
HMT2, HRMT1L2, IR1B4, PRMT1, Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (AB190892)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (AB190892)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PRMT1 with ab190892 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab190892 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 dilution.
-ve control 2 : anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (AB190892)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on Human breast cancer tissue is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (AB190892)
PRMT1 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab190892 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab190892 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HEK-293 whole cell lysate 10ug (Input).
Lane 2 : ab190892 IP in HEK-293 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190892 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 30 seconds.
All lanes:
Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (ab190892)
Predicted band size: 42 kDa
Observed band size: 42 kDa
true
Exposure time: 30s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (AB190892)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
It has been shown that in living cells PRMT1 shuttles between the nucleus and the cytoplasm depending on the methylation status of substrate proteins. Genes to Cells (2009) 14, 309–317.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (AB190892)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining and weak cytoplasmic staining on rat colon tissue is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
- WB
Supplier Data
Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab181602 was used as a GAPDH loading control.
We recommend using a higher sensitive ECL substrate to increase the band intensity. ab92299 could be an alternative for getting stronger signal.
Lanes 1 - 6:
Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/1000 dilution
Lanes 7 - 9:
Western blot - Anti-PRMT1 antibody [EPR3292] (<a href='/en-us/products/primary-antibodies/prmt1-antibody-epr3292-ab92299'>ab92299</a>) at 1/2000 dilution
Lanes 1, 4 and 7:
Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 2, 5 and 8:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 3, 6 and 9:
HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
Exposure time: 180s
- WB
Unknown
Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/1000 dilution
All lanes:
A549 (Human lung carcinoma cell line) cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
Exposure time: 3min
- WB
Unknown
Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/5000 dilution
Lane 1:
HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2:
HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg
Lane 3:
NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
Exposure time: 3min
- WB
Unknown
Western blot - Anti-PRMT1 antibody [EPR18344] (AB190892)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/1000 dilution
Lane 1:
C6 (Rat glial tumor cell line) cell lysate at 10 µg
Lane 2:
RAW264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg
Lane 3:
PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
Exposure time: 3min
Related conjugates and formulations (8)
-
Anti-PRMT1 antibody [EPR18344] - BSA and Azide free
-
660 APC
APC Anti-PRMT1 antibody [EPR18344]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-PRMT1 antibody [EPR18344]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-PRMT1 antibody [EPR18344]
-
578 PE
PE Anti-PRMT1 antibody [EPR18344]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-PRMT1 antibody [EPR18344]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-PRMT1 antibody [EPR18344]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-PRMT1 antibody [EPR18344]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PRMT1 participates in several essential cellular functions including signal transduction gene expression and RNA processing. It methylates histones and non-histone proteins influencing chromatin structure and function. PRMT1 does not normally function as part of a complex but it interacts individually with different substrates to exert its effects. PRMT1 activity is essential in regulating transcriptional activation and repression highlighting its significant role in cellular regulation.
Pathways
PRMT1 plays a central role in the regulation of the glucocorticoid receptor signaling and p53 pathways. In glucocorticoid receptor signaling PRMT1 modifies transcription factors affecting their ability to bind DNA and regulate gene expression. In the p53 pathway PRMT1 methylates p53 itself impacting cell cycle arrest and apoptotic functions. These pathways demonstrate PRMT1’s interaction with other proteins like the p53 protein highlighting its integrative role in essential cellular processes.
Product protocols
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Target data
Publications (15)
Recent publications for all applications. Explore the full list and refine your search
Clinical epigenetics 17:129 PubMed40696470
2025
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Communications biology 8:159 PubMed39901028
2025
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Acta biochimica et biophysica Sinica 57:1338-1349 PubMed39659162
2024
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Cell death & disease 15:814 PubMed39528487
2024
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Communications biology 7:753 PubMed38902349
2024
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Nature communications 15:4790 PubMed38839752
2024
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Journal of clinical laboratory analysis 38:e25030 PubMed38525916
2024
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Genes & diseases 10:2622-2638 PubMed37554218
2023
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Nature communications 14:1011 PubMed36823188
2023
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International journal of molecular sciences 22: PubMed34830164
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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