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AB7027

Anti-PRMT1 antibody [MAT-B12]

3

(1 Review)

|

(18 Publications)

Mouse Monoclonal PRMT1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Protein within Human PRMT1.

View Alternative Names

HMT2, HRMT1L2, IR1B4, PRMT1, Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4

2 Images
Flow Cytometry - Anti-PRMT1 antibody [MAT-B12] (AB7027)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-PRMT1 antibody [MAT-B12] (AB7027)

Overlay histogram showing HeLa cells stained with ab7027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7027, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [MAT-B12] (AB7027)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [MAT-B12] (AB7027)

Immunofluorecent analysis of MCF-7 cells labelling PRMT1 with ab7027

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MAT-B12

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Protein within Human PRMT1. The exact immunogen used to generate this antibody is proprietary information.

Q99873

Specificity

This Mab reacts with human arginine N-Methyl Transferase

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRMT1 also known as protein arginine methyltransferase 1 is an important enzyme for post-translational modification. With a molecular mass of approximately 41 kDa PRMT1 is involved in the methylation of arginine residues on target proteins. This enzyme is ubiquitously expressed across various tissues indicating its widespread importance in cellular functions. PRMT1 functions in both the nucleus and cytoplasm impacting diverse cellular processes through its enzymatic activity.
Biological function summary

PRMT1 participates in several essential cellular functions including signal transduction gene expression and RNA processing. It methylates histones and non-histone proteins influencing chromatin structure and function. PRMT1 does not normally function as part of a complex but it interacts individually with different substrates to exert its effects. PRMT1 activity is essential in regulating transcriptional activation and repression highlighting its significant role in cellular regulation.

Pathways

PRMT1 plays a central role in the regulation of the glucocorticoid receptor signaling and p53 pathways. In glucocorticoid receptor signaling PRMT1 modifies transcription factors affecting their ability to bind DNA and regulate gene expression. In the p53 pathway PRMT1 methylates p53 itself impacting cell cycle arrest and apoptotic functions. These pathways demonstrate PRMT1’s interaction with other proteins like the p53 protein highlighting its integrative role in essential cellular processes.

PRMT1 is implicated in cancer and cardiovascular diseases. Aberrant expression or activity of PRMT1 associates with tumor progression and poor prognosis in cancers making it a target for therapeutic intervention. Additionally PRMT1 contributes to the pathogenesis of cardiovascular diseases by influencing the expression of genes linked to cardiac hypertrophy. PRMT1 interacts with the p53 protein impacting cancer development while also being connected through its regulatory function on p53-associated genes in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2 (PubMed : 10749851, PubMed : 15741314, PubMed : 16879614, PubMed : 18951090, PubMed : 22095282, PubMed : 25284789, PubMed : 26575292, PubMed : 26876602, PubMed : 27642082, PubMed : 30765518, PubMed : 31257072, PubMed : 38006878). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed : 26575292). Methylates MRE11 and TP53BP1, promoting the DNA damage response (PubMed : 15741314, PubMed : 16294045, PubMed : 29651020). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity (PubMed : 18951090). Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed : 25284789). Methylates MAP3K5/ASK1 at 'Arg-78' and 'Arg-80' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK (PubMed : 22095282). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed : 25284789). Plays a role in regulating alternative splicing in the heart (By similarity). Methylates NPRL2 at 'Arg-78' leading to inhibition of its GTPase activator activity and then the GATOR1 complex and consequently inducing timely mTORC1 activation under methionine-sufficient conditions (PubMed : 38006878).
See full target information PRMT1
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 26:1656-1671 PubMed35092120

2022

CTNNAL1 participates in the regulation of mucus overproduction in HDM-induced asthma mouse model through the YAP-ROCK2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wu,Wang Jiang,Caixia Liu,Lexin Liu,Furong Li,Xiaodi Ma,Lang Pan,Chi Liu,Xiangping Qu,Huijun Liu,Xiaoqun Qin,Yang Xiang

The Journal of clinical investigation 132: PubMed35040433

2022

Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Yi Sung,Yi-Hsin Lin,Qiongman Kong,Pali D Shah,Joan Glick Bieler,Scott Palmer,Kent J Weinhold,Hong-Ru Chang,Hailiang Huang,Robin K Avery,Jonathan Schneck,Yen-Ling Chiu

PloS one 12:e0185042 PubMed28934323

2017

Identification, chromosomal arrangements and expression analyses of the evolutionarily conserved prmt1 gene in chicken in comparison with its vertebrate paralogue prmt8.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chun Wang,Chien-Wen Wang,Wen-Chang Lin,Yun-Jung Tsai,Chien-Ping Chang,Yu-Jen Lee,Min-Jon Lin,Chuan Li

Cancer research 77:4613-4625 PubMed28655788

2017

PRMT1-Mediated Translation Regulation Is a Crucial Vulnerability of Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jessie Hao-Ru Hsu,Benjamin Hubbell-Engler,Guillaume Adelmant,Jialiang Huang,Cailin E Joyce,Francisca Vazquez,Barbara A Weir,Philip Montgomery,Aviad Tsherniak,Andrew O Giacomelli,Jennifer A Perry,Jennifer Trowbridge,Yuko Fujiwara,Glenn S Cowley,Huafeng Xie,Woojin Kim,Carl D Novina,William C Hahn,Jarrod A Marto,Stuart H Orkin

PloS one 8:e55483 PubMed23408989

2013

Protein arginine methylation is more prone to inhibition by S-adenosylhomocysteine than DNA methylation in vascular endothelial cells.

Applications

WB

Species

Human

Ruben Esse,Monica S Rocha,Madalena Barroso,Cristina Florindo,Tom Teerlink,Robert M Kok,Yvo M Smulders,Isabel Rivera,Paula Leandro,Pieter Koolwijk,Rita Castro,Henk J Blom,Isabel Tavares de Almeida

ACS chemical biology 6:1127-35 PubMed21838253

2011

Activity-based protein profiling of protein arginine methyltransferase 1.

Applications

Unspecified application

Species

Human

Obiamaka Obianyo,Corey P Causey,Justin E Jones,Paul R Thompson

Biochemistry 50:3997-4010 PubMed21466234

2011

Autodeimination of protein arginine deiminase 4 alters protein-protein interactions but not activity.

Applications

WB

Species

Human

Jessica L Slack,Larry E Jones,Monica M Bhatia,Paul R Thompson

Human molecular genetics 19:1314-23 PubMed20064924

2010

Arginines of the RGG box regulate FMRP association with polyribosomes and mRNA.

Applications

WB

Species

Human

Ernest Blackwell,Xing Zhang,Stephanie Ceman

Journal of virology 81:13209-17 PubMed17686851

2007

Critical role for arginine methylation in adenovirus-infected cells.

Applications

Unspecified application

Species

Unspecified reactive species

Demetris C Iacovides,Clodagh C O'Shea,Juan Oses-Prieto,Alma Burlingame,Frank McCormick

Proceedings of the National Academy of Sciences of 103:13351-6 PubMed16938873

2006

Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene.

Applications

ChIP

Species

Mouse

Selma El Messaoudi,Eric Fabbrizio,Carmen Rodriguez,Paul Chuchana,Lucas Fauquier,Donghang Cheng,Charles Theillet,Laurence Vandel,Mark T Bedford,Claude Sardet
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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