JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122374

Anti-PRMT10 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal PRMT10 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRMT9 aa 200-350.

View Alternative Names

PRMT10, PRMT9, Protein arginine N-methyltransferase 9, Protein arginine N-methyltransferase 10

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRMT10 antibody (AB122374)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRMT10 antibody (AB122374)

Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab122374 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT10 antibody (AB122374)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT10 antibody (AB122374)

ab122374, at 1/200 dilution, staining PRMT10 in paraffin embedded Human tonsil tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PRMT9 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q6P2P2

style
left-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB109451

Anti-PRMT5 antibody [EPR5772]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody [EPR5772] (AB109451)

  • 5
  • 2
Primary Antibodies

AB190892

Anti-PRMT1 antibody [EPR18344]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (AB190892)

  • 0
  • 0
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRMT10 also known as Protein Arginine Methyltransferase 10 participates in the methylation of arginine residues in target proteins. This enzyme functions mainly in cellular locations such as the nucleus and the cytoplasm. Weighing around 64 kDa PRMT10 modifies histones through methylation playing a critical role in transcriptional regulation and other chromatin-related processes. Expression of PRMT10 can be seen across many tissues with higher levels found in actively dividing cells due to its role in cell cycle regulation.
Biological function summary

PRMT10 affects chromatin structure and gene expression by methylating histones and other substrates. This protein often associates with complexes involved in transcriptional repression or activation. It targets histones like H3 and H4 influencing the recruitment of transcriptional machinery and chromatin remodelers. Through its enzymatic activity PRMT10 impacts processes like gene silencing DNA repair and signal transduction.

Pathways

The enzymatic activity of PRMT10 integrates with several cellular pathways. It prominently operates in the regulation of the Wnt signaling pathway and the cell cycle control pathway. PRMT10 works alongside related proteins such as β-catenin in Wnt signaling modulating gene expression patterns critical for cell growth and differentiation. Furthermore its interaction with cyclin-dependent kinases (CDKs) highlights its involvement in cell cycle checkpoints.

PRMT10 has been linked to cancer and neurodegenerative diseases. Aberrant expression and function of PRMT10 contribute to tumorigenesis by influencing oncogene and tumor suppressor gene expression. In cancer PRMT10 interacts with proteins like Myc enhancing its transcriptional activity which promotes cell proliferation. In neurodegenerative disorders altered PRMT10 activity affects neuronal gene expression highlighting its potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of SF3B2. Involved in the regulation of alternative splicing of pre-mRNA (PubMed : 25737013, PubMed : 25979344).
See full target information PRMT9
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:625 PubMed37715221

2023

Arginine methylation of HSPA8 by PRMT9 inhibits ferroptosis to accelerate hepatitis B virus-associated hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wensheng Deng,Jiaoyu Ai,Wanlin Zhang,Zhenyu Zhou,Muqi Li,Likun Yan,Lidong Zhang,Zongjing Huang,Ziyi Wu,Junhua Ai,Hai Jiang

The Journal of clinical investigation 133: PubMed37463447

2023

RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Launay,Montserrat Ruiz,Laura Planas-Serra,Edgard Verdura,Agustí Rodríguez-Palmero,Agatha Schlüter,Leire Goicoechea,Cristina Guilera,Josefina Casas,Felix Campelo,Emmanuelle Jouanguy,Jean-Laurent Casanova,Odile Boespflug-Tanguy,Maria Vazquez Cancela,Luis González Gutiérrez-Solana,Carlos Casasnovas,Estela Area-Gomez,Aurora Pujol
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com