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AB154154

Anti-PRMT2/HMT1 antibody

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(6 Publications)

Rabbit Polyclonal PRMT2/HMT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRMT2 aa 1-350.

View Alternative Names

HMT1, HRMT1L1, PRMT2, Protein arginine N-methyltransferase 2, Histone-arginine N-methyltransferase PRMT2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRMT2/HMT1 antibody (AB154154)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRMT2/HMT1 antibody (AB154154)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PRMT2/HMT1 with ab154154 at 1/200 dilution. The image in the lower panel was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT2/HMT1 antibody (AB154154)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT2/HMT1 antibody (AB154154)

Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling PRMT2/HMT1 with ab154154 at 1/100 dilution.

Western blot - Anti-PRMT2/HMT1 antibody (AB154154)
  • WB

Supplier Data

Western blot - Anti-PRMT2/HMT1 antibody (AB154154)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PRMT2/HMT1 antibody (ab154154) at 1/1000 dilution

Lane 1:

Neuro2a whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PRMT2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P55345

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Arginine Methyltransferase 2 (PRMT2) also known as HMT1 is a member of the protein arginine methyltransferase family. This enzyme carries out the transfer of methyl groups to arginine residues on target proteins influencing gene expression. PRMT2 has a molecular mass of about 59 kDa. It is detected widely in human tissues most notably in the liver heart and brain. This distribution indicates its involvement in a broad range of cellular activities.
Biological function summary

PRMT2 regulates various processes through its methylation activity. It functions as a coactivator particularly in the nuclear hormone receptor pathway. This protein is part of complexes that modify chromatin affecting gene transcription regulation. Through methylation of histones and other proteins PRMT2 influences cellular differentiation and proliferation. By modifying these key components PRMT2 controls important physiological functions within cells.

Pathways

PRMT2 interacts closely with nuclear hormone receptor signaling and pathways associated with gene regulation. These connections make it particularly important in the estrogen receptor signaling pathway where PRMT2 acts in conjunction with other proteins like PRMT1. Additionally PRMT2 has roles in influencing the PI3K/AKT pathway an important signaling pathway related to cell survival and metabolism.

PRMT2 exhibits relevant associations with conditions like cancer and neurological disorders. In cancer altered PRMT2 expression can misregulate gene expression through disrupted methylation promoting tumorigenic processes. Its dysregulation also connects to inflammation and degenerative diseases in the nervous system often involving proteins like PRMT1 as collaborators in pathology development. Understanding these links with diseases can guide therapeutic approaches involving PRMT2 modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
See full target information PRMT2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37958610

2023

Resistance to 2-Hydroxy-Flutamide in Prostate Cancer Cells Is Associated with the Downregulation of Phosphatidylcholine Biosynthesis and Epigenetic Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

José María Mora-Rodríguez,Belén G Sánchez,Alba Sebastián-Martín,Alba Díaz-Yuste,Manuel Sánchez-Chapado,Ana María Palacín,Carlos Sánchez-Rodríguez,Alicia Bort,Inés Díaz-Laviada

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22776 PubMed36688817

2023

Loss of aquaporin 5 contributes to the corneal epithelial pathogenesis via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Guohu Di,Kaier Zhang,Ying Bai,Xin Cao,Hui Zhao,Dianqiang Wang,Peng Chen

Cellular and molecular life sciences : CMLS 79:306 PubMed35593921

2022

Type I IFN stimulates IFI16-mediated aromatase expression in adipocytes that promotes E-dependent growth of ER-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Na-Lee Ka,Ga Young Lim,Seung-Su Kim,Sewon Hwang,Juhyeong Han,Yun-Hee Lee,Mi-Ock Lee

British journal of pharmacology 179:141-158 PubMed34599829

2021

Protein arginine methyltransferase 2 (PRMT2) promotes dextran sulfate sodium-induced colitis by inhibiting the SOCS3 promoter via histone H3R8 asymmetric dimethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Li,Xiaohua Pan,Zhengnan Ren,Binbin Li,He Liu,Chengfei Wu,Xiaoliang Dong,Paul de Vos,Li-Long Pan,Jia Sun

Cell reports 32:108172 PubMed32997990

2020

Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ashok Kumar,Yuan Zhong,Amelie Albrecht,Pau Biak Sang,Adrian Maples,Zhenan Liu,Vinesh Vinayachandran,Rohit Reja,Chia-Fang Lee,Ashutosh Kumar,Jiyuan Chen,Jing Xiao,Bongsoo Park,Jianjun Shen,Bin Liu,Maria D Person,Kathleen M Trybus,Kam Y J Zhang,B Franklin Pugh,Kristine E Kamm,Dianna M Milewicz,Xuetong Shen,Prabodh Kapoor

Molecular endocrinology (Baltimore, Md.) 28:1166-85 PubMed24911119

2014

PRMT2 and RORγ expression are associated with breast cancer survival outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Gyu Oh,Peter Bailey,Eloise Dray,Aaron G Smith,Joel Goode,Natalie Eriksson,John W Funder,Peter J Fuller,Evan R Simpson,Wayne D Tilley,Peter J Leedman,Christine L Clarke,Sean Grimmond,Dennis H Dowhan,George E O Muscat
View all publications
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