Rabbit Polyclonal PRMT2/HMT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRMT2 aa 1-350.
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Resistance to 2-Hydroxy-Flutamide in Prostate Cancer Cells Is Associated with the Downregulation of Phosphatidylcholine Biosynthesis and Epigenetic Modifications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé María Mora-Rodríguez,Belén G Sánchez,Alba Sebastián-Martín,Alba Díaz-Yuste,Manuel Sánchez-Chapado,Ana María Palacín,Carlos Sánchez-Rodríguez,Alicia Bort,Inés Díaz-LaviadaPubMed 37958610
- Cellular and molecular life sciences : CMLS 79:3062022Type I IFN stimulates IFI16-mediated aromatase expression in adipocytes that promotes E-dependent growth of ER-positive breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa-Lee Ka,Ga Young Lim,Seung-Su Kim,Sewon Hwang,Juhyeong Han,Yun-Hee Lee,Mi-Ock LeePubMed 35593921
- British journal of pharmacology 179:141-1582021Protein arginine methyltransferase 2 (PRMT2) promotes dextran sulfate sodium-induced colitis by inhibiting the SOCS3 promoter via histone H3R8 asymmetric dimethylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahong Li,Xiaohua Pan,Zhengnan Ren,Binbin Li,He Liu,Chengfei Wu,Xiaoliang Dong,Paul de Vos,Li-Long Pan,Jia SunPubMed 34599829
- Cell reports 32:1081722020Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshok Kumar,Yuan Zhong,Amelie Albrecht,Pau Biak Sang,Adrian Maples,Zhenan Liu,Vinesh Vinayachandran,Rohit Reja,Chia-Fang Lee,Ashutosh Kumar,Jiyuan Chen,Jing Xiao,Bongsoo Park,Jianjun Shen,Bin Liu,Maria D Person,Kathleen M Trybus,Kam Y J Zhang,B Franklin Pugh,Kristine E Kamm,Dianna M Milewicz,Xuetong Shen,Prabodh KapoorPubMed 32997990
- Molecular endocrinology (Baltimore, Md.) 28:1166-852014PRMT2 and RORγ expression are associated with breast cancer survival outcomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae Gyu Oh et. al.PubMed 24911119
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PRMT2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P55345
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
Alternative names
HMT1, HRMT1L1, HRMT1L1, HMT1, PRMT2, Protein arginine N-methyltransferase 2, Histone-arginine N-methyltransferase PRMT2
Recommended products
Rabbit Polyclonal PRMT2/HMT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRMT2 aa 1-350.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PRMT2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P55345
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Arginine Methyltransferase 2 (PRMT2) also known as HMT1 is a member of the protein arginine methyltransferase family. This enzyme carries out the transfer of methyl groups to arginine residues on target proteins influencing gene expression. PRMT2 has a molecular mass of about 59 kDa. It is detected widely in human tissues most notably in the liver heart and brain. This distribution indicates its involvement in a broad range of cellular activities.
- Biological function summary
PRMT2 regulates various processes through its methylation activity. It functions as a coactivator particularly in the nuclear hormone receptor pathway. This protein is part of complexes that modify chromatin affecting gene transcription regulation. Through methylation of histones and other proteins PRMT2 influences cellular differentiation and proliferation. By modifying these key components PRMT2 controls important physiological functions within cells.
- Pathways
PRMT2 interacts closely with nuclear hormone receptor signaling and pathways associated with gene regulation. These connections make it particularly important in the estrogen receptor signaling pathway where PRMT2 acts in conjunction with other proteins like PRMT1. Additionally PRMT2 has roles in influencing the PI3K/AKT pathway an important signaling pathway related to cell survival and metabolism.
- Associated diseases and disorders
PRMT2 exhibits relevant associations with conditions like cancer and neurological disorders. In cancer altered PRMT2 expression can misregulate gene expression through disrupted methylation promoting tumorigenic processes. Its dysregulation also connects to inflammation and degenerative diseases in the nervous system often involving proteins like PRMT1 as collaborators in pathology development. Understanding these links with diseases can guide therapeutic approaches involving PRMT2 modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-PRMT2/HMT1 antibody (ab154154)
10% SDS PAGE
All lanes: Western blot - Anti-PRMT2/HMT1 antibody (ab154154) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 49 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PRMT2/HMT1 antibody (ab154154)
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PRMT2/HMT1 with ab154154 at 1/200 dilution. The image in the lower panel was co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT2/HMT1 antibody (ab154154)
Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling PRMT2/HMT1 with ab154154 at 1/100 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com