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Rabbit Recombinant Monoclonal PRMT3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (AB240322), expandable thumbnail
  • Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (AB240322), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (AB240322), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (Probable). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity (PubMed:33495566).

Alternative names

HRMT1L3, PRMT3, Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3

Recommended products

Rabbit Recombinant Monoclonal PRMT3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13279
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240322 is the carrier-free version of Anti-PRMT3 antibody [EPR13279] ab191562.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Protein arginine methyltransferase 3 (PRMT3) plays an important role in the methylation of arginine residues on various substrates which influences protein-protein interactions signal transduction and gene expression. Also known as HRMT1L3 PRMT3 has an approximate molecular weight of 59 kDa. Researchers find this enzyme mainly in the cytoplasm although it can also localize in the nucleus. Expression of PRMT3 happens across multiple tissues with notable presence in the liver and lungs.

Biological function summary

PRMT3 methylates ribosomal protein S2 an activity that suggests its participation in ribosome assembly and nucleolar functions. It forms a complex with ribosomal proteins and several metabolic enzymes which highlight its involvement in protein synthesis and cellular metabolic regulation. The activity of PRMT3 regulates the biogenesis of ribosomes which are central to protein production in cells.

Pathways

PRMT3 influences various cellular processes like the ribosome biogenesis and the mTOR signaling pathway. Its interaction with the ribosome synthesis pathway aids in the maturation and assembly of ribosomal subunits. In the mTOR pathway PRMT3 relates to S6 kinase and eukaryotic initiation factors affecting cell growth and metabolism through the regulation of protein synthesis.

Associated diseases and disorders

PRMT3 may influence the development of metabolic syndromes and certain cancers. Studies suggest a potential link between PRMT3 dysregulation and the onset of insulin resistance making it significant in the context of type 2 diabetes. Additionally abnormal PRMT3 expression connects with progression of malignancies potentially through interacting with oncogenic proteins and altering signaling pathways that regulate cell cycle and apoptosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

    Immunohistochemical analysis of paraffin-embedded, human cervix carcinoma tissue labeling PRMT3 with Anti-PRMT3 antibody [EPR13279] ab191562 at a 1/100 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRMT3 antibody [EPR13279] ab191562).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322), expandable thumbnail

    Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling PRMT3 using Anti-PRMT3 antibody [EPR13279] ab191562 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRMT3 antibody [EPR13279] ab191562).

    All lanes: Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] (Anti-PRMT3 antibody [EPR13279] ab191562)

    Developed using the ECL technique.

    Predicted band size: 60 kDa

    Observed band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] - BSA and Azide free (ab240322)

    Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling PRMT3 with Anti-PRMT3 antibody [EPR13279] ab191562 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PRMT3 at a 1/50 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRMT3 antibody [EPR13279] ab191562).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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