Mouse Monoclonal PRMT6 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRMT6.
Images
![Flow Cytometry - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (AB277102), expandable thumbnail](https://content.abcam.com/products/images/prmt6-antibody-pcrp-prmt6-2c9-ab277102--flow-cytometry-img95702.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (AB277102)")
![Protein Array - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (AB277102), expandable thumbnail](https://content.abcam.com/products/images/prmt6-antibody-pcrp-prmt6-2c9-ab277102--protein-array-img99554.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (AB277102)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PRMT6. Database link Q96LA8
Reactivity data
Select an application| Flow Cyt | Protein Array | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910, PubMed:30420520). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed:17898714, PubMed:18079182, PubMed:18077460). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed:17898714, PubMed:18077460). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed:19509293). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed:16600869). Methylates HMGA1 (PubMed:16157300, PubMed:16159886). Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity). May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed:17267505). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity). Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed:30420520).
Alternative names
Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6, PRMT6
Recommended products
Mouse Monoclonal PRMT6 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRMT6.
Alternative names
Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6, PRMT6
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PRMT6. Database link Q96LA8
- Clone number
PCRP-PRMT6-2C9
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (ab277102), expandable thumbnail](https://content.abcam.com/products/images/prmt6-antibody-pcrp-prmt6-2c9-ab277102--flow-cytometry-img95702.jpg "Flow Cytometry - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (ab277102)")
Flow Cytometry - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (ab277102)
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PRMT6 using ab277102 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
![Protein Array - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (ab277102), expandable thumbnail](https://content.abcam.com/products/images/prmt6-antibody-pcrp-prmt6-2c9-ab277102--protein-array-img99554.jpg "Protein Array - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (ab277102)")
Protein Array - Anti-PRMT6 antibody [PCRP-PRMT6-2C9] (ab277102)
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277102.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com