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AB250433

Anti-PRMT7 antibody [EPR13495] - BSA and Azide free

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Rabbit Recombinant Monoclonal PRMT7 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

KIAA1933, PRMT7, Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7, [Myelin basic protein]-arginine N-methyltransferase PRMT7

1 Images
Western blot - Anti-PRMT7 antibody [EPR13495] - BSA and Azide free (AB250433)
  • WB

Supplier Data

Western blot - Anti-PRMT7 antibody [EPR13495] - BSA and Azide free (AB250433)

This data was developed using ab181214, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PRMT7 antibody [EPR13495] (<a href='/en-us/products/primary-antibodies/prmt7-antibody-epr13495-ab181214'>ab181214</a>) at 1/2000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 78 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13495

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250433 is the carrier-free version of ab181214.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRMT7 short for Protein Arginine Methyltransferase 7 plays an important role in the methylation of arginine residues on target proteins. It acts as a type of enzyme known for catalyzing the transfer of methyl groups. PRMT7 appears in several tissues although its expression is noted in higher amounts in brain and testis tissues. This protein weighs approximately 78 kDa and undergoes different modifications influencing its activity and localization. Researchers sometimes refer to PRMT7 simply as PRMT-7.
Biological function summary

The activity of this protein regulates gene expression by modifying histones and non-histone proteins affecting chromatin structure. It does not typically act as part of a large complex but can associate with other proteins to achieve specific functions. PRMT7 influences processes like protein signaling RNA processing and cell cycle control illustrating its diverse roles in cellular biology.

Pathways

PRMT7 participates intricately in pathways involving transcriptional regulation. It regulates pathways such as the arginine methylation pathway and is connected to transcription factor binding pathways. Its action intertwines with other methyltransferases and specific epigenetic regulators such as PRMT5 highlighting the crosstalk between different protein modification pathways within cells.

PRMT7 has a connection to cancer and neurodevelopmental disorders. Altered expression or activity of PRMT7 associates with oncogenesis making it a potential target in cancer research with some studies pointing out possible links to breast cancer. In neurodevelopmental disorders the protein's role in gene expression regulation suggests its involvement. In such conditions interactions with proteins such as FMR1 linked to Fragile X syndrome highlight the importance of understanding PRMT7's function to unravel its therapeutic implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
See full target information PRMT7
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Product promise

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