JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB168134

Anti-PRMT8 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Polyclonal PRMT8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRMT8.

View Alternative Names

HRMT1L3, HRMT1L4, PRMT8, Protein arginine N-methyltransferase 8, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4

1 Images
Western blot - Anti-PRMT8 antibody (AB168134)
  • WB

Unknown

Western blot - Anti-PRMT8 antibody (AB168134)

All lanes:

Western blot - Anti-PRMT8 antibody (ab168134) at 1 µg/mL

Lane 1:

PRMT8 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRMT8.

Q9NR22

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein arginine methyltransferase 8 commonly referred to as PRMT8 catalyzes the transfer of methyl groups to arginine residues on target proteins. It is part of the PRMT family and possesses a molecular mass of approximately 42 kDa. PRMT8 shares sequence similarity with PRMT1 but shows unique tissue-specific expression. Notably it is mainly found in the brain where it localizes to the plasma membrane suggesting specialized functions in neuronal cells.
Biological function summary

PRMT8 contributes to modulating signal transduction pathways in neurons. It plays a role in the post-translational modification of proteins which affects their interaction and stability. Though not known to form any stable large complexes PRMT8's methyltransferase activity modifies substrate proteins influencing their cellular functions. In neurons this activity may regulate synaptic plasticity or neural communication through methylation of proteins involved in signal transduction.

Pathways

Arginine methylation by PRMT8 influences several key signaling pathways including the phosphoinositide and protein kinase pathways. PRMT8's activity affects proteins such as Src family kinases which are important in transmitting signals for cell growth and differentiation. Interaction with these pathways suggests that PRMT8 serves as a modulator of complex cellular signaling mechanisms balancing neuronal response and adaptation.

Abnormalities in PRMT8 activity have associations with neurodegenerative diseases and brain-related disorders including Alzheimer's disease. Dysregulation of PRMT8 may affect amyloid precursor protein processing implicating its role along with proteins such as tau in disease pathology. Understanding PRMT8's involvement in these conditions could inform therapeutic strategies that target arginine methylation to ameliorate or prevent disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer (PubMed : 16051612, PubMed : 17925405, PubMed : 26529540, PubMed : 26876602). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS (PubMed : 18320585).
See full target information PRMT8
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of autoimmunity 97:10-21 PubMed30318155

2018

Arginine methylation of FOXP3 is crucial for the suppressive function of regulatory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Kagoya,Hiroshi Saijo,Yukiko Matsunaga,Tingxi Guo,Kayoko Saso,Mark Anczurowski,Chung-Hsi Wang,Kenji Sugata,Kenji Murata,Marcus O Butler,Cheryl H Arrowsmith,Naoto Hirano
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB271091

Anti-PRMT6 antibody [EPR24230-93]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRMT6 antibody [EPR24230-93] (AB271091)

  • 0
  • 0
Primary Antibodies

AB190892

Anti-PRMT1 antibody [EPR18344]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (AB190892)

  • 0
  • 0
Primary Antibodies

AB154154

Anti-PRMT2/HMT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PRMT2/HMT1 antibody (AB154154)

  • 0
  • 0
Primary Antibodies

AB109451

Anti-PRMT5 antibody [EPR5772]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody [EPR5772] (AB109451)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com