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Rabbit Recombinant Monoclonal Caspase-1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 310 publications.


Images

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515), expandable thumbnail
  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515), expandable thumbnail
  • Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515), expandable thumbnail
  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515), expandable thumbnail
  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255). Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003). In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).Isoform DeltaApoptosis inactive.Isoform EpsilonApoptosis inactive.

Alternative names

Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme, IL1BCE, IL1BC, CASP1

Recommended products

Rabbit Recombinant Monoclonal Caspase-1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 310 publications.

Alternative names

Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme, IL1BCE, IL1BC, CASP1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16883

Purification technique

Affinity purification Protein A

Specificity

This antibody has low affinity in Ms/Rt brain, H1299 and A549. 55kDa and 80kDa nonspecific bands may be observed in some samples.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

'Pro Caspase-1' is an inactive precursor of Caspase-1 also known as ICE (interleukin-1β-converting enzyme). It plays an important role in the process of inflammation and apoptosis. The pro-enzyme undergoes proteolytic processing to produce active Caspase-1 which consists of two subunits p10 and p20. The molecular weight of pro Caspase-1 is approximately 45 kDa. It is expressed in various tissues especially in immune cells like macrophages and monocytes. Expression levels can vary according to the inflammatory status of the tissue.

Biological function summary

Pro Caspase-1 is involved in the innate immune response by regulating the maturation of pro-inflammatory cytokines such as IL-1β and IL-18. This protein is a part of an essential multimeric complex known as the inflammasome. The inflammasome facilitates the activation of pro Caspase-1 leading to its conversion to active Caspase-1 initiating the cleavage and release of cytokines that signal the presence of infections and stress in cells.

Pathways

Pro Caspase-1 is integrated into the inflammation and pyroptosis pathways. It interacts with various proteins like ASC (apoptosis-associated speck-like protein containing a CARD) and NLRP3 to form the inflammasome complex which is important for cytokine processing and cell death mechanisms. These pathways demonstrate its significant role in limiting pathogen invasion and resolving inflammation through regulated cellular responses.

Associated diseases and disorders

Pro Caspase-1 is connected to several inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease. The activity of Caspase-1 regulated by its pro-form contributes to the pathogenesis of these disorders due to excessive inflammation. Additionally abnormal Caspase-1 activation implicates conditions like familial Mediterranean fever which often involve proteins like Pyrin in the inflammatory cascade. Understanding pro Caspase-1 dynamics helps in developing therapeutic strategies to modulate inflammation in these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515), expandable thumbnail

    Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: 7 seconds

    ab179515 has low affinity in Ms/Rt brain,H1299 and A549

    All lanes: Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Rat brain lysate at 20 µg

    Lane 3: NCI-H1299 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 6: Mouse spleen lysate at 20 µg

    Lane 7: Rat spleen lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515), expandable thumbnail

    Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20 and p10(p12) subunits. PMID: 8662843. The product recognizes p10(p12) cleavage fragment where the immunogen is located.

    All lanes: Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

    Lane 1: Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

    Lane 2: THP-1 (Human monocytic leukemia cell line) treated with 80nM TPA overnight, then treated with 1 μg/ml LPS for 8 hours whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa

    Observed band size: 10 kDa, 12 kDa, 35 kDa, 42 kDa, 45 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515), expandable thumbnail

    Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.

    Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate, 10μg (Input).

    Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab179515 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    Predicted band size: 45 kDa

  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515), expandable thumbnail

    Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.

    All lanes: Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

    Lane 1: Rat lung lysate at 20 µg

    Lane 2: Human fetal lung lysate at 20 µg

    Lane 3: J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate at 20 µg

    Lane 4: Rat spleen lysate at 20 µg

    Lane 5: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa

    Observed band size: 42 kDa, 45 kDa

  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515), expandable thumbnail

    Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    Different batches of ab179515 were tested on RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) lysate at 1.7 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 42,45 kDa.

    All lanes: Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    Predicted band size: 45 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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