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AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

3

(3 Reviews)

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(495 Publications)

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) is a rabbit monoclonal antibody detecting pro Caspase-1 + p10 + p12 in Western Blot, IP. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 310 publications

View Alternative Names

IL1BC, IL1BCE, CASP1, Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme

5 Images
Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)
  • WB

Supplier Data

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

Blocking and diluting buffer : 5% NFDM/TBST

Exposure time : 7 seconds

ab179515 has low affinity in Ms/Rt brain,H1299 and A549

All lanes:

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Lane 3:

NCI-H1299 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 6:

Mouse spleen lysate at 20 µg

Lane 7:

Rat spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 53 kDa

false

Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)
  • IP

Supplier Data

Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.

Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : RAW 264.7 whole cell lysate, 10μg (Input).

Lane 2 : ab179515 IP in RAW 264.7 whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab179515 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

Predicted band size: 45 kDa

false

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)
  • WB

Supplier Data

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds; Lane 2,3 and 4 : 10 seconds; Lane 5 : 8 seconds.

All lanes:

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

Lane 1:

Rat lung lysate at 20 µg

Lane 2:

Human fetal lung lysate at 20 µg

Lane 3:

J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate at 20 µg

Lane 4:

Rat spleen lysate at 20 µg

Lane 5:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 42 kDa,45 kDa

false

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)
  • WB

Supplier Data

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

Blocking/Dilution buffer : 5% NFDM/TBST.

There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20 and p10(p12) subunits. PMID : 8662843. The product recognizes p10(p12) cleavage fragment where the immunogen is located.

All lanes:

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

Lane 1:

Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 2:

THP-1 (Human monocytic leukemia cell line) treated with 80nM TPA overnight, then treated with 1 μg/ml LPS for 8 hours whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 10 kDa,12 kDa,35 kDa,42 kDa,45 kDa

false

Exposure time: 3min

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)
  • WB

Lab

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

Different batches of ab179515 were tested on RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) lysate at 1.7 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 42,45 kDa.

All lanes:

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

Predicted band size: 45 kDa

false

  • Carrier free

    Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16883

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has low affinity in Ms/Rt brain, H1299 and A549. 55kDa and 80kDa nonspecific bands may be observed in some samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IP and WB.

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) was first used in a scientific publication in 2017 and has been cited over 310 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) specifically detects pro Caspase-1 + p10 + p12 (UniProt ID: P29466; Molecular weight: 20kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR16883 - ab238972.



Pro-caspase-1, along with its subunits p10 and p12, plays a critical role in the activation of the inflammasome, which is involved in inflammatory responses and is linked to various diseases, including autoimmune disorders and infections

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Pro Caspase-1' is an inactive precursor of Caspase-1 also known as ICE (interleukin-1β-converting enzyme). It plays an important role in the process of inflammation and apoptosis. The pro-enzyme undergoes proteolytic processing to produce active Caspase-1 which consists of two subunits p10 and p20. The molecular weight of pro Caspase-1 is approximately 45 kDa. It is expressed in various tissues especially in immune cells like macrophages and monocytes. Expression levels can vary according to the inflammatory status of the tissue.
Biological function summary

Pro Caspase-1 is involved in the innate immune response by regulating the maturation of pro-inflammatory cytokines such as IL-1β and IL-18. This protein is a part of an essential multimeric complex known as the inflammasome. The inflammasome facilitates the activation of pro Caspase-1 leading to its conversion to active Caspase-1 initiating the cleavage and release of cytokines that signal the presence of infections and stress in cells.

Pathways

Pro Caspase-1 is integrated into the inflammation and pyroptosis pathways. It interacts with various proteins like ASC (apoptosis-associated speck-like protein containing a CARD) and NLRP3 to form the inflammasome complex which is important for cytokine processing and cell death mechanisms. These pathways demonstrate its significant role in limiting pathogen invasion and resolving inflammation through regulated cellular responses.

Pro Caspase-1 is connected to several inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease. The activity of Caspase-1 regulated by its pro-form contributes to the pathogenesis of these disorders due to excessive inflammation. Additionally abnormal Caspase-1 activation implicates conditions like familial Mediterranean fever which often involve proteins like Pyrin in the inflammatory cascade. Understanding pro Caspase-1 dynamics helps in developing therapeutic strategies to modulate inflammation in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed : 15326478, PubMed : 15498465, PubMed : 1574116, PubMed : 26375003, PubMed : 32051255, PubMed : 37993714, PubMed : 7876192, PubMed : 9334240). Plays a key role in cell immunity as an inflammatory response initiator : once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed : 15326478, PubMed : 15498465, PubMed : 1574116, PubMed : 32051255, PubMed : 7876192). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed : 15498465, PubMed : 1574116, PubMed : 7876192). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed : 26375003). In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed : 32051255, PubMed : 32109412, PubMed : 32553275). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed : 22464733). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed : 28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed : 20197547).. Isoform Delta. Apoptosis inactive.. Isoform Epsilon. Apoptosis inactive.
See full target information CASP1
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Publications (495)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:449 PubMed41057297

2025

Aging aggravated liver ischemia and reperfusion injury by promoting oxidized mtDNA mediated-macrophage pyroptosis through acetylated MCU-dependent calcium uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yi Wu,Rui Wang,Qi Zhang,Tao Liu,Jun-Yan Liu,Xue-Song Xu,Jun-Hua Gong

Journal of pharmaceutical analysis 15:101265 PubMed40979545

2025

Prioritization of potential drug targets for diabetic kidney disease using integrative omics data mining and causal inference.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu Zhang,Jie Peng,Chaolun Yu,Yu Ning,Wenhui Lin,Mingxing Ni,Qiang Xie,Chuan Yang,Huiying Liang,Miao Lin

Clinical and experimental pharmacology & physiology 52:e70074 PubMed40974203

2025

Activation of GPR39 Ameliorates Placental Dysfunction by Inhibiting Activation of NLRP1 Inflammasome in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohua Zhou,Hong Sun

Medical oncology (Northwood, London, England) 42:456 PubMed40883565

2025

Wedelolactone induces pyroptosis to suppress retinoblastoma by inhibiting the Nrf2/Keap1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Jiang,Hua Jiang,Guitao Wu,Ningdong Pang,Chuanqiang Niu,Zhouping Wang,Haibo Li

Nature communications 16:8127 PubMed40885754

2025

Pyroptosis-responsive microspheres modulate the inflammatory microenvironment to retard osteoporosis in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shunyi Lu,Jie Cao,Zhuorun Song,Fei Gong,Peng Yang,Jun Ge,Yunfei He,Zhihui Han,Guanghui Hou,Zimin Zhang,Yuqi Yang,Yun Teng,Zengli Zhang,Jun Zou,Liang Cheng,Huilin Yang

Foods (Basel, Switzerland) 14: PubMed40870677

2025

Yellow Pigments Ameliorate Hyperuricemia via Dual Mechanisms: Xanthine Oxidase Inhibition and Uric Acid Transporter Modulation (ABCG2, URAT1, and GLUT9).

Applications

Unspecified application

Species

Unspecified reactive species

Furong Xue,Renqin Zhu,Jiaxing Li,Zheng Liu,Lidan Niu,Wei Chen,Chengtao Wang,Jie Zheng

Theranostics 15:8658-8674 PubMed40860153

2025

Fe-flavonoid nanozyme as dual modulator of oxidative stress and autophagy for acute kidney injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Luo,Zhongsheng Xu,Chenxi Zhang,Zening Zhang,Pengchen Ren,Xiaojing He,Jingjing Zhang,Yun Liu

ACS bio & med chem Au 5:565-581 PubMed40860029

2025

A Potent Inhibitor of Caspase‑8 Based on the IL-18 Tetrapeptide Sequence Reveals Shared Specificities between Inflammatory and Apoptotic Initiator Caspases.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher M Bourne,Nicole R Raniszewski,Ashutosh B Mahale,Madhura Kulkarni,Patrick M Exconde,Sherry Liu,Winslow Yost,Tristan J Wrong,Robert C Patio,Matilda Kardhashi,Teni Shosanya,Mirai Kambayashi,Bohdana M Discher,Igor E Brodsky,George M Burslem,Cornelius Y Taabazuing

Acta biochimica et biophysica Sinica : PubMed40817715

2025

Succinate accumulation induces pyroptosis and mitochondrial damage via the inhibition of ATP5F1D in HUVECs.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Huang,Jian Cui,Dan Tang,Xing Xiang,Jie Mao,Zhe He,Hengjing Hu,Zhangxiu He,Lu He,Huifang Tang

American journal of reproductive immunology (New York, N.Y. : 1989) 94:e70132 PubMed40815040

2025

ATP/P2X4 Regulates Inflammation and Oxidative Stress in Endometriosis Through NLRP3 Inflammasome-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Yan Guo
View all publications
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