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Rabbit Recombinant Monoclonal Caspase-1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

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Images

Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (AB238972), expandable thumbnail
  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (AB238972), expandable thumbnail
  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (AB238972), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat, Human, Mouse

Dilution info

-

Notes

-

Target data

Function

Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:15326478, PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:26375003, PubMed:32051255). Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:1574116, PubMed:7876192, PubMed:15498465, PubMed:15326478, PubMed:32051255). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:1574116, PubMed:7876192, PubMed:15498465). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:26375003). In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32051255, PubMed:32109412, PubMed:32553275). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547).Isoform DeltaApoptosis inactive.Isoform EpsilonApoptosis inactive.

Alternative names

Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme, IL1BCE, IL1BC, CASP1

Recommended products

Rabbit Recombinant Monoclonal Caspase-1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR16883

Purification technique

Affinity purification Protein A

Specificity

This antibody has low affinity in Ms/Rt brain, H1299 and A549. 55kDa and 80kDa nonspecific bands may be observed in some samples.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab238972 is the carrier-free version of Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] ab179515.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

'Pro Caspase-1' is an inactive precursor of Caspase-1 also known as ICE (interleukin-1β-converting enzyme). It plays an important role in the process of inflammation and apoptosis. The pro-enzyme undergoes proteolytic processing to produce active Caspase-1 which consists of two subunits p10 and p20. The molecular weight of pro Caspase-1 is approximately 45 kDa. It is expressed in various tissues especially in immune cells like macrophages and monocytes. Expression levels can vary according to the inflammatory status of the tissue.

Biological function summary

Pro Caspase-1 is involved in the innate immune response by regulating the maturation of pro-inflammatory cytokines such as IL-1β and IL-18. This protein is a part of an essential multimeric complex known as the inflammasome. The inflammasome facilitates the activation of pro Caspase-1 leading to its conversion to active Caspase-1 initiating the cleavage and release of cytokines that signal the presence of infections and stress in cells.

Pathways

Pro Caspase-1 is integrated into the inflammation and pyroptosis pathways. It interacts with various proteins like ASC (apoptosis-associated speck-like protein containing a CARD) and NLRP3 to form the inflammasome complex which is important for cytokine processing and cell death mechanisms. These pathways demonstrate its significant role in limiting pathogen invasion and resolving inflammation through regulated cellular responses.

Associated diseases and disorders

Pro Caspase-1 is connected to several inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease. The activity of Caspase-1 regulated by its pro-form contributes to the pathogenesis of these disorders due to excessive inflammation. Additionally abnormal Caspase-1 activation implicates conditions like familial Mediterranean fever which often involve proteins like Pyrin in the inflammatory cascade. Understanding pro Caspase-1 dynamics helps in developing therapeutic strategies to modulate inflammation in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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