Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (AB238972)

pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.

Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : RAW 264.7 whole cell lysate, 10μg (Input).

Lane 2 : ab179515 IP in RAW 264.7 whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab179515 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179515).

All lanes:

Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (<a href='/en-us/products/primary-antibodies/pro-caspase-1-p10-p12-antibody-epr16883-ab179515'>ab179515</a>)

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (AB238972)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds; Lane 2,3 and 4 : 10 seconds; Lane 5 : 8 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab179515).

All lanes:

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (<a href='/en-us/products/primary-antibodies/pro-caspase-1-p10-p12-antibody-epr16883-ab179515'>ab179515</a>) at 1/1000 dilution

Lane 1:

Rat lung lysate at 20 µg

Lane 2:

Human fetal lung lysate at 20 µg

Lane 3:

J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate at 20 µg

Lane 4:

Rat spleen lysate at 20 µg

Lane 5:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 42 kDa,45 kDa

false

• WB

Lab

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] - BSA and Azide free (AB238972)

This data was developed using ab179515, the same antibody clone in a different buffer formulation. Different batches of ab179515 were tested on RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) lysate at 1.7 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 42,45 kDa.

All lanes:

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (<a href='/en-us/products/primary-antibodies/pro-caspase-1-p10-p12-antibody-epr16883-ab179515'>ab179515</a>)

Predicted band size: 45 kDa

false