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AB32499

Anti-pro Caspase-3 antibody [E83-103]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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(122 Publications)

Rabbit Recombinant Monoclonal Caspase-3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 122 publications.

View Alternative Names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

3 Images
Flow Cytometry (Intracellular) - Anti-pro Caspase-3 antibody [E83-103] (AB32499)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-pro Caspase-3 antibody [E83-103] (AB32499)

Overlay histogram showing Jurkat cells stained with ab32499 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32499, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was anti-rabbit DyLight® 488 (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions.

Acquisition of >5,000 events was performed.

Western blot - Anti-pro Caspase-3 antibody [E83-103] (AB32499)
  • WB

Lab

Western blot - Anti-pro Caspase-3 antibody [E83-103] (AB32499)

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 3 : Caspase-3 knockout HAP1 cell lysate
Lane 4 : Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32499 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32499 was shown to specifically react with pro Caspase 3 when Caspase 3 knockout samples were used. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab32499 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-pro Caspase-3 antibody [E83-103] (ab32499)

Predicted band size: 31 kDa

Observed band size: 35 kDa

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Western blot - Anti-pro Caspase-3 antibody [E83-103] (AB32499)
  • WB

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Western blot - Anti-pro Caspase-3 antibody [E83-103] (AB32499)

All lanes:

Western blot - Anti-pro Caspase-3 antibody [E83-103] (ab32499) at 1/10000 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

Jurkat cell lysate + Camptothecin

Predicted band size: 31 kDa

Observed band size: 35 kDa

false

  • Carrier free

    Anti-pro Caspase-3 antibody [E83-103] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E83-103

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro Caspase-3 also known as Caspase-3 zymogen is a central component in the apoptotic process. This inactive precursor form undergoes cleavage during apoptosis to become active caspase-3 an important executioner in cell death. Caspase-3 possesses a molecular weight of approximately 32 kDa. It is expressed ubiquitously in cells throughout the body with a higher concentration in tissues with rapid cell turnover such as thymus and gastrointestinal tract.
Biological function summary

The inactive pro Caspase-3 form serves as a safeguard in cells preventing premature apoptosis. It only becomes the active caspase-3 after specific activation ensuring controlled progression of apoptosis. This inactive form does not participate in any complex but becomes a part of the caspase family network upon cleavage. In its active form caspase-3 leads to the cleavage of several key cellular proteins driving the morphological and biochemical changes seen during apoptosis.

Pathways

Pro Caspase-3 is critical in the apoptotic signaling pathway. It serves as a link between intrinsic and extrinsic pathways of apoptosis. The intrinsic pathway often triggered by mitochondrial signals and the extrinsic pathway initiated by death receptor signals both lead to the activation of caspase-3. In these pathways pro Caspase-3 interacts with proteins like caspase-8 and cytochrome c playing a part in their signaling cascades and cellular outcomes.

Pro Caspase-3 is associated with conditions involving dysregulated apoptosis. One such condition is cancer where evasion of apoptosis leads to uncontrolled cell proliferation. The therapeutic targeting of pro Caspase-3 activation holds potential in reinstating apoptotic pathways in cancerous cells. Additionally it relates to neurodegenerative diseases like Alzheimer's where excessive apoptosis contributes to neuronal loss. Here active caspase-3 interacts with proteins like amyloid precursor protein suggesting a role in the disease's progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 35338844, PubMed : 35446120, PubMed : 7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed : 10497198, PubMed : 16374543, PubMed : 7596430, PubMed : 7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed : 7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed : 37993714, PubMed : 9334240). Involved in the cleavage of huntingtin (PubMed : 8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed : 21357690). Cleaves DSG2 in response to apoptosis resulting in a loss of full length DSG2 at desmosome cell junctions and subsequent loss of cell-cell adhesion (PubMed : 17559062). Also cleaves JUP in response to apoptosis (PubMed : 17559062). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed : 23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed : 35338844, PubMed : 35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed : 23845944, PubMed : 33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758104, PubMed : 36758106).
See full target information CASP3
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Publications (122)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 18:87 PubMed40287776

2025

Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Gao,Yeke Wu,Yuqin Tang,Keming Wu,Baojun Guo

Frontiers in pharmacology 16:1519497 PubMed40012630

2025

Sinensetin attenuates hepatic ischemia-reperfusion injury through suppressing GRP78/CHOP-mediated endoplasmic reticulum (ER) stress in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yihong Yang,Guanghua Xiong,Huangqi Shi,Yulin Peng,Jinghong Liu,Yaxuan Jiang,Min Lu,Hongbo Liu,Yong Liu

Scientific reports 14:24962 PubMed39438537

2024

Synergistic effects of bloom helicase (BLM) inhibitor AO/854 with cisplatin in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Ma,Fu Tian,Yuanpin Xiao,Mengqiu Huang,Dandan Song,Xinlin Chen,Houqiang Xu

mSystems 9:e0089324 PubMed39240104

2024

Activation of NF-κB/MAPK signaling and induction of apoptosis by salicylate synthase NbtS in promotes neuroinflammation development.

Applications

Unspecified application

Species

Unspecified reactive species

Jirao Shen,Xing Zhao Ji,Lichao Han,Jiang Yao,Yihe Liang,Min Yuan,Shuai Xu,Zhenjun Li

Redox report : communications in free radical research 29:2382943 PubMed39092597

2024

Hyperglycemia-induced oxidative stress exacerbates mitochondrial apoptosis damage to cochlear stria vascularis pericytes via the ROS-mediated Bcl-2/CytC/AIF pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Feng Shi,Zan Zhou,Wen-Jun Jiang,Tian-Lan Huang,Jun-Qiang Si,Li Li

Neural regeneration research 20:858-872 PubMed38886958

2024

Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Xintian Ding,Chun Chen,Heng Zhao,Bin Dai,Lei Ye,Tao Song,Shuai Huang,Jia Wang,Tao You

Journal of orthopaedic surgery and research 19:309 PubMed38783358

2024

Local Application of Tanshinone IIA protects mesenchymal stem cells from apoptosis and promotes fracture healing in ovariectomized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shao Cheng,Xiaohui Hu,Kanghui Sun,Ziyu Huang,Yongjian Zhao,Yueli Sun,Bo Zeng,Jing Wang,Dongfeng Zhao,Sheng Lu,Qi Shi,Yongjun Wang,Weian Zhang,Xinhua Liu,Bing Shu

Experimental hematology & oncology 13:30 PubMed38468359

2024

Suppression of A-to-I RNA-editing enzyme ADAR1 sensitizes hepatocellular carcinoma cells to oxidative stress through regulating Keap1/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Houhong Wang,Xiaoyu Wei,Lu Liu,Junfeng Zhang,Heng Li

Acta biochimica et biophysica Sinica 56:607-620 PubMed38414350

2024

miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqing Jiang,Junmei Xu,Hua Zeng,Zhaojing Lin,Qiong Yi,Jiali Guo,Feng Xiao

Journal of neuroimmunology 388:578309 PubMed38335781

2024

Caveolin-1 mediates blood-brain barrier permeability, neuroinflammation, and cognitive impairment in SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Troy N Trevino,Ali A Almousawi,KaReisha F Robinson,Avital B Fogel,Jake Class,Richard D Minshall,Leon M Tai,Justin M Richner,Sarah E Lutz
View all publications
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Product promise

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