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AB238440

Anti-pro Caspase-3 antibody [E83-103] - BSA and Azide free

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(6 Publications)

Rabbit Recombinant Monoclonal Caspase-3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

2 Images
Flow Cytometry (Intracellular) - Anti-pro Caspase-3 antibody [E83-103] - BSA and Azide free (AB238440)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-pro Caspase-3 antibody [E83-103] - BSA and Azide free (AB238440)

Overlay histogram showing Jurkat cells stained with ab32499 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32499, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was anti-rabbit DyLight® 488 (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32499)

Western blot - Anti-pro Caspase-3 antibody [E83-103] - BSA and Azide free (AB238440)
  • WB

Lab

Western blot - Anti-pro Caspase-3 antibody [E83-103] - BSA and Azide free (AB238440)

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 3 : Caspase-3 knockout HAP1 cell lysate
Lane 4 : Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32499 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32499 was shown to specifically react with pro Caspase 3 when Caspase 3 knockout samples were used. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab32499 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab32499).

All lanes:

Western blot - Anti-pro Caspase-3 antibody [E83-103] (<a href='/en-us/products/primary-antibodies/pro-caspase-3-antibody-e83-103-ab32499'>ab32499</a>)

Predicted band size: 31 kDa

Observed band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E83-103

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects pro-form (35kD) of caspase 3, and does not recognize any cleaved caspases.

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Reactivity data

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Product details

ab238440 is the carrier-free version of ab32499.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro Caspase-3 also known as Caspase-3 zymogen is a central component in the apoptotic process. This inactive precursor form undergoes cleavage during apoptosis to become active caspase-3 an important executioner in cell death. Caspase-3 possesses a molecular weight of approximately 32 kDa. It is expressed ubiquitously in cells throughout the body with a higher concentration in tissues with rapid cell turnover such as thymus and gastrointestinal tract.
Biological function summary

The inactive pro Caspase-3 form serves as a safeguard in cells preventing premature apoptosis. It only becomes the active caspase-3 after specific activation ensuring controlled progression of apoptosis. This inactive form does not participate in any complex but becomes a part of the caspase family network upon cleavage. In its active form caspase-3 leads to the cleavage of several key cellular proteins driving the morphological and biochemical changes seen during apoptosis.

Pathways

Pro Caspase-3 is critical in the apoptotic signaling pathway. It serves as a link between intrinsic and extrinsic pathways of apoptosis. The intrinsic pathway often triggered by mitochondrial signals and the extrinsic pathway initiated by death receptor signals both lead to the activation of caspase-3. In these pathways pro Caspase-3 interacts with proteins like caspase-8 and cytochrome c playing a part in their signaling cascades and cellular outcomes.

Pro Caspase-3 is associated with conditions involving dysregulated apoptosis. One such condition is cancer where evasion of apoptosis leads to uncontrolled cell proliferation. The therapeutic targeting of pro Caspase-3 activation holds potential in reinstating apoptotic pathways in cancerous cells. Additionally it relates to neurodegenerative diseases like Alzheimer's where excessive apoptosis contributes to neuronal loss. Here active caspase-3 interacts with proteins like amyloid precursor protein suggesting a role in the disease's progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 35338844, PubMed : 35446120, PubMed : 7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed : 10497198, PubMed : 16374543, PubMed : 7596430, PubMed : 7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed : 7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed : 37993714, PubMed : 9334240). Involved in the cleavage of huntingtin (PubMed : 8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed : 21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed : 23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed : 35338844, PubMed : 35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed : 23845944, PubMed : 33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758104, PubMed : 36758106).
See full target information CASP3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:794-802 PubMed37782689

2023

HO-3867, a curcumin analog, elicits cell apoptosis and p38-mediated caspase activation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan-Chun Hsueh,Po-Chung Ju,Yi-Hsien Hsieh,Shih-Chi Su,Chao-Bin Yeh,Chiao-Wen Lin

Journal of cellular and molecular medicine 27:1341-1352 PubMed37029501

2023

NDUFV1 attenuates renal ischemia-reperfusion injury by improving mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Li,Lingling Zhang,Yingjie Cao,Xu Chen,Haifeng Gong,Yidan Ma,Yuanyuan Gui,Tianya Xiang,Jianxing Liu,Xinzhong Huang

Artificial cells, nanomedicine, and biotechnology 48:506-514 PubMed32024386

2020

Dexmedetomidine protects PC12 cells from oxidative damage through regulation of miR-199a/HIF-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Yalin Xi,Qinglong Kong

Technology in cancer research & treatment 18:1533033819892258 PubMed31870221

2019

DDX5 Silencing Suppresses the Migration of Basal cell Carcinoma Cells by Downregulating JAK2/STAT3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Quan,Bei-Bei Zhang,Fang Yin,Jiru Du,Yuan-Ting Zhi,Jin Xu,Ningjing Song

Journal of cellular biochemistry 121:259-268 PubMed31222827

2019

LncRNA-LET relieves hypoxia-induced injury in H9c2 cells through regulation of miR-138.

Applications

Unspecified application

Species

Unspecified reactive species

Yugeng Li,Jianwei Li,Pengzhen Zhang,Xiaoying Jiang,Zhenrui Pan,Wenjian Zheng,Hongli Lin

Journal of cellular biochemistry 120:14679-14687 PubMed31009136

2019

Sapylin inhibits lung cancer cell proliferation and promotes apoptosis by attenuating PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Benhong Liu
View all publications
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