Rabbit Recombinant Monoclonal Collagen III antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Rat, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104483.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082)")
![Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-frozen-sections-img109041.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82567.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082)")
![Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-frozen-sections-img122986.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (AB278082)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | WB | ICC/IF | IHC-P | Flow Cyt | IP | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Alternative names
Collagen alpha-1(III) chain, Col3a1
Recommended products
Rabbit Recombinant Monoclonal Collagen III antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24257-57
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognizes Pro-Collagen III alpha 1.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab278082 is the carrier-free version of Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pro-Collagen III alpha 1 also known as collagen III is a precursor to type III collagen. It has a molecular mass of approximately 139 kDa. This protein predominantly occurs in tissues like skin lung intestine and the vascular system where it plays a mechanical role by contributing to the structural integrity and mechanical properties of these organs. The synthesize beings in the fibroblasts and procollagen undergoes a series of post-translational modifications before it secreted into the extracellular matrix.
- Biological function summary
This precursor form of collagen functions as part of the collagen formation process. As a component of the extracellular matrix it interacts with various components like glycosaminoglycans and elastin helping maintain elasticity and strength. Pro-collagen III is not a part of oligomeric complexes but rather after processing joins the fibrillar collagens to support tissue structure and repair.
- Pathways
Pro-Collagen III alpha 1 plays a role in the processes of collagen biosynthesis and fibroblast growth signaling. It participates in the extracellular matrix organization pathway where it influences cellular adhesion migration and differentiation. This protein is related to other proteins such as collagen I and fibronectin through these pathways highlighting its importance in tissue integrity and wound healing.
- Associated diseases and disorders
The overproduction or dysfunction of Pro-Collagen III alpha 1 or its matured form collagen III often relates to fibrotic diseases and vascular conditions like Ehlers-Danlos syndrome. It interacts with collagen I in these scenarios which together can impact the phenotype of affected tissues leading to abnormalities in skin elasticity and cardiovascular complications. Understanding its role in these diseases helps in targeted therapies and potential treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104483.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)
This data was developed using Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Pro-Collagen III alpha 1 with Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080 at 1/2000 (0.253 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of mouse kidney. The section was incubated with Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-frozen-sections-img109041.jpg "Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)")
Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)
This data was developed using Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling Pro-Collagen III alpha 1 with Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080 at 1/250 (2.024 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82567.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)
This data was developed using Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Pro-Collagen III alpha 1 with Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080 at 1/2000 (0.253 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of rat kidney. The section was incubated with Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082), expandable thumbnail](https://content.abcam.com/products/images/pro-collagen-iii-alpha-1-antibody-epr24257-57-bsa-and-azide-free-ab278082--immunohistochemistry-frozen-sections-img122986.jpg "Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)")
Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] - BSA and Azide free (ab278082)
This data was developed using Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cardiac muscle tissue labeling Pro-Collagen III alpha 1 with Anti-Pro-Collagen III alpha 1 antibody [EPR24257-57] ab278080 at 1/250 (2.024 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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