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Rabbit Recombinant Multiclonal Collagen III antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (AB283694), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (AB283694), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (AB283694), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (AB283694), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (AB283694), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PIHC-Fr
Human
Not recommended
Tested
Tested
Tested
Expected
Mouse
Not recommended
Expected
Expected
Tested
Tested
Rat
Not recommended
Expected
Expected
Tested
Tested

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Alternative names

Collagen alpha-1(III) chain, Col3a1

Recommended products

Rabbit Recombinant Multiclonal Collagen III antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM1028
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pro-Collagen III alpha 1 also known as collagen III is a precursor to type III collagen. It has a molecular mass of approximately 139 kDa. This protein predominantly occurs in tissues like skin lung intestine and the vascular system where it plays a mechanical role by contributing to the structural integrity and mechanical properties of these organs. The synthesize beings in the fibroblasts and procollagen undergoes a series of post-translational modifications before it secreted into the extracellular matrix.

Biological function summary

This precursor form of collagen functions as part of the collagen formation process. As a component of the extracellular matrix it interacts with various components like glycosaminoglycans and elastin helping maintain elasticity and strength. Pro-collagen III is not a part of oligomeric complexes but rather after processing joins the fibrillar collagens to support tissue structure and repair.

Pathways

Pro-Collagen III alpha 1 plays a role in the processes of collagen biosynthesis and fibroblast growth signaling. It participates in the extracellular matrix organization pathway where it influences cellular adhesion migration and differentiation. This protein is related to other proteins such as collagen I and fibronectin through these pathways highlighting its importance in tissue integrity and wound healing.

Associated diseases and disorders

The overproduction or dysfunction of Pro-Collagen III alpha 1 or its matured form collagen III often relates to fibrotic diseases and vascular conditions like Ehlers-Danlos syndrome. It interacts with collagen I in these scenarios which together can impact the phenotype of affected tissues leading to abnormalities in skin elasticity and cardiovascular complications. Understanding its role in these diseases helps in targeted therapies and potential treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cardiac muscle (fresh) tissue labeling COL3A1 with ab283694 at 1/100 (6.29 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cardiac muscle (fresh) tissue labeling COL3A1 with ab283694 at 1/100 (6.29 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on mouse cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa cells (Human cervix adenocarcinoma epithelial cell) labelling COL3A1 with ab283694 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling COL3A1 with ab283694 at 1/100 (6.29 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the rat kidney. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the mouse kidney. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the human colon carcinoma. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] (ab283694)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labelling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the human endometrium. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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