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AB283725

Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free

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Rabbit Recombinant Multiclonal Collagen III antibody. Carrier free. Suitable for IHC-P, IHC-Fr, Flow Cyt (Intra), ICC/IF and reacts with Rat, Mouse, Human samples.

View Alternative Names

Collagen alpha-1(III) chain, Col3a1

8 Images
Flow Cytometry (Intracellular) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling COL3A1 with ab283694 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labelling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the human endometrium. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling COL3A1 with ab283694 at 1/100 (6.29 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the human colon carcinoma. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the mouse kidney. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling COL3A1 with ab283694 at 1/1000 (0.629 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the stroma in the rat kidney. The section was incubated with ab283694 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cardiac muscle (fresh) tissue labeling COL3A1 with ab283694 at 1/100 (6.29 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Pro-Collagen III alpha 1 antibody [RM1028] - BSA and Azide free (AB283725)

This data was developed using ab283694, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cardiac muscle (fresh) tissue labeling COL3A1 with ab283694 at 1/100 (6.29 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on mouse cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

  • Unconjugated

    Anti-Pro-Collagen III alpha 1 antibody [RM1028]

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1028

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

Flow Cyt (Intra), IHC-Fr, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB320800

Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit (AB320800)

  • 0
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Reagents, Controls & Accessories

AB246832

Picro-Sirius Red Solution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

  • 0
  • 0
Primary Antibodies

AB34710

Anti-Collagen I + Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

  • 5
  • 91
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB260043

Anti-Collagen I antibody [EPR22894-89]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043)

  • 4
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab283725 is the carrier-free version of ab283694.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro-Collagen III alpha 1 also known as collagen III is a precursor to type III collagen. It has a molecular mass of approximately 139 kDa. This protein predominantly occurs in tissues like skin lung intestine and the vascular system where it plays a mechanical role by contributing to the structural integrity and mechanical properties of these organs. The synthesize beings in the fibroblasts and procollagen undergoes a series of post-translational modifications before it secreted into the extracellular matrix.
Biological function summary

This precursor form of collagen functions as part of the collagen formation process. As a component of the extracellular matrix it interacts with various components like glycosaminoglycans and elastin helping maintain elasticity and strength. Pro-collagen III is not a part of oligomeric complexes but rather after processing joins the fibrillar collagens to support tissue structure and repair.

Pathways

Pro-Collagen III alpha 1 plays a role in the processes of collagen biosynthesis and fibroblast growth signaling. It participates in the extracellular matrix organization pathway where it influences cellular adhesion migration and differentiation. This protein is related to other proteins such as collagen I and fibronectin through these pathways highlighting its importance in tissue integrity and wound healing.

The overproduction or dysfunction of Pro-Collagen III alpha 1 or its matured form collagen III often relates to fibrotic diseases and vascular conditions like Ehlers-Danlos syndrome. It interacts with collagen I in these scenarios which together can impact the phenotype of affected tissues leading to abnormalities in skin elasticity and cardiovascular complications. Understanding its role in these diseases helps in targeted therapies and potential treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information Collagen alpha-1(III) chain
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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