AB206106

Anti-pro-EGF antibody [EPR14622]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal EGF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Pro-epidermal growth factor, EGF

5 Images

Supplier Data

Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (AB206106)

EGF was immunoprecipitated from 1mg of Human milk sample with ab206106 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206106 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human milk sample 10ug (Input).

Lane 2 : ab206106 IP in Human milk sample.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206106 in Human milk sample.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (ab206106)

false

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

All lanes:

Human urine sample at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 1 second

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

Lane 1:

Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag at 0.015 µg

Lane 2:

Recombinant Human EGF recombinant protein (aa971-1023) at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

false

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human EGF full length protein contains aa21-1023 with His-Tag® at the C-Terminus.

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/5000 dilution

All lanes:

Human EGF recombinant protein at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

All lanes:

Human milk sample at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14622

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognises the Pro-epidermal growth factor chain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro-epidermal growth factor (pro-EGF) is a precursor form of the epidermal growth factor often referred to as EGF precursor. This protein has a mass of approximately 175 kDa and is known for its role in cell signaling. Pro-EGF is expressed in various tissues including the kidney salivary glands and the gastrointestinal tract. Upon cleavage it releases the smaller active form of EGF which then modulates numerous cellular processes by binding to the EGF receptor (EGFR).

Biological function summary

Pro-EGF plays a critical role in cellular growth and differentiation. Through its conversion to active EGF it interacts with the EGF receptor initiating a cascade of signaling events. This process regulates processes such as inflammation wound healing and embryonic development. Pro-EGF does not form part of a higher-order complex but acts through interactions with its receptor to elicit biological responses.

Pathways

Pro-EGF's function is integral to the MAPK/ERK signaling pathway. It activates this pathway through EGFR binding promoting cellular proliferation and differentiation processes. Pro-EGF also participates in the PI3K/AKT signaling pathway influencing cell survival and growth. Its interaction with EGFR is closely related to other growth factors like TGF-alpha which share the same receptor binding activity.

Pro-EGF has been linked to cancer and wound healing disorders. Dysregulation of EGF signaling often due to overexpression or mutation of EGFR contributes to various cancers like non-small cell lung cancer. Pro-EGF's connection with EGFR is also critical in chronic wounds where impaired signaling can delay healing. Understanding these interactions helps inform therapeutic strategies targeting EGFR in cancer and regenerative medicine.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed : 10964941).

See full target information EGF

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:20600 PubMed40594542

2025

TRPV1 inhibition sensitizes tumors to PD-1 blockade by reversing resistance to CTL-mediated killing.

Applications

Unspecified application

Species

Unspecified reactive species

Min Kyu Son,Hyo-Jung Lee,Eunho Cho,Se Jin Oh,Tae Woo Kim

Communications biology 7:1700 PubMed39725699

2024

MSC-derived exosomal circMYO9B accelerates diabetic wound healing by promoting angiogenesis through the hnRNPU/CBL/KDM1A/VEGFA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Hongbo Xu,Bichen Xue,Lian Liu,Yulin Tang,Zhichao Wang,Kai Yao

Frontiers in oncology 14:1470379 PubMed39469640

2024

Modeling epithelial-mesenchymal transition in patient-derived breast cancer organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Neta Bar-Hai,Rakefet Ben-Yishay,Sheli Arbili-Yarhi,Naama Herman,Vered Avidan-Noy,Tehillah Menes,Aiham Mansour,Fahim Awwad,Nora Balint-Lahat,Gil Goldinger,Goni Hout-Siloni,Mohammad Adileh,Raanan Berger,Dana Ishay-Ronen

Nature communications 14:2691 PubMed37165076

2023

TRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Se Jin Oh,Ji Yeon Lim,Min Kyu Son,Jun Hyeok Ahn,Kwon-Ho Song,Hyo-Jung Lee,Suyeon Kim,Eun Ho Cho,Joon-Yong Chung,Hanbyoul Cho,Hyosun Kim,Jae-Hoon Kim,Jooyoung Park,Jungmin Choi,Sun Wook Hwang,Tae Woo Kim

Autophagy 17:1978-1997 PubMed32762616

2020

LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Suyeon Kim,Hanbyoul Cho,Soon-Oh Hong,Se Jin Oh,Hyo-Jung Lee,Eunho Cho,Seon Rang Woo,Joon Seon Song,Joon-Yong Chung,Sung Wook Son,Sang Min Yoon,Yu-Min Jeon,Seunghyun Jeon,Cassian Yee,Kyung-Mi Lee,Stephen M Hewitt,Jae-Hoon Kim,Kwon-Ho Song,Tae Woo Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-pro-EGF antibody [EPR14622]

Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (AB206106)

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Western blot - Anti-pro-EGF antibody [EPR14622] (AB206106)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Product promise