JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB252641

Anti-Pro-GRP (31-98) antibody [EPR20057-3] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal GRP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Gastrin-releasing peptide, GRP

1 Images
Sandwich ELISA - Anti-Pro-GRP (31-98) antibody [EPR20057-3] - BSA and Azide free (Detector) (AB252641)
  • sELISA

Lab

Sandwich ELISA - Anti-Pro-GRP (31-98) antibody [EPR20057-3] - BSA and Azide free (Detector) (AB252641)

Sandwich ELISA of ab253576 with the capture antibody ab252582 dilution at 2 µg/mL and detector antibody ab252641 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20057-3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab252641 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Pro-Gastrin-releasing Peptide (ProGRP31-98). The recommended pair for sandwich ELISA is:
Capture: ab252582, Human Pro-Gastrin-releasing Peptide (ProGRP31-98) Capture Antibody (unconjugated)
Detector: ab252641, Human Pro-Gastrin-releasing Peptide (ProGRP31-98) Detector Antibody (unconjugated)
The reference range value is 15.63-1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro-GRP (31-98) also known as Progastrin-releasing peptide is a peptide with a mass of approximately 8 kDa. It serves as a precursor for gastrin-releasing peptide (GRP). It undergoes cleavage to form the active GRP peptide. GRP belongs to the bombesin-like peptide family and you can primarily find it expressed in neuroendocrine tissues particularly in the gastrointestinal tract and lungs. This peptide plays an important role in various cellular signaling processes by binding to its receptors.
Biological function summary

The gastrin-releasing peptide influences the release of important hormones such as gastrin. It acts on the gastrointestinal system contributing to the stimulation of gastric acid secretion. Additionally GRP has a role in neurotransmission. It forms part of a signaling system that operates in neuroendocrine communication where it works with other neuropeptides to regulate physiological processes.

Pathways

The gastrin-releasing peptide impacts several signaling pathways including the gastrin secretion pathway and the bombesin receptor pathway. It interacts with receptors on target cells to induce cellular responses including growth and secretory functions. Related proteins like the bombesin receptor subtype 3 are involved in these pathways modulating different biochemical reactions that contribute to digestion and central nervous system signaling.

Gastrin-releasing peptide has associations with small cell lung cancer and gastrointestinal disorders. Elevated levels of Pro-GRP (31-98) can serve as a biomarker for small cell lung cancer aiding in diagnosis and monitoring treatment responses. The interplay of GRP with the bombesin receptor and related pathways like those involving gastrin underlines its importance in certain tumorigenic processes and dysregulation in gastrointestinal functions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Stimulates the release of gastrin and other gastrointestinal hormones (By similarity). Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior (By similarity). Contributes primarily to nonhistaminergic itch sensation (By similarity). In one study, shown to act in the amygdala as part of an inhibitory network which inhibits memory specifically related to learned fear (By similarity). In another study, shown to act on vasoactive intestinal peptide (VIP)-expressing cells in the auditory cortex, most likely via extrasynaptic diffusion from local and long-range sources, to mediate disinhibition of glutamatergic cells via VIP cell-specific GRPR signaling which leads to enhanced auditory fear memories (By similarity). Contributes to the regulation of food intake (By similarity). Inhibits voltage-gated sodium channels but enhances voltage-gated potassium channels in hippocampal neurons (By similarity). Induces sighing by acting directly on the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity (By similarity).. Neuromedin-C. Induces an itch response through activation of receptors present on mast cells, triggering mast cell degranulation.
See full target information Gastrin-releasing peptide
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB315304

Human Pro-GRP (31-98) ELISA Kit- extracellular

SimpleStep

Sandwich ELISA - Human Pro-GRP (31-98) ELISA Kit- extracellular (AB315304)

  • 0
  • 0
Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 37
Primary Antibodies

AB181547

Anti-Insulin antibody [EPR17359]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Insulin antibody [EPR17359] (AB181547)

  • 5
  • 11
Primary Antibodies

AB92517

Anti-Glucagon antibody [EP3070]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)

  • 5
  • 8
Primary Antibodies

AB223501

Anti-Gastrin antibody [SP318]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (AB223501)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com