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AB281141

Anti-Pro-MCH antibody [EPR23778-68] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal MCH antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

MCH, PMCH, Pro-MCH

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Sandwich ELISA - Anti-Pro-MCH antibody [EPR23778-68] - BSA and Azide free (Capture) (AB281141)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Pro-MCH antibody [EPR23778-68] - BSA and Azide free (Capture) (AB281141)

Example of Human Pro-MCH standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23778-68

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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You may be interested in:

AB303757

Human Pro-MCH ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab281141 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PMCH. The recommended pair for sandwich ELISA is:
Capture: ab281141, Human PMCH Capture Antibody (unconjugated)
Detector: ab280991, Human PMCH Detector Antibody (unconjugated)
The reference range value is 39 - 10000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pro-MCH also known as pro-melanin-concentrating hormone is a precursor polypeptide that undergoes post-translational processing to produce mature melanin-concentrating hormone (MCH). The precursor weighs approximately 18 kDa before processing. Pro-MCH is expressed mainly in the hypothalamus but can also be found in other brain regions involved in regulating appetite and energy homeostasis.
Biological function summary

The pro-MCH precursor undergoes cleavage to generate MCH which plays a significant role in energy balance and feeding behavior. MCH functions are often linked to neuronal populations in the hypothalamus that regulate hunger signals and it is involved in forming neuronal circuits that influence behavior related to food intake. Although pro-MCH itself is not part of a larger complex the derived MCH interacts with its receptors to transmit signals.

Pathways

Melanin-concentrating hormone interacts with energy homeostasis and reward circuits in the brain. It is engaged in the melanocortin pathway which balances energy intake and expenditure. MCH modulates signaling by interacting with receptors such as MCHR1 and MCHR2 influencing food-seeking behavior and energy regulation processes. Additionally its regulation aligns with orexigenic (appetite-increasing) peptides such as neuropeptide Y (NPY) coordinating energy-related responses.

Aberrant MCH signaling relates to metabolic disorders such as obesity and altered response to stress. Individuals with irregular MCH signaling may exhibit increased appetite and weight gain contributing to metabolic syndrome. The interaction with MCHR1 is significant here as alterations in this receptor are associated with such metabolic changes. Furthermore pro-MCH and its derivative peptides have also been linked to mood disorders with disrupted MCH pathways influencing depression-related behaviors through interactions with proteins like corticotropin-releasing hormone (CRH).
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
See full target information PMCH
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com