AB13111

Anti-proBNP antibody [5B6]

Name: Anti-proBNP antibody [5B6] (ab13111) | Abcam
Brand: Abcam Limited
SKU: AB13111
Price: 525 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal BNP antibody. Suitable for ELISA, WB and reacts with Synthetic peptide samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 1-50.

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

2 Images

ELISA - Anti-proBNP antibody [5B6] (AB13111)

ELISA

Unknown

ELISA - Anti-proBNP antibody [5B6] (AB13111)

NT-proBNP calibration curves using pairs ab14703 [10H4] and ab13111 [5B6] (grey), and ab13115 [15F11] and ab13111 [5B6] (black), (capture : 2 μg/well, detection : Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature

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Western blot - Anti-proBNP antibody [5B6] (AB13111)

WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].

Lane 1:

Western blot - Anti-NT-proBNP antibody [15F11] (<a href='/en-us/products/primary-antibodies/nt-probnp-antibody-15f11-ab13115'>ab13115</a>)

Lane 1:

Western blot - Anti-proBNP antibody [5B6] (ab13111)

Predicted band size: 15 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B6

Isotype

IgG1

Carrier free

Applications

ELISA, WB

applications

Immunogen

Synthetic Peptide within Human NPPB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16860

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This product has switched from ascites to TCS on 10th June 2020.

Immunoglobulin concentrations vary from batch to batch (generally 4-6 mg/ml for this clone). Please contact technical services for the latest information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proBNP also known by names like NT-proBNP and brain natriuretic peptide precursor is a peptide that plays an important role as a cardiac biomarker. This protein has a molecular mass of about 13 kDa and is expressed primarily in cardiac tissues especially the ventricles. ProBNP is synthesized as a precursor and then processed to produce active BNP (B-type natriuretic peptide) and the inactive part NT-proBNP which can be used for diagnosis purposes.

Biological function summary

ProBNP contributes significantly to cardiovascular homeostasis by promoting natriuresis and diuresis which help in reducing blood pressure and volume. It does not form part of a larger protein complex but functions both in the formation of its active peptide BNP and its inactive fragment NT-proBNP. By exerting these effects proBNP influences fluid balance in the body and plays a critical role in maintaining heart function.

Pathways

ProBNP engages in the natriuretic peptide pathways and cyclic GMP signaling. It cleaves to generate BNP which interacts with NPR-A (natriuretic peptide receptor A) on the cell surface leading to increased production of cyclic GMP. This signaling further coordinates with essential proteins like ACE (angiotensin-converting enzyme) and angiotensin II which are fundamental in blood pressure regulation and cardiovascular function.

ProBNP levels correlate strongly with heart failure and acute coronary syndromes. Elevated proBNP or NT-proBNP often indicates stress on the heart due to volume overload or pressure signifying heart malfunction. BNP actively participates in counteracting the renin-angiotensin-aldosterone system (RAAS) helping mitigate the effects of heart failure when its function is not competing with excessive pathological levels of proteins like angiotensin II.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).

See full target information NPPB

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4345 PubMed35896549

2022

Targeting transcription in heart failure via CDK7/12/13 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Austin Hsu,Qiming Duan,Daniel S Day,Xin Luo,Sarah McMahon,Yu Huang,Zachary B Feldman,Zhen Jiang,Tinghu Zhang,Yanke Liang,Michael Alexanian,Arun Padmanabhan,Jonathan D Brown,Charles Y Lin,Nathanael S Gray,Richard A Young,Benoit G Bruneau,Saptarsi M Haldar

The Journal of clinical investigation 130:2966-2977 PubMed32106109

2020

Salt-inducible kinase 1 maintains HDAC7 stability to promote pathologic cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Austin Hsu,Qiming Duan,Sarah McMahon,Yu Huang,Sarah Ab Wood,Nathanael S Gray,Biao Wang,Benoit G Bruneau,Saptarsi M Haldar

Science translational medicine 9: PubMed28515341

2017

BET bromodomain inhibition suppresses innate inflammatory and profibrotic transcriptional networks in heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Duan,Sarah McMahon,Priti Anand,Hirsh Shah,Sean Thomas,Hazel T Salunga,Yu Huang,Rongli Zhang,Aarathi Sahadevan,Madeleine E Lemieux,Jonathan D Brown,Deepak Srivastava,James E Bradner,Timothy A McKinsey,Saptarsi M Haldar

Journal of biomolecular screening 18:1203-11 PubMed24071917

2013

Phenotypic screening with human iPS cell-derived cardiomyocytes: HTS-compatible assays for interrogating cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Coby Carlson,Chad Koonce,Natsuyo Aoyama,Shannon Einhorn,Steve Fiene,Arne Thompson,Brad Swanson,Blake Anson,Steven Kattman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-proBNP antibody [5B6]

ELISA - Anti-proBNP antibody [5B6] (AB13111)

Western blot - Anti-proBNP antibody [5B6] (AB13111)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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