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Mouse Monoclonal BNP antibody. Suitable for ELISA, WB and reacts with Synthetic peptide samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 1-50.

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Images

ELISA - Anti-proBNP antibody [5B6] (AB13111), expandable thumbnail
  • Western blot - Anti-proBNP antibody [5B6] (AB13111), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.1% Sodium azide

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human NPPB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P16860

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Synthetic peptide
Tested
Tested

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:1672777, PubMed:17372040, PubMed:1914098, PubMed:9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:1672777, PubMed:17349887, PubMed:17372040, PubMed:21098034, PubMed:25339504, PubMed:9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed:1914098, PubMed:9458824). Binds the clearance receptor NPR3 (PubMed:16870210). NT-proBNP. May affect cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed:17372040). BNP(3-32). May have a role in cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP (PubMed:17372040).

Alternative names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

Recommended products

Mouse Monoclonal BNP antibody. Suitable for ELISA, WB and reacts with Synthetic peptide samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 1-50.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human NPPB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P16860
Clone number
5B6
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from ascites to TCS on 10th June 2020.

Immunoglobulin concentrations vary from batch to batch (generally 4-6 mg/ml for this clone). Please contact technical services for the latest information.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The proBNP also known by names like NT-proBNP and brain natriuretic peptide precursor is a peptide that plays an important role as a cardiac biomarker. This protein has a molecular mass of about 13 kDa and is expressed primarily in cardiac tissues especially the ventricles. ProBNP is synthesized as a precursor and then processed to produce active BNP (B-type natriuretic peptide) and the inactive part NT-proBNP which can be used for diagnosis purposes.

Biological function summary

ProBNP contributes significantly to cardiovascular homeostasis by promoting natriuresis and diuresis which help in reducing blood pressure and volume. It does not form part of a larger protein complex but functions both in the formation of its active peptide BNP and its inactive fragment NT-proBNP. By exerting these effects proBNP influences fluid balance in the body and plays a critical role in maintaining heart function.

Pathways

ProBNP engages in the natriuretic peptide pathways and cyclic GMP signaling. It cleaves to generate BNP which interacts with NPR-A (natriuretic peptide receptor A) on the cell surface leading to increased production of cyclic GMP. This signaling further coordinates with essential proteins like ACE (angiotensin-converting enzyme) and angiotensin II which are fundamental in blood pressure regulation and cardiovascular function.

Associated diseases and disorders

ProBNP levels correlate strongly with heart failure and acute coronary syndromes. Elevated proBNP or NT-proBNP often indicates stress on the heart due to volume overload or pressure signifying heart malfunction. BNP actively participates in counteracting the renin-angiotensin-aldosterone system (RAAS) helping mitigate the effects of heart failure when its function is not competing with excessive pathological levels of proteins like angiotensin II.

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Product protocols

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