Mouse Monoclonal BNP antibody. Suitable for WB and reacts with Pig, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nppb aa 1-100 conjugated to Ovalbumin.
Images
![Western blot - Anti-proBNP antibody [C1] (AB239514), expandable thumbnail](https://content.abcam.com/products/images/probnp-antibody-c1-ab239514--western-blot-img31253.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-proBNP antibody [C1] (AB239514)")
![Western blot - Anti-proBNP antibody [C1] (AB239514), expandable thumbnail](https://content.abcam.com/products/images/probnp-antibody-c1-ab239514--western-blot-img159446.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-proBNP antibody [C1] (AB239514)")
Publications
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- Frontiers in endocrinology 13:10297502022Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinda Bartosova,Csaba Horvath,Peter Galis,Kristina Ferenczyova,Barbora Kalocayova,Adrian Szobi,Adriana Duris-Adameova,Monika Bartekova,Tomas RajtikPubMed 36568083
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Growth hormone-releasing hormone agonists ameliorate chronic kidney disease-induced heart failure with preserved ejection fraction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela C Rieger,Luiza L Bagno,Alessandro Salerno,Victoria Florea,Jose Rodriguez,Marcos Rosado,Darren Turner,Raul A Dulce,Lauro M Takeuchi,Rosemeire M Kanashiro-Takeuchi,Peter Buchwald,Amarylis C B A Wanschel,Wayne Balkan,Ivonne H Schulman,Andrew V Schally,Joshua M HarePubMed 33468654
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Mouse Nppb aa 1-100 conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information. Database link P40753
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Tested |
| Pig | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:1672777, PubMed:17372040, PubMed:1914098, PubMed:9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:1672777, PubMed:17349887, PubMed:17372040, PubMed:21098034, PubMed:25339504, PubMed:9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed:1914098, PubMed:9458824). Binds the clearance receptor NPR3 (PubMed:16870210). NT-proBNP. May affect cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed:17372040). BNP(3-32). May have a role in cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP (PubMed:17372040).
Alternative names
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB
Recommended products
Mouse Monoclonal BNP antibody. Suitable for WB and reacts with Pig, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nppb aa 1-100 conjugated to Ovalbumin.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Mouse Nppb aa 1-100 conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information. Database link P40753
- Clone number
- C1
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The proBNP also known by names like NT-proBNP and brain natriuretic peptide precursor is a peptide that plays an important role as a cardiac biomarker. This protein has a molecular mass of about 13 kDa and is expressed primarily in cardiac tissues especially the ventricles. ProBNP is synthesized as a precursor and then processed to produce active BNP (B-type natriuretic peptide) and the inactive part NT-proBNP which can be used for diagnosis purposes.
- Biological function summary
ProBNP contributes significantly to cardiovascular homeostasis by promoting natriuresis and diuresis which help in reducing blood pressure and volume. It does not form part of a larger protein complex but functions both in the formation of its active peptide BNP and its inactive fragment NT-proBNP. By exerting these effects proBNP influences fluid balance in the body and plays a critical role in maintaining heart function.
- Pathways
ProBNP engages in the natriuretic peptide pathways and cyclic GMP signaling. It cleaves to generate BNP which interacts with NPR-A (natriuretic peptide receptor A) on the cell surface leading to increased production of cyclic GMP. This signaling further coordinates with essential proteins like ACE (angiotensin-converting enzyme) and angiotensin II which are fundamental in blood pressure regulation and cardiovascular function.
- Associated diseases and disorders
ProBNP levels correlate strongly with heart failure and acute coronary syndromes. Elevated proBNP or NT-proBNP often indicates stress on the heart due to volume overload or pressure signifying heart malfunction. BNP actively participates in counteracting the renin-angiotensin-aldosterone system (RAAS) helping mitigate the effects of heart failure when its function is not competing with excessive pathological levels of proteins like angiotensin II.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Western blot - Anti-proBNP antibody [C1] (ab239514), expandable thumbnail](https://content.abcam.com/products/images/probnp-antibody-c1-ab239514--western-blot-img31253.jpg "Western blot - Anti-proBNP antibody [C1] (ab239514)")
Western blot - Anti-proBNP antibody [C1] (ab239514)
All lanes: Western blot - Anti-proBNP antibody [C1] (ab239514) at 3 µg/mL
Lane 1: Human serum
Lane 2: Rat heart tissue lysate
SecondaryAll lanes: HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 15 kDa
![Western blot - Anti-proBNP antibody [C1] (ab239514), expandable thumbnail](https://content.abcam.com/products/images/probnp-antibody-c1-ab239514--western-blot-img159446.jpg "Western blot - Anti-proBNP antibody [C1] (ab239514)")
Western blot - Anti-proBNP antibody [C1] (ab239514)
All lanes: Western blot - Anti-proBNP antibody [C1] (ab239514) at 3 µg/mL
All lanes: Pig heart lysate
SecondaryAll lanes: HRP-Goat Anti-Mouse IgG at 0.2 µg/mL
Predicted band size: 15 kDa
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