AB239514

Anti-proBNP antibody [C1]

Name: Anti-proBNP antibody [C1] (ab239514) | Abcam
Brand: Abcam Limited
SKU: AB239514
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal BNP antibody. Suitable for WB and reacts with Pig, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nppb aa 1-100 conjugated to Ovalbumin.

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

2 Images

Supplier Data

Western blot - Anti-proBNP antibody [C1] (AB239514)

All lanes:

Western blot - Anti-proBNP antibody [C1] (ab239514) at 3 µg/mL

Lane 1:

Human serum

Lane 2:

Rat heart tissue lysate

Secondary

All lanes:

HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-proBNP antibody [C1] (AB239514)

All lanes:

Western blot - Anti-proBNP antibody [C1] (ab239514) at 3 µg/mL

All lanes:

Pig heart lysate

Secondary

All lanes:

HRP-Goat Anti-Mouse IgG at 0.2 µg/mL

Predicted band size: 15 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Mouse Nppb aa 1-100 conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information.

P40753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proBNP also known by names like NT-proBNP and brain natriuretic peptide precursor is a peptide that plays an important role as a cardiac biomarker. This protein has a molecular mass of about 13 kDa and is expressed primarily in cardiac tissues especially the ventricles. ProBNP is synthesized as a precursor and then processed to produce active BNP (B-type natriuretic peptide) and the inactive part NT-proBNP which can be used for diagnosis purposes.

Biological function summary

ProBNP contributes significantly to cardiovascular homeostasis by promoting natriuresis and diuresis which help in reducing blood pressure and volume. It does not form part of a larger protein complex but functions both in the formation of its active peptide BNP and its inactive fragment NT-proBNP. By exerting these effects proBNP influences fluid balance in the body and plays a critical role in maintaining heart function.

Pathways

ProBNP engages in the natriuretic peptide pathways and cyclic GMP signaling. It cleaves to generate BNP which interacts with NPR-A (natriuretic peptide receptor A) on the cell surface leading to increased production of cyclic GMP. This signaling further coordinates with essential proteins like ACE (angiotensin-converting enzyme) and angiotensin II which are fundamental in blood pressure regulation and cardiovascular function.

ProBNP levels correlate strongly with heart failure and acute coronary syndromes. Elevated proBNP or NT-proBNP often indicates stress on the heart due to volume overload or pressure signifying heart malfunction. BNP actively participates in counteracting the renin-angiotensin-aldosterone system (RAAS) helping mitigate the effects of heart failure when its function is not competing with excessive pathological levels of proteins like angiotensin II.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).

See full target information NPPB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 15:3434-3448 PubMed40948531

2025

Differentiating early-stage nasopharyngeal carcinoma from adenoidal hypertrophy via SLC40A1 expression and developing a prognostic model for disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Wang,Suqing Qi,Chaobing Liu,Zhenhua Qiao,Chao Zhang

Frontiers in endocrinology 13:1029750 PubMed36568083

2022

Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Bartosova,Csaba Horvath,Peter Galis,Kristina Ferenczyova,Barbora Kalocayova,Adrian Szobi,Adriana Duris-Adameova,Monika Bartekova,Tomas Rajtik

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33468654

2021

Growth hormone-releasing hormone agonists ameliorate chronic kidney disease-induced heart failure with preserved ejection fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Angela C Rieger,Luiza L Bagno,Alessandro Salerno,Victoria Florea,Jose Rodriguez,Marcos Rosado,Darren Turner,Raul A Dulce,Lauro M Takeuchi,Rosemeire M Kanashiro-Takeuchi,Peter Buchwald,Amarylis C B A Wanschel,Wayne Balkan,Ivonne H Schulman,Andrew V Schally,Joshua M Hare

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-proBNP antibody [C1]

Western blot - Anti-proBNP antibody [C1] (AB239514)

Western blot - Anti-proBNP antibody [C1] (AB239514)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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