AB239519

Anti-proBNP antibody [C11]

Name: Anti-proBNP antibody [C11] (ab239519) | Abcam
Brand: Abcam Limited
SKU: AB239519
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal BNP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NPPB aa 100 to C-terminus conjugated to Ovalbumin.

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, NPPB

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Formalin-fixed, paraffin-embedded human liver tissue stained for proBNP with ab239519 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Formalin-fixed, paraffin-embedded human glioma tissue stained for proBNP with ab239519 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for proBNP with ab239519 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Formalin-fixed, paraffin-embedded human stomach tissue stained for proBNP with ab239519 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C11

Isotype

IgG2b

Light chain type

lambda

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NPPB aa 100 to C-terminus conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information.

P16860

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proBNP also known by names like NT-proBNP and brain natriuretic peptide precursor is a peptide that plays an important role as a cardiac biomarker. This protein has a molecular mass of about 13 kDa and is expressed primarily in cardiac tissues especially the ventricles. ProBNP is synthesized as a precursor and then processed to produce active BNP (B-type natriuretic peptide) and the inactive part NT-proBNP which can be used for diagnosis purposes.

Biological function summary

ProBNP contributes significantly to cardiovascular homeostasis by promoting natriuresis and diuresis which help in reducing blood pressure and volume. It does not form part of a larger protein complex but functions both in the formation of its active peptide BNP and its inactive fragment NT-proBNP. By exerting these effects proBNP influences fluid balance in the body and plays a critical role in maintaining heart function.

Pathways

ProBNP engages in the natriuretic peptide pathways and cyclic GMP signaling. It cleaves to generate BNP which interacts with NPR-A (natriuretic peptide receptor A) on the cell surface leading to increased production of cyclic GMP. This signaling further coordinates with essential proteins like ACE (angiotensin-converting enzyme) and angiotensin II which are fundamental in blood pressure regulation and cardiovascular function.

ProBNP levels correlate strongly with heart failure and acute coronary syndromes. Elevated proBNP or NT-proBNP often indicates stress on the heart due to volume overload or pressure signifying heart malfunction. BNP actively participates in counteracting the renin-angiotensin-aldosterone system (RAAS) helping mitigate the effects of heart failure when its function is not competing with excessive pathological levels of proteins like angiotensin II.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Brain natriuretic peptide 32. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 1672777, PubMed : 17372040, PubMed : 1914098, PubMed : 9458824). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed : 1672777, PubMed : 17349887, PubMed : 17372040, PubMed : 21098034, PubMed : 25339504, PubMed : 9458824). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed : 1914098, PubMed : 9458824). Binds the clearance receptor NPR3 (PubMed : 16870210).. NT-proBNP. May affect cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed : 17372040).. BNP(3-32). May have a role in cardio-renal homeostasis (PubMed : 17372040). Able to promote the production of cGMP (PubMed : 17372040).

See full target information NPPB

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular research 120:2163-2165 PubMed39238367

2024

Identifying optimal reference genes for real-time quantitative polymerase chain reaction in human myocardial tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Camacho-Encina,Laura K Booth,Rachael Redgrave,Minna Honkanen-Scott,William E Scott,Carmen Martin-Ruiz,Guy MacGowan,Sarah Richardson,John Dark,Simon Tual-Chalot,Gavin D Richardson

Journal of biochemical and molecular toxicology 36:e23025 PubMed35603999

2022

NF-κB-upregulated miR-155-5p promotes hepatocyte mitochondrial dysfunction to accelerate the development of nonalcoholic fatty liver disease through downregulation of STC1.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Shen,Hanghai Pan,Jinjing Ke,Fei Zhao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-proBNP antibody [C11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (AB239519)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise