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Rat Recombinant Monoclonal Calcitonin antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] (AB252821), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (AB252821), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (AB252821), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (AB252821), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed:35324283). Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.

Alternative names

CALC1, CALCA, Calcitonin

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Rat Recombinant Monoclonal Calcitonin antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
4A6
Purification technique
Ion exchange chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Procalcitonin also known as PCT is a protein precursor of the hormone calcitonin. It has a molecular mass of about 13 kilodaltons. Procalcitonin is expressed in the thyroid gland typically but can also be produced in other tissues like the liver and kidney during inflammation or sepsis. It gets increasingly produced in response to bacterial infections making it a significant marker for conditions involving systemic inflammation.

Biological function summary

The function of procalcitonin is related to its precursor status for calcitonin which helps regulate calcium homeostasis. During infections PCT levels rise without its cleavage into active calcitonin indicating that its biological role extends beyond calcium regulation. It does not form part of any larger protein complex in its marker role. When released in response to bacterial infections procalcitonin serves as an indicator for the severity of the infection which is particularly useful in distinguishing bacterial from viral infections.

Pathways

Procalcitonin gets involved in the immune response pathway primarily due to its expression during bacterial infections. An important pathway is the inflammatory response where procalcitonin production gets triggered serving as a mediator in immune system activation. It is related to cytokines in the immune response which elevated PCT can represent indirectly indicating the presence and level of infection-driven inflammation.

Associated diseases and disorders

Procalcitonin levels are used significantly in diagnosing and managing sepsis and bacterial infections. Procalcitonin concentrations rise sharply in a bacterial systemic infection and help differentiate it from viral infections an important diagnostic criterion. Additionally in sepsis another important protein involved is C-reactive protein (CRP) often used alongside PCT as a biomarker for inflammatory states in the body. This relationship underlines the importance of PCT in managing infectious diseases through targeted diagnostic pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] (ab252821), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT cells labelling Procalcitonin with ab252821 at 1/500 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in TT cells. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunohistochemical analysis of paraffin-embedded human thyroid medullary carcinoma tissue labeling Procalcitonin with ab252821 at 1/1000 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on human thyroid medullary carcinoma. The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Procalcitonin with ab252821 at 1/100 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Negative control: No staining on human kidney.

    The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunohistochemical analysis of paraffin-embedded human follicular adenocarcinoma of thyroid tissue labeling Procalcitonin with ab252821 at 1/1000 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on human follicular adenocarcinoma of thyroid. The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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