Anti-proCathepsin D antibody [EPR3054] - BSA and Azide free

• WB

Lab

Western blot - Anti-proCathepsin D antibody [EPR3054] - BSA and Azide free (AB206472)

Lanes 1 - 4 : Merged signal (red and green). Green - ab134169 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab134169 was shown to recognize in wild-type MCF7 cells as signal was lost at the expected MW in HepG2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HepG2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab134169 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134169).

All lanes:

Western blot - Anti-proCathepsin D antibody [EPR3054] (<a href='/en-us/products/primary-antibodies/procathepsin-d-antibody-epr3054-ab134169'>ab134169</a>) at 1/2000 dilution

Lane 1:

Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 40 µg

Lane 2:

Cathepsin D knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 40 µg

Lane 2:

Western blot - Human CTSD knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-ctsd-knockout-a-431-cell-line-ab261891'>ab261891</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 40 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 40 µg

Predicted band size: 44 kDa,45 kDa

Observed band size: 46 kDa

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