AB240043

Anti-proCNP antibody [EPR6543] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal proCNP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CNP2, NPPC, C-type natriuretic peptide

1 Images

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Western blot - Anti-proCNP antibody [EPR6543] - BSA and Azide free (AB240043)

This data was developed using ab129096, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-proCNP antibody [EPR6543] (<a href='/en-us/products/primary-antibodies/procnp-antibody-epr6543-ab129096'>ab129096</a>) at 1/10000 dilution

All lanes:

Human proCNP recombinant protein at 0.01 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 13 kDa

Observed band size: 6 kDa

false

Unconjugated

Anti-proCNP antibody [EPR6543]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6543

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "NOTE: ab240043 has only been tested against the recombinant protein.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240043 is the carrier-free version of ab129096.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ProCNP also known as pro-C-type natriuretic peptide is an inactive precursor to C-type natriuretic peptide (CNP) a member of the natriuretic peptide family. ProCNP has a molecular mass of approximately 12 kDa. This peptide is particularly expressed in the central nervous system bones and blood vessels. CNP is widely distributed in brain tissues and offers significant biological effects in these areas which are contrasted to the limited distribution and distinct functions of the other natriuretic peptides.

Biological function summary

Pro-C-type natriuretic peptide plays an important role in the regulation of vascular tone and bone growth. It does not act on its own but must be processed into active CNP to exert its functions. CNP does not work as part of a complex but directly interacts with particulate guanylyl cyclase membrane receptor-B (NPR-B) which mediates its effects on target tissues by increasing cyclic guanosine monophosphate (cGMP) levels.

Pathways

ProCNP influences two main pathways: the natriuretic peptide signaling pathway and bone development pathways. In particular CNP derived from proCNP regulates endochondral ossification and promotes chondrocyte differentiation. This regulation is essential in growth plate development and bone lengthening. CNP associated with natriuretic peptide receptor B contrasts with atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) which more intensely modulate cardiovascular functions.

ProCNP when processed to CNP demonstrates a link to achondroplasia a genetic disorder of bone growth leading to dwarfism. Alterations in the CNP-NPR-B pathway due to genetic mutations can impair normal bone development. Moreover it has an association with cardiovascular conditions where abnormal levels affect vascular homeostasis. These effects relate to the interaction with other natriuretic peptides like BNP as studies frequently measure proCNP levels alongside BNP in heart-related assessments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CNP-22. Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation (By similarity). May also be vasoactive and natriuretic (PubMed : 1672777). Acts by specifically binding and stimulating NPR2 to produce cGMP (PubMed : 1672777, PubMed : 21098034). Binds the clearance receptor NPR3 (PubMed : 11533490).

See full target information NPPC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 9:1055356 PubMed36518851

2022

The copper transporter CTR1 and cisplatin accumulation at the single-cell level by LA-ICP-TOFMS.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Schoeberl,Michael Gutmann,Sarah Theiner,Mario Corte-Rodríguez,Gabriel Braun,Petra Vician,Walter Berger,Gunda Koellensperger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-proCNP antibody [EPR6543] - BSA and Azide free

Western blot - Anti-proCNP antibody [EPR6543] - BSA and Azide free (AB240043)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Assay Kits

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Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise