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AB285003

Anti-Procollagen III N-terminal propeptide (PIIINP) antibody [EPR24257-178] - BSA and Azide free (Capture)

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(2 Publications)

Rabbit Recombinant Monoclonal Collagen III antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Collagen alpha-1(III) chain, COL3A1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24257-178

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab285003 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Collagen III. The recommended pair for sandwich ELISA is:
     Capture: ab285003, Mouse Procollagen III N-terminal propeptide (PIIINP) Capture Antibody (unconjugated)
     Detector: ab285109, Mouse Procollagen III N-terminal propeptide (PIIINP) Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen III N-terminal propeptide known as PIIINP serves as an important marker in collagen synthesis processes. PIIINP results from the cleavage of procollagen III and functions as a byproduct of the maturation of collagen molecules. It possesses a molecular weight of approximately 42 kDa. This target is initially expressed within fibroblast cells found in connective tissues and then secreted into the bloodstream. Given its role in collagen synthesis it is frequently measured to assess the turnover and formation of collagen in tissues.
Biological function summary

This propeptide plays a significant role in the formation of the extracellular matrix. PIIINP is not part of a protein complex but accumulates during the conversion of procollagen into mature collagen fibers which later support tissue structure. Its presence is essential for proper collagen deposition and tissue remodeling. Through its involvement in collagen synthesis PIIINP helps maintain the structural integrity of connective tissues across various organs.

Pathways

Its role reflects the broader functions of collagen metabolism and tissue remodeling pathways. PIIINP marks the processing pathway of procollagen to collagen which is important for the formation of the extracellular matrix. This pathway sometimes intersects with pathways involving other collagens such as collagen I which is another critical component of connective tissues. Together PIIINP and collagen I contribute to the proper maintenance of the extracellular matrix and the mechanical properties of tissues.

Elevated levels of PIIINP often relate to fibrotic conditions and can serve as a biomarker for diseases like liver fibrosis and systemic sclerosis. In liver fibrosis excessive collagen deposition and remodeling occur affecting liver function. In systemic sclerosis abnormal collagen synthesis and deposition in the skin cause thickening and hardening. PIIINP connection to these conditions often involves collagen-binding proteins which regulate collagen stability and deposition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information COL3A1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 8:e10480 PubMed36105473

2022

Experimental study on the effects of simvastatin in reversing the femoral metaphyseal defects induced by sodium valproate in normal and ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Ruotian Zhang,Maoxian Ren,Hedong Liu,Min Yang

Redox biology 56:102435 PubMed36029649

2022

Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Li,Kunpeng Wang,Rongjun Jia,Jing Xie,Liman Ma,Zhiqing Hao,Weiwei Zhang,Jinggang Mo,Fu Ren
View all publications
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Product promise

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