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AB203875

Anti-PRODH antibody

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(4 Publications)

Rabbit Polyclonal PRODH antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PRODH aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PIG6, POX2, PRODH2, PRODH, Proline oxidase, Proline oxidase 2, p53-induced gene 6 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH antibody (AB203875)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH antibody (AB203875)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse kidney tissue, labeling PRODH using ab203875 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH antibody (AB203875)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH antibody (AB203875)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PRODH using ab203875 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PRODH aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43272

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe please use dilutions of 1/50-1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe please use dilutions of 1/50-1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRODH also known as proline dehydrogenase or proline oxidase is an enzyme that plays an important mechanical role in proline metabolism. This mitochondrial protein with a mass of approximately 64 kDa catalyzes the conversion of proline to pyrroline-5-carboxylate a step important in proline degradation. PRODH is mainly expressed in several tissues including the brain heart and kidney which suggests its importance in various physiological processes.
Biological function summary

PRODH contributes to cellular energy production and redox balance by participating in proline catabolism. It operates as part of the proline utilization pathway carrying out key reactions necessary for maintaining amino acid balance and energy homeostasis. Although PRODH functions independently it collaborates with other enzymes in the mitochondrial matrix to facilitate efficient energy production and metabolic regulation.

Pathways

PRODH is involved in proline and arginine metabolism which intersects with the urea cycle and glutamate metabolism. Its activity impinges on the regulation of key pathways like the Krebs cycle through its product pyrroline-5-carboxylate which further converts to glutamate. In this context PRODH is functionally related to other enzymes such as pyrroline-5-carboxylate reductase which modulates the flow and conversion of metabolites within these pathways.

Researchers have linked PRODH to disorders like schizophrenia and cancer. Altered expression or mutations in the PRODH gene can impact metabolic flux in the brain contributing to neuropsychiatric conditions such as schizophrenia. Also the enzyme's role in proline metabolism connects it to tumorogenesis where shifts in metabolic pathways support cancer cell growth. PRODH's interactions with proteins like p53 suggest a potential mechanism for its involvement in oncogenic processes highlighting its significance in disease progression and therapeutic target potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts proline to delta-1-pyrroline-5-carboxylate.
See full target information PRODH
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:102820 PubMed36549646

2022

Smad2Δexon3 and Smad3 have distinct properties in signal transmission leading to TGF-β-induced cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yokoyama,Takahito Kuga,Yuka Itoh,Shigeo Otake,Chiho Omata,Masao Saitoh,Keiji Miyazawa

Biomolecules 11: PubMed34067570

2021

L-Proline Activates Mammalian Target of Rapamycin Complex 1 and Modulates Redox Environment in Porcine Trophectoderm Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Liu,Ying Yang,Xuemeng Si,Hai Jia,Yunchang Zhang,Da Jiang,Zhaolai Dai,Zhenlong Wu

Journal of molecular cell biology : PubMed33508123

2021

OIP5-AS1 specifies p53-driven POX transcription regulated by TRPC6 in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Shao,Zhen-Yu Hao,Yi-Fei Chen,Jun Du,Qian He,Liang-Liang Ren,Yan Gao,Nan Song,Yan Song,Hua He,Yi-Zheng Wang

Aging cell 20:e13308 PubMed33480139

2021

Incomplete proline catabolism drives premature sperm aging.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-An Yen,Sean P Curran
View all publications
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