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AB151130

Anti-PRODH2 antibody

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(2 Publications)

Rabbit Polyclonal PRODH2 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRODH2 aa 200-300.

View Alternative Names

HSPOX1, HYPDH, PRODH2, Hydroxyproline dehydrogenase, Kidney and liver proline oxidase 1, Probable proline dehydrogenase 2, Probable proline oxidase 2, HsPOX1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH2 antibody (AB151130)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH2 antibody (AB151130)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRODH2 with ab151130 at 1/500 dilution.

Immunocytochemistry - Anti-PRODH2 antibody (AB151130)
  • ICC

Supplier Data

Immunocytochemistry - Anti-PRODH2 antibody (AB151130)

HepG2 cells stained for PRODH2 (green) using ab151130 at 2 μg/ml dilution in ICC/IF.

Western blot - Anti-PRODH2 antibody (AB151130)
  • WB

Supplier Data

Western blot - Anti-PRODH2 antibody (AB151130)

All lanes:

Western blot - Anti-PRODH2 antibody (ab151130) at 0.4 µg/mL

Lane 1:

RT4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver tissue lysate

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human PRODH2 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UF12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRODH2 also known as proline dehydrogenase is an enzyme with a molecular mass of approximately 65 kDa. It functions as a catalyst in the oxidation of proline into pyrroline-5-carboxylate. This enzyme facilitates the first step in the proline degradation pathway. It is a mitochondrial protein widely expressed in various tissues with higher levels detected in the brain liver and kidney.
Biological function summary

PRODH2 is essential for amino acid metabolism particularly in proline catabolism. This enzyme helps regulate the balance of proline levels within cells which is important for cellular homeostasis. Although PRODH2 operates largely as an individual unit it may interact with other enzymes within mitochondrial pathways. This interaction supports its role in producing necessary cellular energy and intermediates.

Pathways

PRODH2 plays an important role in mitochondrial respiration and the interconnected network of amino acid metabolism. It is closely linked with the proline biosynthesis and degradation pathway. This pathway also involves the enzyme pyrroline-5-carboxylate reductase which works in tandem with PRODH2 to maintain amino acid turnover and energy production in the mitochondria.

Alterations in PRODH2 function have been associated with schizophrenia and hyperprolinemia. The former is a neuropsychiatric disorder where PRODH2’s altered activity may disrupt normal brain metabolism. In hyperprolinemia a deficiency or malfunction in PRODH2 leads to an accumulation of proline causing various metabolic complications. Schizophrenia also involves interactions with other proteins like DISC1 which influence neural pathways and can link to metabolic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. Can also use proline as a substrate but with a very much lower efficiency. Does not react with other diastereomers of Hyp : trans-4-D-hydroxyproline and cis-4-L-hydroxyproline. Ubiquininone analogs such as menadione, duroquinone and ubiquinone-1 react more efficiently than oxygen as the terminal electron acceptor during catalysis.
See full target information PRODH2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:318 PubMed37169762

2023

Single-cell sequencing reveals homogeneity and heterogeneity of the cytopathological mechanisms in different etiology-induced AKI.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Chen,Yinshuang Li,Ying Yuan,Kunmei Lai,Keng Ye,Yujiao Lin,Ruilong Lan,Hong Chen,Yanfang Xu

Nature metabolism 3:571-585 PubMed33833463

2021

Mitochondrial NADP is essential for proline biosynthesis during cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Diem H Tran,Rushendhiran Kesavan,Halie Rion,Mona Hoseini Soflaee,Ashley Solmonson,Divya Bezwada,Hieu S Vu,Feng Cai,John A Phillips,Ralph J DeBerardinis,Gerta Hoxhaj
View all publications
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