Rabbit Recombinant Monoclonal ProDynorphin antibody. Carrier free. Suitable for mIHC, Dot, WB, IP, IHC-Fr, IHC-P and reacts with Mouse, Rat, Synthetic peptide - Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (AB318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433377.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (AB318967)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (AB318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433380.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (AB318967)")
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (AB318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img435793.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (AB318967)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | Dot | WB | IP | IHC-Fr | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Expected | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Expected | Expected | Tested | Expected | Tested | Tested | Not recommended |
| Synthetic peptide - Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
Target data
Function
Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (By similarity).Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opioid activity, it is 700 times more potent than Leu-enkephalin (By similarity).Leumorphin has a typical opioid activity and may have anti-apoptotic effect.
Alternative names
Proenkephalin-B, Beta-neoendorphin-dynorphin, Preprodynorphin
Recommended products
Rabbit Recombinant Monoclonal ProDynorphin antibody. Carrier free. Suitable for mIHC, Dot, WB, IP, IHC-Fr, IHC-P and reacts with Mouse, Rat, Synthetic peptide - Mouse samples.
Alternative names
Proenkephalin-B, Beta-neoendorphin-dynorphin, Preprodynorphin
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28632-48
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab318967 is the carrier-free version of Anti-ProDynorphin antibody [EPR28632-48] ab318966.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ProDynorphin also known as PDYN or preproenkephalin B serves as a precursor protein for the dynorphins. Dynorphins are class of opioid peptides that the body uses for modulating pain response and tailored hormone release. ProDynorphin appears with a mass of around 25 kDa and is expressed widely in the brain including areas such as the hippocampus and hypothalamus. Localization in the pituitary gland also occurs where it significantly contributes to the regulation of neuroendocrine systems.
- Biological function summary
This protein influences a variety of physiological processes. Neurons express ProDynorphin in abundant amounts reflecting its importance in neurotransmission where it acts through specific receptors like the kappa opioid receptor. ProDynorphin is not known to function as part of a larger multiprotein complex but its role in signal pathways clearly impacts pain perception emotional response and addiction mechanisms in the central nervous system by releasing dynorphin A peptides among others.
- Pathways
Researchers link ProDynorphin to significant opioid receptor pathways and stress response mechanisms. The protein interacts with key molecules such as the kappa opioid receptor influencing the opioid receptor signaling pathway. Another pathway influenced by ProDynorphin involves the dopamine system which connects it through shared roles with the peptide proenkephalin leading to potential impacts on reward and mood regulations due to dopamine's activity in these contexts.
- Associated diseases and disorders
ProDynorphin holds significance in conditions like chronic pain and mood disorders. Dysregulation or mutation in the gene encoding this protein associates with altered pain sensitivity and susceptibility to depressive disorders. Its direct connection to the kappa opioid receptor further ties it to addiction-related disorders as kappa receptor signaling commonly impacts withdrawal and relapse behaviors. The study of ProDynorphin within these diseases offers potential therapeutic targets for managing pain and mood dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
19 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433377.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pituitary tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse pituitary.
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433380.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat pituitary.
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img435793.jpg "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat dorsomedial hypothalamic nucleus (perfused fixed) tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Confocal image showing positive staining on rat dorsomedial hypothalamic nucleus (DMH). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Immunohistochemistry (Frozen sections)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-ProDynorphin (Anti-ProDynorphin antibody [EPR28632-48] ab318966, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on rat hippocampus.
Panel B: anti-ProDynorphin stained on rat hippocampus.
Panel C: anti-NeuN stained in neurons of rat hippocampus.
Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
The section was incubated in two rounds of staining: in the order of Anti-ProDynorphin antibody [EPR28632-48] ab318966 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-ProDynorphin (Anti-ProDynorphin antibody [EPR28632-48] ab318966, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on mouse hippocampus.
Panel B: anti-ProDynorphin stained on mouse hippocampus.
Panel C: anti-NeuN stained in neurons of mouse hippocampus.
Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
The section was incubated in two rounds of staining: in the order of Anti-ProDynorphin antibody [EPR28632-48] ab318966 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img435794.jpg "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse arcuate nucleus (perfused fixed) tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Confocal image showing positive staining on mouse arcuate nucleus (ARC) of hypothalamus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
![Multiplex immunohistochemistry - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--multiplex-immunohistochemistry-img434186.jpg "Multiplex immunohistochemistry - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Multiplex immunohistochemistry - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ProDynorphin antibody [EPR28632-48] ab318966).
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse hippocampus tissue staining ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at a 1:5000 (0.1 ug/ml) dilution, SYNPR with Anti-SYNPR antibody [EPR28139-74] ab314136 at 1:8000 (0.06 ug/ml) dilution and Wolframin with Anti-WFS1 antibody [EPR23801-91] ab259362 at a 1:5000 (0.091 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-ProDynorphin (magenta; Opal™570), anti-SYNPR (green; Opal™520) and anti-Wolframin (yellow; Opal™690) on mouse hippocampus.
Panel B: anti-ProDynorphin staining the mossy fibers of CA3 in mouse hippocampus.
Panel C: anti-SYNPR staining the mossy fibers of CA3 in mouse hippocampus.
Panel D: anti-Wolframin staining the pyramidal neurons of CA1 in mouse hippocampus.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-ProDynorphin antibody [EPR28632-48] ab318966, Anti-SYNPR antibody [EPR28139-74] ab314136 and Anti-WFS1 antibody [EPR23801-91] ab259362 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--western-blot-img433345.jpg "Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Negative control: liver(PMID: 3864164), kidney.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16966485, PMID: 9712897).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ProDynorphin antibody [EPR28632-48] (Anti-ProDynorphin antibody [EPR28632-48] ab318966) at 1/1000 dilution
Lane 1: Mouse pituitary tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 30 µg
Lane 3: Mouse liver tissue lysate at 30 µg
Lane 4: Rat pituitary tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 30 µg
Lane 6: Rat kidney tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 18 kDa, 28 kDa, 36 kDa
Exposure time: 10s
Negative control: liver(PMID: 3864164), kidney.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16966485, PMID: 9712897).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433381.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse liver.
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433378.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse hippocampus.
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Dot Blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--dot-blot-img433422.jpg "Dot Blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Dot Blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Dot blot analysis of ProDynorphin using Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1:1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
All lanes: Dot Blot - Anti-ProDynorphin antibody [EPR28632-48] (Anti-ProDynorphin antibody [EPR28632-48] ab318966) at 1/1000 dilution
Lane 1: Mouse Proenkephalin-B full-length protein
Lane 2: Mouse Proenkephalin-B pro-peptide
Lane 3: Mouse Proenkephalin-B (Dynorphin A) peptide
Lane 4: Mouse Proenkephalin-B (Alpha-neoendorphin) peptide
Lane 5: Mouse Proenkephalin-B (Big dynorphin) peptide
Lane 6: Mouse Proenkephalin-B peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Dot blot analysis of ProDynorphin using Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1:1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433376.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse striatum.
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433379.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat striatum.
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img433414.jpg "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal ima ge showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunoprecipitation - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunoprecipitation-img433394.jpg "Immunoprecipitation - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunoprecipitation - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
ProDynorphin was immunoprecipitated from 0.35 mg Mouse pituitary tissue lysate with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse pituitary tissue lysate
Lane 2: Anti-ProDynorphin antibody [EPR28632-48] ab318966 IP in Mouse pituitary tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ProDynorphin antibody [EPR28632-48] ab318966 in mouse pituitary tissue lysateAll lanes: Immunoprecipitation - Anti-ProDynorphin antibody [EPR28632-48] (Anti-ProDynorphin antibody [EPR28632-48] ab318966) at 1/30 dilution
All lanes: Mouse pituitary tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
![Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--western-blot-img433347.jpg "Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Low expression: cerebellum (PMID: 16966485).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16966485, PMID: 9712897).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ProDynorphin antibody [EPR28632-48] (Anti-ProDynorphin antibody [EPR28632-48] ab318966) at 1/1000 dilution
Lane 1: Rat striatum tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Lane 3: Rat cerebellum tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 18 kDa, 28 kDa, 36 kDa
Exposure time: 37s
Low expression: cerebellum (PMID: 16966485).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16966485, PMID: 9712897).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--western-blot-img433346.jpg "Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Low expression: cerebellum (PMID: 16966485).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16966485, PMID: 9712897).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ProDynorphin antibody [EPR28632-48] (Anti-ProDynorphin antibody [EPR28632-48] ab318966) at 1/1000 dilution
Lane 1: Mouse striatum tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse cerebellum tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 18 kDa, 28 kDa, 36 kDa
Exposure time: 81s
Low expression: cerebellum (PMID: 16966485).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16966485, PMID: 9712897).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img433416.jpg "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/50 (10.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal ima ge showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433382.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)
This data was developed using Anti-ProDynorphin antibody [EPR28632-48] ab318966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ProDynorphin with Anti-ProDynorphin antibody [EPR28632-48] ab318966 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in rat liver (PMID: 3864164).
The section was incubated with Anti-ProDynorphin antibody [EPR28632-48] ab318966 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img435794.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Multiplex immunohistochemistry - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--multiplex-immunohistochemistry-img434186.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--western-blot-img433345.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433381.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433378.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Dot Blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--dot-blot-img433422.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433376.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433379.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img433414.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunoprecipitation - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunoprecipitation-img433394.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--western-blot-img433347.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--western-blot-img433346.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-frozen-sections-img433416.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967), expandable thumbnail](https://content.abcam.com/products/images/prodynorphin-antibody-epr28632-48-bsa-and-azide-free-ab318967--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433382.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ProDynorphin antibody [EPR28632-48] - BSA and Azide free (ab318967)")
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