Rabbit Recombinant Monoclonal ProDynorphin antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170194.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501)")
![Immunoprecipitation - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunoprecipitation-img173226.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501)")
![Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--western-blot-img180263.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501)")
![Dot Blot - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--dot-blot-img169137.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501)")
![Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--western-blot-img178001.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (AB283501)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Dot | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Tested | Tested | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat mediated antigen retrieval with sodium citrate buffer at pH 6.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat mediated antigen retrieval with sodium citrate buffer at pH 6.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (By similarity). Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opioid activity, it is 700 times more potent than Leu-enkephalin (By similarity). Leumorphin has a typical opioid activity and may have anti-apoptotic effect.
Alternative names
Proenkephalin-B, Beta-neoendorphin-dynorphin, Preprodynorphin, Pdyn
Recommended products
Rabbit Recombinant Monoclonal ProDynorphin antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24508-78
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ProDynorphin also known as PDYN or preproenkephalin B serves as a precursor protein for the dynorphins. Dynorphins are class of opioid peptides that the body uses for modulating pain response and tailored hormone release. ProDynorphin appears with a mass of around 25 kDa and is expressed widely in the brain including areas such as the hippocampus and hypothalamus. Localization in the pituitary gland also occurs where it significantly contributes to the regulation of neuroendocrine systems.
- Biological function summary
This protein influences a variety of physiological processes. Neurons express ProDynorphin in abundant amounts reflecting its importance in neurotransmission where it acts through specific receptors like the kappa opioid receptor. ProDynorphin is not known to function as part of a larger multiprotein complex but its role in signal pathways clearly impacts pain perception emotional response and addiction mechanisms in the central nervous system by releasing dynorphin A peptides among others.
- Pathways
Researchers link ProDynorphin to significant opioid receptor pathways and stress response mechanisms. The protein interacts with key molecules such as the kappa opioid receptor influencing the opioid receptor signaling pathway. Another pathway influenced by ProDynorphin involves the dopamine system which connects it through shared roles with the peptide proenkephalin leading to potential impacts on reward and mood regulations due to dopamine's activity in these contexts.
- Associated diseases and disorders
ProDynorphin holds significance in conditions like chronic pain and mood disorders. Dysregulation or mutation in the gene encoding this protein associates with altered pain sensitivity and susceptibility to depressive disorders. Its direct connection to the kappa opioid receptor further ties it to addiction-related disorders as kappa receptor signaling commonly impacts withdrawal and relapse behaviors. The study of ProDynorphin within these diseases offers potential therapeutic targets for managing pain and mood dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170194.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labelling ProDynorphin with ab283501 at 1/4000 (0.137 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat hippocampus (PMID: 16966485). The section was incubated with ab283501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunoprecipitation-img173226.jpg "Immunoprecipitation - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Immunoprecipitation - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
ProDynorphin was immunoprecipitated from 0.35 mg Rat pituitary tissue lysate with ab283501 at 1/30 dilution (2 ug in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab283501 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat pituitary tissue lysate 10 ug
Lane 2: ab283501 IP in Rat pituitary tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283501 in rat pituitary tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Predicted band size: 28 kDa
Observed band size: 18 kDa, 23 kDa, 28 kDa
![Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--western-blot-img180263.jpg "Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 9712897).
Negative control: Rat liver (PMID:23649822).
Exposure time: 26 seconds.
All lanes: Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501) at 1/1000 dilution
Lane 1: Rat pituitary tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 18 kDa, 23 kDa, 28 kDa
![Dot Blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--dot-blot-img169137.jpg "Dot Blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Dot Blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Dot blot analysis of ProDynorphin using ab283501 at 1/1000 (0.583 ug/ml) diultion followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution.
Exposure time: 3 minutes.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
![Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--western-blot-img178001.jpg "Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Western blot - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501) at 1/1000 dilution
Lane 1: LIF/His-rat ProDynorphin full-length protein at 0.01 µg
Lane 2: His-tagged rat ProDynorphin pro-peptide at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 18 kDa, 64 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167373.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labelling ProDynorphin with ab283501 at 1/4000 (0.137 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat pituitary (PMID: 16966485). The section was incubated with ab283501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-frozen-sections-img166596.jpg "Immunohistochemistry (Frozen sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Immunohistochemistry (Frozen sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling ProDynorphin with ab283501 at 1/100 (5.47 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution (Green). No staining on rat liver.Negative control: liver (PMID: 3864164) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/ml dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165542.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling ProDynorphin with ab283501 at 1/4000 (0.137 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on rat liver (PMID 23649822). The section was incubated with ab283501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167373.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
![Immunohistochemistry (Frozen sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-frozen-sections-img166596.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501), expandable thumbnail](https://content.abcam.com/products/images/proenkephalin-b-antibody-epr24508-78-ab283501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165542.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501)")
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