AB50667

Anti-Profilin 1 antibody - Carboxyterminal end

Name: Anti-Profilin 1 antibody - Carboxyterminal end (ab50667) | Abcam
Brand: Abcam Limited
SKU: AB50667
Rating: 5 (4 reviews)

Lab Essentials
What is this?

(4 Reviews)

(15 Publications)

Rabbit Polyclonal Profilin 1 antibody. Suitable for IP and reacts with Mouse samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PFN1 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Profilin-1, Epididymis tissue protein Li 184a, Profilin I, PFN1

1 Images

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Immunoprecipitation - Anti-Profilin 1 antibody - Carboxyterminal end (AB50667)

Lane 1 : Positve control — Western blot : ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.

Lane 2 : Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody : Rabbit Anti-Profilin 1 (N-terminal) antibody.

Lane 3 : Negative control — non-relevant antibody was used for IP. Detection antibody : Rabbit Anti-Profilin 1 (N-terminal) antibody

All lanes:

Immunoprecipitation - Anti-Profilin 1 antibody - Carboxyterminal end (ab50667)

Predicted band size: 15 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human PFN1 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07737

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Profilin 1 also known as Profilin-1 or Profilin is a small actin-binding protein with a molecular weight of approximately 15 kDa. It is expressed across various tissues including muscle brain and epithelial cells. Profilin 1 interacts with monomeric actin (G-actin) to regulate actin polymerization by binding to actin monomers. This protein also interacts with polyphosphoinositides and proline-rich sequences playing a mechanical role in balancing actin filament assembly and disassembly.

Biological function summary

Profilin 1 acts as an important regulator of the cytoskeleton facilitating cellular processes like motility and division. It participates in the formation of actin filaments by delivering actin monomers to barbed ends promoting polymerization. Profilin 1 is part of the complex that controls actin dynamics and this ability to modulate actin structure allows it to influence cell shape and internal movement. The protein's interactions help support the actin cytoskeleton for intracellular trafficking and signal transduction.

Pathways

Profilin 1 has important roles in the regulation of the actin cytoskeleton and Rho signaling pathways. These pathways are important in cell migration and adhesion. During cellular processes Profilin 1's interactions with actin and polyphosphoinositides link it to other proteins such as Rho GTPases which are associated with cytoskeletal dynamics. Profilin 1 can influence these pathways by modulating actin dynamics and interacting with proteins involved in these signaling cascades.

Disruptions in Profilin 1 function are linked to neurodegenerative diseases and cancer metastasis. For example aberrant Profilin 1 activity can contribute to amyotrophic lateral sclerosis (ALS) by affecting neuronal structure and transport. Dysregulation of its interaction with actin or polyphosphoinositides might also lead to enhanced cancer cell migration and invasion. It may interact with other proteins implicated in these diseases such as the ALS-related protein TDP-43 or cancer-related kinases making it significant in understanding these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

See full target information PFN1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70369 PubMed40202153

2025

Sevoflurane Inhibits the Proliferation of Neural Precursor Cells and Neural Migration of Mice by Inducing Iron Metabolism Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Xincheng Li,Runjiao Cheng,Mahammad Naeem,Xiaoou Nie,Jiaqi Wang,Liqiang Zhao,Xiaopeng Liu,Zhenhua Shi,Jianhua Zhang

Cells 11: PubMed36291059

2022

PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zi,Jing Xu,Jintang Luo,Xu Yang,Zhen Zhen,Xin Li,Debao Hu,Yiwen Guo,Hong Guo,Xiangbin Ding,Linlin Zhang

Journal of molecular biology 434:167397 PubMed34896361

2021

The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Drazic,Evy Timmerman,Ulrike Kajan,Michaël Marie,Sylvia Varland,Francis Impens,Kris Gevaert,Thomas Arnesen

Basic and clinical neuroscience 12:213-222 PubMed34925718

2021

Mutant Profilin1 Aggregation in Amyotrophic Lateral Sclerosis: An in Vivo Biochemical Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mina Nekouei,Atousa Aliahmadi,Mahmoud Kiaei,Ali Reza Ghassempour

Life science alliance 4: PubMed33184056

2020

The actin regulator profilin 1 is functionally associated with the mammalian centrosome.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela Nejedlá,Anastasiya Klebanovych,Vadym Sulimenko,Tetyana Sulimenko,Eduarda Dráberová,Pavel Dráber,Roger Karlsson

Science advances 6:eaay8793 PubMed32284999

2020

Mechanism of actin N-terminal acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Grzegorz Rebowski,Malgorzata Boczkowska,Adrian Drazic,Rasmus Ree,Marianne Goris,Thomas Arnesen,Roberto Dominguez

Development (Cambridge, England) 147: PubMed32098764

2020

Human antigen R-regulated mRNA metabolism promotes the cell motility of migrating mouse neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Fei Zhao,Xiao-Xiao He,Zi-Fei Song,Ye Guo,Yan-Ning Zhang,Hua-Li Yu,Zi-Xuan He,Wen-Cheng Xiong,Weixiang Guo,Xiao-Juan Zhu

The Journal of clinical investigation 130:2024-2040 PubMed31945017

2020

Profilin 1 delivery tunes cytoskeletal dynamics toward CNS axon regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Pinto-Costa,Sara C Sousa,Sérgio C Leite,Joana Nogueira-Rodrigues,Tiago Ferreira da Silva,Diana Machado,Joana Marques,Ana Catarina Costa,Márcia A Liz,Francesca Bartolini,Pedro Brites,Mercedes Costell,Reinhard Fässler,Mónica M Sousa

Cerebral cortex (New York, N.Y. : 1991) 30:3467-3482 PubMed31867667

2019

Profilin1-Dependent F-Actin Assembly Controls Division of Apical Radial Glia and Neocortex Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jan A Kullmann,Sophie Meyer,Fabrizia Pipicelli,Christina Kyrousi,Felix Schneider,Nora Bartels,Silvia Cappello,Marco B Rust

Current biology : CB 27:3535-3543.e4 PubMed29129529

2017

Profilin Directly Promotes Microtubule Growth through Residues Mutated in Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica L Henty-Ridilla,M Angeles Juanes,Bruce L Goode

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Profilin 1 antibody - Carboxyterminal end

Immunoprecipitation - Anti-Profilin 1 antibody - Carboxyterminal end (AB50667)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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