Knockout Tested Rabbit Recombinant Monoclonal Profilin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples.
Images
![Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (AB248536), expandable thumbnail](https://content.abcam.com/products/images/profilin-1-antibody-epr6303-bsa-and-azide-free-ab248536--western-blot-img2849.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (AB248536)")
![Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (AB248536), expandable thumbnail](https://content.abcam.com/products/images/profilin-1-antibody-epr6303-bsa-and-azide-free-ab248536--western-blot-img430390.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (AB248536)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Predicted | Expected |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Alternative names
Profilin-1, Epididymis tissue protein Li 184a, Profilin I, PFN1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal Profilin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6303
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248536 is the carrier-free version of Anti-Profilin 1 antibody [EPR6303] ab133529.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Profilin 1 also known as Profilin-1 or Profilin is a small actin-binding protein with a molecular weight of approximately 15 kDa. It is expressed across various tissues including muscle brain and epithelial cells. Profilin 1 interacts with monomeric actin (G-actin) to regulate actin polymerization by binding to actin monomers. This protein also interacts with polyphosphoinositides and proline-rich sequences playing a mechanical role in balancing actin filament assembly and disassembly.
- Biological function summary
Profilin 1 acts as an important regulator of the cytoskeleton facilitating cellular processes like motility and division. It participates in the formation of actin filaments by delivering actin monomers to barbed ends promoting polymerization. Profilin 1 is part of the complex that controls actin dynamics and this ability to modulate actin structure allows it to influence cell shape and internal movement. The protein's interactions help support the actin cytoskeleton for intracellular trafficking and signal transduction.
- Pathways
Profilin 1 has important roles in the regulation of the actin cytoskeleton and Rho signaling pathways. These pathways are important in cell migration and adhesion. During cellular processes Profilin 1's interactions with actin and polyphosphoinositides link it to other proteins such as Rho GTPases which are associated with cytoskeletal dynamics. Profilin 1 can influence these pathways by modulating actin dynamics and interacting with proteins involved in these signaling cascades.
- Associated diseases and disorders
Disruptions in Profilin 1 function are linked to neurodegenerative diseases and cancer metastasis. For example aberrant Profilin 1 activity can contribute to amyotrophic lateral sclerosis (ALS) by affecting neuronal structure and transport. Dysregulation of its interaction with actin or polyphosphoinositides might also lead to enhanced cancer cell migration and invasion. It may interact with other proteins implicated in these diseases such as the ALS-related protein TDP-43 or cancer-related kinases making it significant in understanding these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (ab248536), expandable thumbnail](https://content.abcam.com/products/images/profilin-1-antibody-epr6303-bsa-and-azide-free-ab248536--western-blot-img2849.jpg "Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (ab248536)")
Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (ab248536)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Profilin 1 antibody [EPR6303] ab133529).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Profilin 1 antibody [EPR6303] ab133529 observed at 15 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Profilin 1 antibody [EPR6303] ab133529 was shown to specifically react with in wild-type HAP1 cells as signal was lost in PFN1 knockout cells. Wild-type and PFN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Anti-Profilin 1 antibody [EPR6303] ab133529 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Profilin 1 antibody [EPR6303] (Anti-Profilin 1 antibody [EPR6303] ab133529) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: PFN1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: Jurkat whole cell lysate at 20 µg
Predicted band size: 15 kDa
![Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (ab248536), expandable thumbnail](https://content.abcam.com/products/images/profilin-1-antibody-epr6303-bsa-and-azide-free-ab248536--western-blot-img430390.jpg "Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (ab248536)")
Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (ab248536)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Profilin 1 antibody [EPR6303] ab133529)
Anti-Profilin 1 antibody [EPR6303] ab133529 was shown to react with PFN1 in wild-type HAP1 cells in Western blot with loss of signal observed in a PFN1 knockout cell line. Wild-type HAP1 and PFN1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Profilin 1 antibody [EPR6303] ab133529 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.
This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-Profilin 1 antibody [EPR6303] (Anti-Profilin 1 antibody [EPR6303] ab133529) at 1/10000 dilution
Lane 1: Wild-type HAP1 lysate at 20 µg
Lane 2: PFN1 knock-out HAP1 lysate at 20 µg
Observed band size: 15 kDa
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