Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free

• WB

Lab

Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (AB248536)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133529).

Lanes 1 - 4 : Merged signal (red and green). Green - ab133529 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133529 was shown to specifically react with in wild-type HAP1 cells as signal was lost in PFN1 knockout cells. Wild-type and PFN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab133529 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Profilin 1 antibody [EPR6303] (<a href='/en-us/products/primary-antibodies/profilin-1-antibody-epr6303-ab133529'>ab133529</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PFN1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

Jurkat whole cell lysate at 20 µg

Predicted band size: 15 kDa

false

• WB

Lab

Western blot - Anti-Profilin 1 antibody [EPR6303] - BSA and Azide free (AB248536)

This data was developed using the same antibody clone in a different buffer formulation (ab133529)

ab133529 was shown to react with PFN1 in wild-type HAP1 cells in Western blot with loss of signal observed in a PFN1 knockout cell line. Wild-type HAP1 and PFN1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133529 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Profilin 1 antibody [EPR6303] (<a href='/en-us/products/primary-antibodies/profilin-1-antibody-epr6303-ab133529'>ab133529</a>) at 1/10000 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

PFN1 knock-out HAP1 lysate at 20 µg

Observed band size: 15 kDa

false