Rabbit Polyclonal Profilin 1 phospho S138 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PFN1 phospho S138.
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Publications
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- Cellular and molecular neurobiology :2020A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkanksha Baharani,Zelan Wei,William J Roesler,Darrell D MousseauPubMed 33108594
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PFN1 phospho S138. The exact immunogen used to generate this antibody is proprietary information. Database link P07737
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Alternative names
Profilin-1, Epididymis tissue protein Li 184a, Profilin I, PFN1
Recommended products
Rabbit Polyclonal Profilin 1 phospho S138 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PFN1 phospho S138.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PFN1 phospho S138. The exact immunogen used to generate this antibody is proprietary information. Database link P07737
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab215752 was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Profilin 1 also known as Profilin-1 or Profilin is a small actin-binding protein with a molecular weight of approximately 15 kDa. It is expressed across various tissues including muscle brain and epithelial cells. Profilin 1 interacts with monomeric actin (G-actin) to regulate actin polymerization by binding to actin monomers. This protein also interacts with polyphosphoinositides and proline-rich sequences playing a mechanical role in balancing actin filament assembly and disassembly.
- Biological function summary
Profilin 1 acts as an important regulator of the cytoskeleton facilitating cellular processes like motility and division. It participates in the formation of actin filaments by delivering actin monomers to barbed ends promoting polymerization. Profilin 1 is part of the complex that controls actin dynamics and this ability to modulate actin structure allows it to influence cell shape and internal movement. The protein's interactions help support the actin cytoskeleton for intracellular trafficking and signal transduction.
- Pathways
Profilin 1 has important roles in the regulation of the actin cytoskeleton and Rho signaling pathways. These pathways are important in cell migration and adhesion. During cellular processes Profilin 1's interactions with actin and polyphosphoinositides link it to other proteins such as Rho GTPases which are associated with cytoskeletal dynamics. Profilin 1 can influence these pathways by modulating actin dynamics and interacting with proteins involved in these signaling cascades.
- Associated diseases and disorders
Disruptions in Profilin 1 function are linked to neurodegenerative diseases and cancer metastasis. For example aberrant Profilin 1 activity can contribute to amyotrophic lateral sclerosis (ALS) by affecting neuronal structure and transport. Dysregulation of its interaction with actin or polyphosphoinositides might also lead to enhanced cancer cell migration and invasion. It may interact with other proteins implicated in these diseases such as the ALS-related protein TDP-43 or cancer-related kinases making it significant in understanding these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Profilin 1 (phospho S138) antibody (ab215752)
Lanes 1 - 3: anti-Profilin 1 (aa 132-140)
Lanes 4 - 6: Western blot - Anti-Profilin 1 (phospho S138) antibody (ab215752) at 1/1000 dilution
Lanes 1 and 4: Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min
Lanes 2 and 5: Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min with unphosphorylated Profilin 1
Lanes 3 and 6: Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min with Profilin 1 phospho S132 peptide
Predicted band size: 15 kDa
Observed band size: 15 kDa
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