Rabbit Polyclonal Profilin 2 antibody. C-terminal. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFN2 aa 100 to C-terminus.
Images
Publications
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e231702023ELAVL4 promotes the tumorigenesis of small cell lung cancer by stabilizing LncRNA LYPLAL1-DT and enhancing profilin 2 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuxin Li,Jianyi Lv,Xing Zhang,Qiuyu Zhang,Zhihui Li,Jing Lu,Xueyun Huo,Meng Guo,Xin Liu,Ran Gao,Jianan Gong,Changlong Li,Weiying Li,Tongmei Zhang,Jinghui Wang,Zhenwen Chen,Xiaoyan DuPubMed 37676718
- Frontiers in cardiovascular medicine 9:7817532022Profilin 2 and Endothelial Exosomal Profilin 2 Promote Angiogenesis and Myocardial Infarction Repair in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenkun Li,Xueyun Huo,Keyan Chen,Fenghua Yang,Weijiang Tan,Qi Zhang,Haixu Yu,Changlong Li,Deshan Zhou,Hao Chen,Baoquan Zhao,Yuan Wang,Zhenwen Chen,Xiaoyan DuPubMed 35479278
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PFN2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35080
Reactivity data
Select an application| IHC-P | ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/cells for 6 µg/cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Alternative names
Profilin-2, Profilin II, PFN2
Recommended products
Rabbit Polyclonal Profilin 2 antibody. C-terminal. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFN2 aa 100 to C-terminus.
Alternative names
Profilin-2, Profilin II, PFN2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PFN2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35080
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Profilin 2 also known as PFN2 is an actin-binding protein with an important role in the regulation of the actin cytoskeleton. With a molecular weight of around 14 kDa it is expressed in tissues such as the brain and muscle where it contributes to dynamic cellular processes such as cell movement and shape maintenance. PFN2 binds not only to actin but also to phosphatidylinositol 45-bisphosphate mediating the sequestration and availability of actin monomers essential for actin polymerization.
- Biological function summary
Profilin 2 regulates actin dynamics essential for cellular processes like membrane trafficking and signal transduction. It is not typically known to be part of an extensive protein complex but interacts with other proteins including members of the Arp2/3 complex to promote actin filament nucleation and branching. This regulation impacts processes like vesicle transport particularly in neuronal cells where Profilin 2 influences synaptic function and plasticity.
- Pathways
Actin dynamics controlled by Profilin 2 play significant roles in both the Rho GTPase and PI3K/AKT signaling pathways. In these pathways Profilin 2 interfaces with Rho-related proteins to modulate the actin cytoskeleton impacting cell adhesion and movement. Its interaction with PI3K elements contributes to cell growth and survival signals aligning Profilin 2's functions with cellular responses to environmental cues.
- Associated diseases and disorders
Abnormalities in Profilin 2 expression or function have been linked to neurological disorders such as schizophrenia and neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS). In these conditions Profilin 2’s interactions with cytoskeletal proteins such as cofilin and tau could disrupt normal neuronal functions and synaptic stability. Its deregulation resulting in misbalanced actin dynamics contributes to the pathology observed in affected neuronal circuits.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-Profilin 2 antibody - C-terminal (ab191054)
Immunocytochemistry analysis of SH-SY5Y cells labelling Profilin 2 with ab191054 at 5 μg/mL overnight at 4°C.. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent and the cells were blocked with 10% goat serum. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 2 antibody - C-terminal (ab191054)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining Profilin 2 with ab191054 at 1μg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot - Anti-Profilin 2 antibody - C-terminal (ab191054)
All lanes: Western blot - Anti-Profilin 2 antibody - C-terminal (ab191054) at 0.5 µg/mL
Lane 1: Human placenta tissue lysates
Lane 2: Rat brain tissue lysates
Lane 3: Mouse brain tissue lysates
SecondaryAll lanes: Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 2 antibody - C-terminal (ab191054)
Immunohistochemical analysis of paraffin-embedded human intestincal cancer tissue staining Profilin 2 with ab191054 at 1μg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Flow Cytometry (Intracellular) - Anti-Profilin 2 antibody - C-terminal (ab191054)
Flow Cytometry analysis of A431 cells labeling Profilin 2 with ab191054 (1μg/1x106 cells), DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody (blue). Isotype control antibody was rabbit IgG (1μg/1x106) (green). An unlabelled samples was used as a second control (red). The cells were blocked with 10% normal goat serum
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