AB174322

Anti-Profilin 2 antibody [EPR11030]

RabMAb
Recombinant
KO Validated
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Profilin 2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Profilin-2, Profilin II, PFN2

3 Images

Supplier Data

Western blot - Anti-Profilin 2 antibody [EPR11030] (AB174322)

All lanes:

Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

MCF7 lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

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Supplier Data

Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (AB174322)

Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, immunoprecipitated using ab174322 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)

Predicted band size: 15 kDa

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Lab

Western blot - Anti-Profilin 2 antibody [EPR11030] (AB174322)

False colour image of Western blot : Anti-Profilin 2 antibody [EPR11030] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab174322 was shown to bind specifically to Profilin 2. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in PFN2 knockout cell line ab265833 (knockout cell lysate ab258108). To generate this image, wild-type and PFN2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PFN2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PFN2 (Profilin 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pfn2-profilin-2-knockout-hela-cell-line-ab265833'>ab265833</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 15 kDa

Observed band size: 13 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11030

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Profilin 2 also known as PFN2 is an actin-binding protein with an important role in the regulation of the actin cytoskeleton. With a molecular weight of around 14 kDa it is expressed in tissues such as the brain and muscle where it contributes to dynamic cellular processes such as cell movement and shape maintenance. PFN2 binds not only to actin but also to phosphatidylinositol 45-bisphosphate mediating the sequestration and availability of actin monomers essential for actin polymerization.

Biological function summary

Profilin 2 regulates actin dynamics essential for cellular processes like membrane trafficking and signal transduction. It is not typically known to be part of an extensive protein complex but interacts with other proteins including members of the Arp2/3 complex to promote actin filament nucleation and branching. This regulation impacts processes like vesicle transport particularly in neuronal cells where Profilin 2 influences synaptic function and plasticity.

Pathways

Actin dynamics controlled by Profilin 2 play significant roles in both the Rho GTPase and PI3K/AKT signaling pathways. In these pathways Profilin 2 interfaces with Rho-related proteins to modulate the actin cytoskeleton impacting cell adhesion and movement. Its interaction with PI3K elements contributes to cell growth and survival signals aligning Profilin 2's functions with cellular responses to environmental cues.

Abnormalities in Profilin 2 expression or function have been linked to neurological disorders such as schizophrenia and neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS). In these conditions Profilin 2’s interactions with cytoskeletal proteins such as cofilin and tau could disrupt normal neuronal functions and synaptic stability. Its deregulation resulting in misbalanced actin dynamics contributes to the pathology observed in affected neuronal circuits.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

See full target information PFN2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:52 PubMed40249603

2025

Pax6 Directly Regulates the Raver2/sFlt-1 Expression in Corneal Epithelial Cells to Maintain the Cornea's Avascular Privilege.

Applications

Unspecified application

Species

Unspecified reactive species

Haiqi Xiao,Li Hu,Xiongshi Lin,Linchang Liu,Xing Dong,Liting Yang,Ying Liu,Zhichong Wang,Ying Tian,Shuangyong Wang

Frontiers in molecular neuroscience 15:880382 PubMed35592113

2022

Stress Elicits Contrasting Effects on Rac1-Cofilin Signaling in the Hippocampus and Amygdala.

Applications

Unspecified application

Species

Unspecified reactive species

Mihika Bose,Mohammad Sarfaraz Nawaz,Rakhi Pal,Sumantra Chattarji

The Journal of cell biology 219: PubMed31816056

2019

NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Huang,De-Juan Yuan,Shao Li,Xue-Song Liang,Yue Gao,Xiao-Yan Lan,Hua-Min Qin,Yu-Fang Ma,Guang-Yin Xu,Melitta Schachner,Vladimir Sytnyk,Johannes Boltze,Quan-Hong Ma,Shen Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Profilin 2 antibody [EPR11030]

Western blot - Anti-Profilin 2 antibody [EPR11030] (AB174322)

Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (AB174322)

Western blot - Anti-Profilin 2 antibody [EPR11030] (AB174322)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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