Rabbit Recombinant Monoclonal Profilin 2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818), expandable thumbnail](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-bsa-and-azide-free-ab249818--western-blot-img165810.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818)")
![Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818), expandable thumbnail](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-bsa-and-azide-free-ab249818--immunoprecipitation-img74005.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818)")
![Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818), expandable thumbnail](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-bsa-and-azide-free-ab249818--western-blot-img64303.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Alternative names
Profilin-2, Profilin II, PFN2
Recommended products
Rabbit Recombinant Monoclonal Profilin 2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11030
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249818 is the carrier-free version of Anti-Profilin 2 antibody [EPR11030] ab174322.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Profilin 2 also known as PFN2 is an actin-binding protein with an important role in the regulation of the actin cytoskeleton. With a molecular weight of around 14 kDa it is expressed in tissues such as the brain and muscle where it contributes to dynamic cellular processes such as cell movement and shape maintenance. PFN2 binds not only to actin but also to phosphatidylinositol 45-bisphosphate mediating the sequestration and availability of actin monomers essential for actin polymerization.
- Biological function summary
Profilin 2 regulates actin dynamics essential for cellular processes like membrane trafficking and signal transduction. It is not typically known to be part of an extensive protein complex but interacts with other proteins including members of the Arp2/3 complex to promote actin filament nucleation and branching. This regulation impacts processes like vesicle transport particularly in neuronal cells where Profilin 2 influences synaptic function and plasticity.
- Pathways
Actin dynamics controlled by Profilin 2 play significant roles in both the Rho GTPase and PI3K/AKT signaling pathways. In these pathways Profilin 2 interfaces with Rho-related proteins to modulate the actin cytoskeleton impacting cell adhesion and movement. Its interaction with PI3K elements contributes to cell growth and survival signals aligning Profilin 2's functions with cellular responses to environmental cues.
- Associated diseases and disorders
Abnormalities in Profilin 2 expression or function have been linked to neurological disorders such as schizophrenia and neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS). In these conditions Profilin 2’s interactions with cytoskeletal proteins such as cofilin and tau could disrupt normal neuronal functions and synaptic stability. Its deregulation resulting in misbalanced actin dynamics contributes to the pathology observed in affected neuronal circuits.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818), expandable thumbnail](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-bsa-and-azide-free-ab249818--western-blot-img165810.jpg "Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)")
Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)
This data was developed using Anti-Profilin 2 antibody [EPR11030] ab174322, the same antibody clone in a different buffer formulation.
False colour image of Western blot: Anti-Profilin 2 antibody [EPR11030] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Profilin 2 antibody [EPR11030] ab174322 was shown to bind specifically to Profilin 2. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in PFN2 knockout cell line Human PFN2 (Profilin 2) knockout HeLa cell line ab265833 (knockout cell lysate Human PFN2 (Profilin 2) knockout HeLa cell lysate ab258108). To generate this image, wild-type and PFN2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Profilin 2 antibody [EPR11030] (Anti-Profilin 2 antibody [EPR11030] ab174322) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PFN2 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human PFN2 (Profilin 2) knockout HeLa cell line (Human PFN2 (Profilin 2) knockout HeLa cell line ab265833)
Lane 3: SH-SY5Y cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 13 kDa
![Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818), expandable thumbnail](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-bsa-and-azide-free-ab249818--immunoprecipitation-img74005.jpg "Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)")
Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)
This data was developed using Anti-Profilin 2 antibody [EPR11030] ab174322, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, immunoprecipitated using Anti-Profilin 2 antibody [EPR11030] ab174322 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (Anti-Profilin 2 antibody [EPR11030] ab174322)
Predicted band size: 15 kDa
![Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818), expandable thumbnail](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-bsa-and-azide-free-ab249818--western-blot-img64303.jpg "Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)")
Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)
This data was developed using Anti-Profilin 2 antibody [EPR11030] ab174322, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Profilin 2 antibody [EPR11030] (Anti-Profilin 2 antibody [EPR11030] ab174322) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: MCF7 lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa
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