Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free

• WB

Lab

Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818)

This data was developed using ab174322, the same antibody clone in a different buffer formulation.

False colour image of Western blot : Anti-Profilin 2 antibody [EPR11030] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab174322 was shown to bind specifically to Profilin 2. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in PFN2 knockout cell line ab265833 (knockout cell lysate ab258108). To generate this image, wild-type and PFN2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Profilin 2 antibody [EPR11030] (<a href='/en-us/products/primary-antibodies/profilin-2-antibody-epr11030-ab174322'>ab174322</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PFN2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PFN2 (Profilin 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pfn2-profilin-2-knockout-hela-cell-line-ab265833'>ab265833</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 15 kDa

Observed band size: 13 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818)

This data was developed using ab174322, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, immunoprecipitated using ab174322 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (<a href='/en-us/products/primary-antibodies/profilin-2-antibody-epr11030-ab174322'>ab174322</a>)

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (AB249818)

This data was developed using ab174322, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Profilin 2 antibody [EPR11030] (<a href='/en-us/products/primary-antibodies/profilin-2-antibody-epr11030-ab174322'>ab174322</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

MCF7 lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

false