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Rabbit Recombinant Monoclonal Lamin-A/C antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Progerin antibody [EPR28694-72] (AB320642), expandable thumbnail
  • Western blot - Anti-Progerin antibody [EPR28694-72] (AB320642), expandable thumbnail
  • Western blot - Anti-Progerin antibody [EPR28694-72] (AB320642), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progerin antibody [EPR28694-72] (AB320642), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progerin antibody [EPR28694-72] (AB320642), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIP
Human
Predicted
Predicted
Predicted
Predicted
Transfected cell line - Human
Not recommended
Tested
Tested
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/200
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Target data

Function

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:2188730, PubMed:22431096, PubMed:2344612, PubMed:23666920, PubMed:24741066, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:24741066, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:23990565, PubMed:25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2188730, PubMed:2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920). Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Alternative names

LMN1, Prelamin-A/C

Recommended products

Rabbit Recombinant Monoclonal Lamin-A/C antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28694-72
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Progerin also known as a truncated version of Lamin A (LMNA) is a mutant protein that emerges due to a cryptic splice site in the LMNA gene. This results in a 607 amino acid protein with a mass of approximately 65 kDa. Progerin is expressed in the cell nucleus and accumulates over time predominantly affecting cells with high turnover rates such as those in the skin cardiovascular system and adipose tissues. This abnormal protein disrupts the normal function of the nuclear lamina.

Biological function summary

Progerin acts by compromising cellular structural integrity and altering gene expression. It integrates into the nuclear lamina but fails to undergo proper post-translational modification which is vital for cellular functions like mitosis nuclear migration and chromatin organization. Progerin's presence affects the mechanical properties of the nucleus hindering its role in stress response and inducing cellular aging. This mutant protein functions independently as it does not form part of a larger protein complex but rather disturbs the assembly of the lamina network.

Pathways

Progerin influences the mechanistic pathways responsible for cellular aging and apoptosis. It disrupts the Wnt/β-catenin pathway affecting cell cycle regulation and the MAPK signaling pathway which relates to stress and growth responses. Progerin shares a close association with prelamin A and Lamin C as all these proteins derive from the same LMNA gene and interact through shared pathways influencing nuclear shape and transcriptome stability.

Associated diseases and disorders

Progerin plays a significant role in Hutchinson-Gilford Progeria Syndrome (HGPS) and is implicated in age-related disorders like arteriosclerosis. HGPS results from the accumulation of Progerin leading to premature aging due to the faulty nuclear architecture. Progerin's relation with other proteins such as Lamin B1 and Lamin C affects the nuclear envelope's integrity exacerbating the progression of these disorders. The presence of Progerin may serve as a potential target for therapeutic interventions aimed at blocking its production or function which could mitigate its effects on aging-related pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Progerin antibody [EPR28694-72] (ab320642), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Progerin antibody [EPR28694-72] (ab320642)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Progerin with ab320642 at 1/200 (2.545 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing nuclear envelope staining in 293T cells transfected with human Progerin expression vector containing a Flag tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-FLAG mouse monoclonal antibody was used to counterstain tubulin at 1/500 2ug/ml dilution (Magenta), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed antibody at 1/1000 2 ug/mL dilution. The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Western blot - Anti-Progerin antibody [EPR28694-72] (ab320642), expandable thumbnail

    Western blot - Anti-Progerin antibody [EPR28694-72] (ab320642)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Western blot analysis of HEK-293T cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate 2 ug mixed with HeLa whole cell lysate 20 ug. The membrane was cut into strips and each strip was incubated separately with the following antibodies: Lane 1: anti Lamin A + Lamin C antibody Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker ab108595 (1:1000), Lane 2: anti Flag-tag antibody (1:10000), Lane 3: anti-progerin ab320642 (1:1000).

    The anti-progerin antibody ab320642 specifically detects human progerin.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12714972, PMID: 33408413, PMID: 34694158).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Lamin A + Lamin C antibody - (Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker ab108595) staining at 64-76KDa dilution.

    In Western blot, Anti-Flag tag staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Progerin antibody [EPR28694-72] (ab320642) at 1/1000 dilution

    All lanes: HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate 2 μg mixed with HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 74 kDa, 64-76 kDa

    Exposure time: 81s

  • Western blot - Anti-Progerin antibody [EPR28694-72] (ab320642), expandable thumbnail

    Western blot - Anti-Progerin antibody [EPR28694-72] (ab320642)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human Lamin A and Lamin C.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Lamin A + Lamin C antibody - (Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker ab108595) staining at 64-76KDa dilution.

    In Western blot, Anti-Flag tag staining at 1/10000 dilution.

    In Western blot, (Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Progerin antibody [EPR28694-72] (ab320642) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a DDDDK-tag, whole cell lysate at 20 µg

    Lane 2: HEK-293T cells transfected with a human progerin expression vector containing a Flag-tag, whole cell lysate at 20 µg

    Lane 3: HEK-293T cells transfected with a human Lamin C expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: HEK-293T cells transfected with a human Lamin A expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 74 kDa, 36 kDa, 64-76 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progerin antibody [EPR28694-72] (ab320642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progerin antibody [EPR28694-72] (ab320642)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human progerin expression vector containing a Flag-tag and (B) HEK-293T transfected with an empty vector containing a Flag-tag. tissue labeling Progerin with ab320642 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (A) HEK-293T transfected with a human progerin expression vector containing a Flag-tag. No staining in (B) HEK-293T transfected with an empty vector containing a Flag-tag.

    The section was incubated with ab320642 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progerin antibody [EPR28694-72] (ab320642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progerin antibody [EPR28694-72] (ab320642)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Progerin with ab320642 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human liver (PMID: 36497176).

    The section was incubated with ab320642 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunoprecipitation - Anti-Progerin antibody [EPR28694-72] (ab320642), expandable thumbnail

    Immunoprecipitation - Anti-Progerin antibody [EPR28694-72] (ab320642)

    Progerin was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate with ab320642 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab320642 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human progerin expression vector containing a Flag-tag, whole cell lysate

    Lane 2: ab320642 IP in HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab320642 in HEK-293T cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-Progerin antibody [EPR28694-72] (ab320642) at 1/30 dilution

    All lanes: HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 6s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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