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AB215527

Anti-Progesterone antibody [EPPTX-R1]

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(1 Publication)

Rabbit Recombinant Monoclonal Progesterone antibody. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

Pregn 4 ene 3 20 dione

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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] (AB215527)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] (AB215527)

ab215527 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis Inc.

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] (AB215527)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] (AB215527)

Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0 10 25 50 100 300 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis Inc.

  • Carrier free

    Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free

  • Carrier free

    Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R1

Isotype

IgG

Carrier free

No

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p>Use 3-5 ng/ml.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Progesterone also called P4 is a steroid hormone playing an important role in the reproductive system. Its molecular weight is approximately 314.47 Da. This hormone is mainly expressed in the ovaries but also in the adrenal glands and placenta. Progesterone acts by binding to its receptor the progesterone receptor (PR) which is found in target tissues such as the uterus mammary glands and the brain. When progesterone binds to PR it translocates to the nucleus to act as a transcription factor regulating gene expression that is important for reproductive function.
Biological function summary

Progesterone is essential for preparation and maintenance of pregnancy. It facilitates the transformation of the endometrium to a secretory phase to support embryo implantation. It also modulates immune responses during pregnancy to prevent the maternal immune system from rejecting the fetus. Progesterone and its receptor are not part of a larger protein complex but they do interact with other molecular pathways to exert their actions. Additionally progesterone inhibits the proliferation of mammary gland epithelial cells contributing to breast development and function.

Pathways

Progesterone plays a significant role in the menstrual cycle and pregnancy-related pathways. It works within the ovarian steroidogenesis pathway which includes other hormones like estrogen and luteinizing hormone. This pathway ensures the release and regulation of female sex hormones. Progesterone also interacts with the MAPK signaling pathway which involves proteins such as ERK1/2 affecting cellular processes including cell growth and differentiation.

Progesterone is linked to conditions such as endometriosis and breast cancer. In endometriosis progesterone resistance leads to the abnormal growth of endometrial tissue outside the uterus. The hormone's association with breast cancer involves its interaction with proteins like estrogen receptor alpha which can promote cancer cell proliferation when dysregulated. Understanding progesterone’s role in disease helps in developing therapeutic strategies to manage these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Progesterone
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 12:705012 PubMed34335537

2021

Light-Stress Response Mediated by the Transcription Factor Mga2 in the Yeast .

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Camponeschi,Arianna Montanari,Marzia Beccaccioli,Massimo Reverberi,Cristina Mazzoni,Michele M Bianchi
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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