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AB222404

Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Progesterone antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.
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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free (AB222404)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free (AB222404)

ab222404 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis Inc.

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free (AB222404)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free (AB222404)

This ELISA data used the same anti-Progesterone antibody clone [EPPTX-R1] in a different buffer formulation (cat# ab215527).

Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0 10 25 50 100 300 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis Inc.

  • Unconjugated

    Anti-Progesterone antibody [EPPTX-R1]

  • Carrier free

    Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R1

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB20236

Anti-Progesterone antibody [11P14]

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Complementary Products

Assay Kits

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AB271997

Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free

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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (AB271997)

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Primary Antibodies

AB20236

Anti-Progesterone antibody [11P14]

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Primary Antibodies

AB215527

Anti-Progesterone antibody [EPPTX-R1]

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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] (AB215527)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab222404 is the carrier-free version of ab215527.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Progesterone also called P4 is a steroid hormone playing an important role in the reproductive system. Its molecular weight is approximately 314.47 Da. This hormone is mainly expressed in the ovaries but also in the adrenal glands and placenta. Progesterone acts by binding to its receptor the progesterone receptor (PR) which is found in target tissues such as the uterus mammary glands and the brain. When progesterone binds to PR it translocates to the nucleus to act as a transcription factor regulating gene expression that is important for reproductive function.
Biological function summary

Progesterone is essential for preparation and maintenance of pregnancy. It facilitates the transformation of the endometrium to a secretory phase to support embryo implantation. It also modulates immune responses during pregnancy to prevent the maternal immune system from rejecting the fetus. Progesterone and its receptor are not part of a larger protein complex but they do interact with other molecular pathways to exert their actions. Additionally progesterone inhibits the proliferation of mammary gland epithelial cells contributing to breast development and function.

Pathways

Progesterone plays a significant role in the menstrual cycle and pregnancy-related pathways. It works within the ovarian steroidogenesis pathway which includes other hormones like estrogen and luteinizing hormone. This pathway ensures the release and regulation of female sex hormones. Progesterone also interacts with the MAPK signaling pathway which involves proteins such as ERK1/2 affecting cellular processes including cell growth and differentiation.

Progesterone is linked to conditions such as endometriosis and breast cancer. In endometriosis progesterone resistance leads to the abnormal growth of endometrial tissue outside the uterus. The hormone's association with breast cancer involves its interaction with proteins like estrogen receptor alpha which can promote cancer cell proliferation when dysregulated. Understanding progesterone’s role in disease helps in developing therapeutic strategies to manage these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Progesterone
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:2595-2609 PubMed32028262

2020

Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hu,Jianghong Yu,Jun Gan,Ning Song,Liubin Shi,Jie Liu,Ziqiang Zhang,Jianjun Du
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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