AB131486

Anti-Progesterone Receptor antibody

2

(2 Reviews)

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(14 Publications)

Rabbit Polyclonal Progesterone Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human PGR aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NR3C3, PGR, Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (AB131486)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (AB131486)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling Progesterone Receptor with ab131486 at 1/100 dilution.

Western blot - Anti-Progesterone Receptor antibody (AB131486)

WB

Unknown

Western blot - Anti-Progesterone Receptor antibody (AB131486)

All lanes:

Western blot - Anti-Progesterone Receptor antibody (ab131486) at 1/500 dilution

All lanes:

MDA435 cell extract

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

Immunogen

Synthetic Peptide within Human PGR aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (AB75857)

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Complementary Products

Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131486 was purified by affinity chromatography using epitope specific peptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The progesterone receptor (PR) also known as NR3C3 is a nuclear receptor that functions as a transcription factor in response to the hormone progesterone. This receptor has a mass of approximately 99 kDa and is expressed in tissues such as the reproductive organs including the uterus ovaries and mammary glands. It is also found in non-reproductive tissues like the brain and bone. The receptor has two main isoforms PR-A and PR-B which differ slightly in structure but have unique biological roles.

Biological function summary

The progesterone receptor plays a significant role in regulating gene expression related to reproductive processes. PR is not part of a larger complex by itself but interacts with various coactivators and corepressors to modulate transcription. In the uterus and mammary glands PR mediates the effects of progesterone by promoting cell proliferation and preparing tissues for pregnancy. In other systems PR also links to various metabolic and immunological pathways influencing cell cycle progression and immune response.

Pathways

Progesterone receptor activity is integrated within the reproductive hormone signaling pathways and the Wnt signaling pathway. The receptor interacts directly with key proteins such as estrogen receptor (ER) and steroid receptor coactivator (SRC) complexes which are pivotal in modulating response to hormonal signals. These interactions underline the essential role of PR in maintaining hormonal balance and regulating reproductive functions.

The progesterone receptor associates with breast cancer and endometriosis. Aberrant expression or mutations in PR can contribute to the development and progression of breast cancer often linked with the estrogen receptor's influence. In endometriosis PR's altered functionality affects cellular response to progesterone contributing to tissue growth outside the uterus. These conditions also involve interactions with proteins like BRCA1 in breast cancer highlighting how PR connects to broader cellular and pathological networks.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as a transcriptional activator or repressor.. Isoform A. Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.. Isoform B. Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.. Isoform 4. Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

See full target information PGR

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5665 PubMed37704631

2023

MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zinab O Doha,Xiaoyan Wang,Nicholas L Calistri,Jennifer Eng,Colin J Daniel,Luke Ternes,Eun Na Kim,Carl Pelz,Michael Munks,Courtney Betts,Sunjong Kwon,Elmar Bucher,Xi Li,Trent Waugh,Zuzana Tatarova,Dylan Blumberg,Aaron Ko,Nell Kirchberger,Jennifer A Pietenpol,Melinda E Sanders,Ellen M Langer,Mu-Shui Dai,Gordon Mills,Koei Chin,Young Hwan Chang,Lisa M Coussens,Joe W Gray,Laura M Heiser,Rosalie C Sears

Endocrinology 162: PubMed33724348

2021

Exposure to Propylparaben During Pregnancy and Lactation Induces Long-Term Alterations to the Mammary Gland in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua P Mogus,Charlotte D LaPlante,Ruby Bansal,Klara Matouskova,Benjamin R Schneider,Elizabeth Daniele,Shannon J Silva,Mary J Hagen,Karen A Dunphy,D Joseph Jerry,Sallie S Schneider,Laura N Vandenberg

Communications biology 2:406 PubMed31701034

2019

Single-cell RNA-sequencing analysis of estrogen- and endocrine-disrupting chemical-induced reorganization of mouse mammary gland.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Kanaya,Gregory Chang,Xiwei Wu,Kohei Saeki,Lauren Bernal,Hyun-Jeong Shim,Jinhui Wang,Charles Warden,Takuro Yamamoto,Jay Li,June-Soo Park,Timothy Synold,Steve Vonderfecht,Michele Rakoff,Susan L Neuhausen,Shiuan Chen

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 15:62-79 PubMed31606604

2019

Inhibiting Pathways Predicted From a Steroid Hormone Gene Signature Yields Synergistic Antitumor Effects in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulaziz A Almotlak,Mariya Farooqui,Jill M Siegfried

Reproductive toxicology (Elmsford, N.Y.) 92:66-77 PubMed31408669

2019

Exposure to low doses of oxybenzone during perinatal development alters mammary gland morphology in male and female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Klara Matouskova,D Joseph Jerry,Laura N Vandenberg

Breast cancer research : BCR 21:85 PubMed31366361

2019

Claudin-low-like mouse mammary tumors show distinct transcriptomic patterns uncoupled from genomic drivers.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Fougner,Helga Bergholtz,Raoul Kuiper,Jens Henrik Norum,Therese Sørlie

International journal of cancer 146:1125-1138 PubMed31219615

2019

GLI1-induced mammary gland tumours are transplantable and maintain major molecular features.

Applications

Unspecified application

Species

Unspecified reactive species

Jens Henrik Norum,Oliver Frings,Maria Kasper,Helga Bergholtz,Helene Zell Thime,Åsa Bergström,Agneta Andersson,Raoul Kuiper,Erik Fredlund,Therese Sørlie,Rune Toftgård

International journal of biological sciences 15:522-532 PubMed30745839

2019

Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfei Ji,Yaqin Shi,Xin Wang,Weiwei He,Lin Tang,Shengwang Tian,Hua Jiang,Yongqian Shu,Xiaoxiang Guan

Journal of the Endocrine Society 2:903-921 PubMed30057971

2018

Oxybenzone Alters Mammary Gland Morphology in Mice Exposed During Pregnancy and Lactation.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte D LaPlante,Ruby Bansal,Karen A Dunphy,D Joseph Jerry,Laura N Vandenberg

Applied immunohistochemistry & molecular morphology : AIMM 27:491-500 PubMed29629944

2018

Human Endometriosis Tissue Microarray Reveals Site-specific Expression of Estrogen Receptors, Progesterone Receptor, and Ki67.

Applications

Unspecified application

Species

Unspecified reactive species

Mariano Colón-Caraballo,Miosotis García,Adalberto Mendoza,Idhaliz Flores

View all publications

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