AB268223

Anti-Progesterone Receptor antibody [PGR/2694] - BSA and Azide free

Name: Anti-Progesterone Receptor antibody [PGR/2694] - BSA and Azide free (ab268223) | Abcam
Brand: Abcam Limited
SKU: AB268223
Price: 905 USD
Availability: InStock

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Mouse Monoclonal Progesterone Receptor antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PGR aa 450-600.

View Alternative Names

NR3C3, PGR, Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [PGR/2694] - BSA and Azide free (AB268223)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [PGR/2694] - BSA and Azide free (AB268223)

Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for Progesterone Receptor using ab268102 at 2 μg/ml in immunohistochemical analysis.

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268102).

Protein Array - Anti-Progesterone Receptor antibody [PGR/2694] - BSA and Azide free (AB268223)

Protein Array

Unknown

Protein Array - Anti-Progesterone Receptor antibody [PGR/2694] - BSA and Azide free (AB268223)

Protein Array containing more than 19,000 full-length human proteins using ab268102.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268102).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PGR/2694

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PGR aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

P06401

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor concentrate

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The progesterone receptor (PR) also known as NR3C3 is a nuclear receptor that functions as a transcription factor in response to the hormone progesterone. This receptor has a mass of approximately 99 kDa and is expressed in tissues such as the reproductive organs including the uterus ovaries and mammary glands. It is also found in non-reproductive tissues like the brain and bone. The receptor has two main isoforms PR-A and PR-B which differ slightly in structure but have unique biological roles.

Biological function summary

The progesterone receptor plays a significant role in regulating gene expression related to reproductive processes. PR is not part of a larger complex by itself but interacts with various coactivators and corepressors to modulate transcription. In the uterus and mammary glands PR mediates the effects of progesterone by promoting cell proliferation and preparing tissues for pregnancy. In other systems PR also links to various metabolic and immunological pathways influencing cell cycle progression and immune response.

Pathways

Progesterone receptor activity is integrated within the reproductive hormone signaling pathways and the Wnt signaling pathway. The receptor interacts directly with key proteins such as estrogen receptor (ER) and steroid receptor coactivator (SRC) complexes which are pivotal in modulating response to hormonal signals. These interactions underline the essential role of PR in maintaining hormonal balance and regulating reproductive functions.

The progesterone receptor associates with breast cancer and endometriosis. Aberrant expression or mutations in PR can contribute to the development and progression of breast cancer often linked with the estrogen receptor's influence. In endometriosis PR's altered functionality affects cellular response to progesterone contributing to tissue growth outside the uterus. These conditions also involve interactions with proteins like BRCA1 in breast cancer highlighting how PR connects to broader cellular and pathological networks.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as a transcriptional activator or repressor.. Isoform A. Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.. Isoform B. Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.. Isoform 4. Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

See full target information PGR

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