Anti-Progesterone Receptor antibody [SP2]

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-positive breast carcinoma tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain. Panel A : merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-positive breast carcinoma. Panel B : anti-PR stained on nucleus of cancer cells. Panel C : anti-HER2 stained on membrane of cancer cells. Panel D : anti-ER stained on nucleus of cancer cells. The section was incubated in three rounds of staining : in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• Flow Cyt

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Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/220 dilution (1.04 μg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

This image was generated from the hybridoma version.

• Flow Cyt

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Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5000 events was performed.

This image was generated from the hybridoma version.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.

This image was generated from the hybridoma version.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/100 (2.28 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated from the hybridoma version.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mammary gland tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain. Panel A : merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human mammary gland. Panel B : anti-PR stained on nucleus of some ductal cells. Panel C : anti-HER2 stained on no cells. Panel D : anti-ER stained on nucleus of some ductal cells. The section was incubated in three rounds of staining : in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-negative breast carcinoma tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain. Panel A : merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-negative breast carcinoma. Panel B : anti-PR stained on no cells. Panel C : anti-HER2 stained on no cells. Panel D : anti-ER stained on no cells. The section was incubated in three rounds of staining : in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (AB16661)

Rat mammary epithelial cells stained for Pr (pink) using ab16661 at 1/100 dilution in ICC/IF.

Ding L, et al. (2019), PLOS Genetics, 15(3), e1008002. Fig 3D, DOI: 10.1371/journal.pgen.1008002. Reproduced under the Creative Commons licence. https://creativecommons.org/licenses/by/4.0/