Rabbit Monoclonal Progesterone Receptor antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Mouse, Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | Flow Cyt | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected |
Rat | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as a transcriptional activator or repressor.Isoform ALigand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.Isoform BTranscriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.Isoform 4Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
NR3C3, NR3C3, PGR, Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3
Rabbit Monoclonal Progesterone Receptor antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Mouse, Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
SP42
Affinity purification Protein A/G
Purified from TCS by protein A/G.
Blue Ice
+4°C
+4°C
Do Not Freeze
ab236222 is the carrier-free version of Anti-Progesterone Receptor antibody [SP42] ab101688.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
The progesterone receptor (PR) also known as NR3C3 is a nuclear receptor that functions as a transcription factor in response to the hormone progesterone. This receptor has a mass of approximately 99 kDa and is expressed in tissues such as the reproductive organs including the uterus ovaries and mammary glands. It is also found in non-reproductive tissues like the brain and bone. The receptor has two main isoforms PR-A and PR-B which differ slightly in structure but have unique biological roles.
The progesterone receptor plays a significant role in regulating gene expression related to reproductive processes. PR is not part of a larger complex by itself but interacts with various coactivators and corepressors to modulate transcription. In the uterus and mammary glands PR mediates the effects of progesterone by promoting cell proliferation and preparing tissues for pregnancy. In other systems PR also links to various metabolic and immunological pathways influencing cell cycle progression and immune response.
Progesterone receptor activity is integrated within the reproductive hormone signaling pathways and the Wnt signaling pathway. The receptor interacts directly with key proteins such as estrogen receptor (ER) and steroid receptor coactivator (SRC) complexes which are pivotal in modulating response to hormonal signals. These interactions underline the essential role of PR in maintaining hormonal balance and regulating reproductive functions.
The progesterone receptor associates with breast cancer and endometriosis. Aberrant expression or mutations in PR can contribute to the development and progression of breast cancer often linked with the estrogen receptor's influence. In endometriosis PR's altered functionality affects cellular response to progesterone contributing to tissue growth outside the uterus. These conditions also involve interactions with proteins like BRCA1 in breast cancer highlighting how PR connects to broader cellular and pathological networks.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified Anti-Progesterone Receptor antibody [SP42] ab101688 at 1:260 dilution (1.02 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236222)
Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified Anti-Progesterone Receptor antibody [SP42] ab101688 at 1:100 (2.66 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236222)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution (0.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-Progesterone Receptor antibody [SP42] ab101688 for 30 mins at room temperature.
This image was generated using Anti-Progesterone Receptor antibody [SP42] ab101688, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution (0.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-Progesterone Receptor antibody [SP42] ab101688 for 30 mins at room temperature.
This image was generated using Anti-Progesterone Receptor antibody [SP42] ab101688, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution (0.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-Progesterone Receptor antibody [SP42] ab101688 for 30 mins at room temperature.
This image was generated using Anti-Progesterone Receptor antibody [SP42] ab101688, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedde sections) analysis of Human breast tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution (0.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-Progesterone Receptor antibody [SP42] ab101688 for 30 mins at room temperature.
This image was generated using Anti-Progesterone Receptor antibody [SP42] ab101688, the same clone, but with a different buffer formulation.
Anti-Progesterone Receptor antibody [SP42] ab101688, at a 1/400 dilution, staining Progesterone Receptor in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Progesterone Receptor antibody [SP42] ab101688).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast tissue, performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Progesterone Receptor antibody [SP42] ab101688).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer tissue, performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Progesterone Receptor antibody [SP42] ab101688).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast tissue, performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Progesterone Receptor antibody [SP42] ab101688).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with Anti-Progesterone Receptor antibody [SP42] ab101688 at 1/400 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast tissue, performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Progesterone Receptor antibody [SP42] ab101688).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com