Rabbit Polyclonal Progesterone Receptor phospho S190 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PGR phospho S190.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PGR phospho S190. The exact immunogen used to generate this antibody is proprietary information. Database link P06401
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as a transcriptional activator or repressor. Isoform A. Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2. Isoform B. Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation. Isoform 4. Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
Alternative names
NR3C3, PGR, Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3
Recommended products
Rabbit Polyclonal Progesterone Receptor phospho S190 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PGR phospho S190.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PGR phospho S190. The exact immunogen used to generate this antibody is proprietary information. Database link P06401
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab131110 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The progesterone receptor (PR) also known as NR3C3 is a nuclear receptor that functions as a transcription factor in response to the hormone progesterone. This receptor has a mass of approximately 99 kDa and is expressed in tissues such as the reproductive organs including the uterus ovaries and mammary glands. It is also found in non-reproductive tissues like the brain and bone. The receptor has two main isoforms PR-A and PR-B which differ slightly in structure but have unique biological roles.
- Biological function summary
The progesterone receptor plays a significant role in regulating gene expression related to reproductive processes. PR is not part of a larger complex by itself but interacts with various coactivators and corepressors to modulate transcription. In the uterus and mammary glands PR mediates the effects of progesterone by promoting cell proliferation and preparing tissues for pregnancy. In other systems PR also links to various metabolic and immunological pathways influencing cell cycle progression and immune response.
- Pathways
Progesterone receptor activity is integrated within the reproductive hormone signaling pathways and the Wnt signaling pathway. The receptor interacts directly with key proteins such as estrogen receptor (ER) and steroid receptor coactivator (SRC) complexes which are pivotal in modulating response to hormonal signals. These interactions underline the essential role of PR in maintaining hormonal balance and regulating reproductive functions.
- Associated diseases and disorders
The progesterone receptor associates with breast cancer and endometriosis. Aberrant expression or mutations in PR can contribute to the development and progression of breast cancer often linked with the estrogen receptor's influence. In endometriosis PR's altered functionality affects cellular response to progesterone contributing to tissue growth outside the uterus. These conditions also involve interactions with proteins like BRCA1 in breast cancer highlighting how PR connects to broader cellular and pathological networks.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody (ab131110)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling Progesterone Receptor (phospho S190) with ab131110 at 1/50 dilution.
Western blot - Anti-Progesterone Receptor (phospho S190) antibody (ab131110)
Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking.
All lanes: Western blot - Anti-Progesterone Receptor (phospho S190) antibody (ab131110) at 1/500 dilution
Lane 1: SKOV3 cell extracts, untreated
Lane 2: SKOV3 cell extracts, treated with EGF
Predicted band size: 98 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor (phospho S190) antibody (ab131110)
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF cells labelling Progesterone Receptor (phospho S190) with ab131110 at 1/100 dilution.
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