Rabbit Recombinant Monoclonal Progesterone Receptor phospho S190 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (AB75857), expandable thumbnail](https://content.abcam.com/products/images/progesterone-receptor-phospho-s190-antibody-ep1516y-ab75857--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140150.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (AB75857)")
Publications
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- Antioxidants (Basel, Switzerland) 11:2022Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuzan Attia Mawed,Carlotta Marini,Mahmoud Alagawany,Mayada R Farag,Rasha M Reda,Mohamed T El-Saadony,Walaa M Elhady,Gian E Magi,Alessandro Di Cerbo,Wafaa G El-NagarPubMed 36009286
- Cell stem cell 23:742-757.e82018SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJack-Christophe Cossec et. al.PubMed 30401455
- The American journal of pathology 176:1639-472010Multiplexed assessment of the Southwest Oncology Group-directed Intergroup Breast Cancer Trial S9313 by AQUA shows that both high and low levels of HER2 are associated with poor outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalini Harigopal et. al.PubMed 20150438
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.Isoform ALigand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.Isoform BTranscriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.Isoform 4Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
Alternative names
Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, NR3C3, PGR
Recommended products
Rabbit Recombinant Monoclonal Progesterone Receptor phospho S190 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP1516Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The progesterone receptor (PR) also known as NR3C3 is a nuclear receptor that functions as a transcription factor in response to the hormone progesterone. This receptor has a mass of approximately 99 kDa and is expressed in tissues such as the reproductive organs including the uterus ovaries and mammary glands. It is also found in non-reproductive tissues like the brain and bone. The receptor has two main isoforms PR-A and PR-B which differ slightly in structure but have unique biological roles.
- Biological function summary
The progesterone receptor plays a significant role in regulating gene expression related to reproductive processes. PR is not part of a larger complex by itself but interacts with various coactivators and corepressors to modulate transcription. In the uterus and mammary glands PR mediates the effects of progesterone by promoting cell proliferation and preparing tissues for pregnancy. In other systems PR also links to various metabolic and immunological pathways influencing cell cycle progression and immune response.
- Pathways
Progesterone receptor activity is integrated within the reproductive hormone signaling pathways and the Wnt signaling pathway. The receptor interacts directly with key proteins such as estrogen receptor (ER) and steroid receptor coactivator (SRC) complexes which are pivotal in modulating response to hormonal signals. These interactions underline the essential role of PR in maintaining hormonal balance and regulating reproductive functions.
- Associated diseases and disorders
The progesterone receptor associates with breast cancer and endometriosis. Aberrant expression or mutations in PR can contribute to the development and progression of breast cancer often linked with the estrogen receptor's influence. In endometriosis PR's altered functionality affects cellular response to progesterone contributing to tissue growth outside the uterus. These conditions also involve interactions with proteins like BRCA1 in breast cancer highlighting how PR connects to broader cellular and pathological networks.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
ab75857 at 1/100 dilution staining Progesterone Receptor in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857), expandable thumbnail](https://content.abcam.com/products/images/progesterone-receptor-phospho-s190-antibody-ep1516y-ab75857--western-blot-img139735.gif "Western blot - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)")
Western blot - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
All lanes: Western blot - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857) at 1/10000 dilution
Lane 1: T47D cell lysates, untreated at 10 µg
Lane 2: T47D cell lysates, treated with progesterone at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: 118 kDa, 99 kDa
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