AB61785

Anti-Progesterone Receptor (phospho S294) antibody

Name: Anti-Progesterone Receptor (phospho S294) antibody (ab61785) | Abcam
Brand: Abcam Limited
SKU: AB61785
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Progesterone Receptor phospho S294 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PGR phospho S294 aa 250-350.

View Alternative Names

NR3C3, PGR, Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor (phospho S294) antibody (AB61785)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor (phospho S294) antibody (AB61785)

Immunofluorescence analysis of HeLa cells, using ab61784 antibody at 1/500 dilution in the presence and abscence of immunizing peptide.

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Western blot - Anti-Progesterone Receptor (phospho S294) antibody (AB61785)

All lanes:

Western blot - Anti-Progesterone Receptor (phospho S294) antibody (ab61785) at 1/500 dilution

Lane 1:

extracts from Jurkat cells, treated with Etoposide (25uM, 60mins) with no immunizing peptide

Lane 2:

extracts from Jurkat cells, treated with Etoposide (25uM, 60mins) with immnunizing peptide

Predicted band size: 98 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human PGR phospho S294 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P06401

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified from rabbit antiserum by affinity chromatography usingepitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The progesterone receptor (PR) also known as NR3C3 is a nuclear receptor that functions as a transcription factor in response to the hormone progesterone. This receptor has a mass of approximately 99 kDa and is expressed in tissues such as the reproductive organs including the uterus ovaries and mammary glands. It is also found in non-reproductive tissues like the brain and bone. The receptor has two main isoforms PR-A and PR-B which differ slightly in structure but have unique biological roles.

Biological function summary

The progesterone receptor plays a significant role in regulating gene expression related to reproductive processes. PR is not part of a larger complex by itself but interacts with various coactivators and corepressors to modulate transcription. In the uterus and mammary glands PR mediates the effects of progesterone by promoting cell proliferation and preparing tissues for pregnancy. In other systems PR also links to various metabolic and immunological pathways influencing cell cycle progression and immune response.

Pathways

Progesterone receptor activity is integrated within the reproductive hormone signaling pathways and the Wnt signaling pathway. The receptor interacts directly with key proteins such as estrogen receptor (ER) and steroid receptor coactivator (SRC) complexes which are pivotal in modulating response to hormonal signals. These interactions underline the essential role of PR in maintaining hormonal balance and regulating reproductive functions.

The progesterone receptor associates with breast cancer and endometriosis. Aberrant expression or mutations in PR can contribute to the development and progression of breast cancer often linked with the estrogen receptor's influence. In endometriosis PR's altered functionality affects cellular response to progesterone contributing to tissue growth outside the uterus. These conditions also involve interactions with proteins like BRCA1 in breast cancer highlighting how PR connects to broader cellular and pathological networks.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as a transcriptional activator or repressor.. Isoform A. Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2.. Isoform B. Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.. Isoform 4. Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

See full target information PGR phospho S294

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:1770-1798 PubMed38565950

2024

LATS1 controls CTCF chromatin occupancy and hormonal response of 3D-grown breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julieta Ramírez-Cuéllar,Roberto Ferrari,Rosario T Sanz,Marta Valverde-Santiago,Judith García-García,A Silvina Nacht,David Castillo,Francois Le Dily,Maria Victoria Neguembor,Marco Malatesta,Sarah Bonnin,Marc A Marti-Renom,Miguel Beato,Guillermo P Vicent

Cellular and molecular life sciences : CMLS 81:98 PubMed38386110

2024

A progesterone derivative linked to a stable phospholipid activates breast cancer cell response without leaving the cell membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Jofre Font-Mateu,Pol Sanllehí,Jesús Sot,Beatriz Abad,Nicolas Mateos,Juan Andres Torreno-Pina,Roberto Ferrari,Roni H G Wright,Maria F Garcia-Parajo,Jesús Joglar,Félix M Goñi,Miguel Beato

International journal of cancer 136:2680-92 PubMed25363551

2014

Progestin and antiprogestin responsiveness in breast cancer is driven by the PRA/PRB ratio via AIB1 or SMRT recruitment to the CCND1 and MYC promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Wargon,Marina Riggio,Sebastián Giulianelli,Gonzalo R Sequeira,Paola Rojas,María May,María L Polo,María A Gorostiaga,Britta Jacobsen,Alfredo Molinolo,Virginia Novaro,Claudia Lanari

Cancer research 72:2416-27 PubMed22396492

2012

Estrogen receptor alpha mediates progestin-induced mammary tumor growth by interacting with progesterone receptors at the cyclin D1/MYC promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastián Giulianelli,José P Vaqué,Rocío Soldati,Victoria Wargon,Silvia I Vanzulli,Rubén Martins,Eduardo Zeitlin,Alfredo A Molinolo,Luisa A Helguero,Caroline A Lamb,J Silvio Gutkind,Claudia Lanari

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Progesterone Receptor (phospho S294) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor (phospho S294) antibody (AB61785)

Western blot - Anti-Progesterone Receptor (phospho S294) antibody (AB61785)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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