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Rabbit Recombinant Monoclonal Granulin antibody. Propeptide domain. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) (AB259664), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

The protein expressed by the GRN gene is a secreted regulator of lysosomal function and a growth factor involved in processes such as inflammation, wound healing, and cell proliferation. It regulates protein trafficking to lysosomes and lysosomal enzyme activity, facilitating lysosomal acidification which leads to the degradation of mature CTSD by CTSB. It also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation, thus preventing the release of oxidants and proteases. Additionally, it modulates inflammation in neurons by preserving neuron survival, axonal outgrowth, and neuronal integrity. Granulin-3, -4, and -7 have distinct roles: Granulin-4 promotes proliferation of epithelial cell line A431 in culture; Granulin-3 inhibits epithelial cell proliferation and induces IL-8 secretion; and Granulin-7 stabilizes CTSD by maintaining its aspartic-type peptidase activity through interaction. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN

Recommended products

Rabbit Recombinant Monoclonal Granulin antibody. Propeptide domain. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22103-42
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab259664 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Progranulin. The recommended pair for sandwich ELISA is:
Capture: Anti-Progranulin antibody [EPR22207-103] - BSA and Azide free (Capture) ab259663, Human Progranulin Capture Antibody (unconjugated)
Detector: ab259664, Human Progranulin Detector Antibody (unconjugated)
The reference range value is 156.25 - 10000 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Progranulin also known as Granulin or Proepithelin is a secreted glycoprotein with a molecular mass of approximately 68 kDa. It is expressed in a variety of tissues including the brain skin immune cells and certain epithelial cells. The protein consists of multiple cysteine-rich granulin modules which are important for its function. Progranulin plays a mechanical role in cell cycle regulation and wound healing by influencing cell proliferation and migration.

Biological function summary

This protein influences numerous cellular activities. Progranulin acts as a growth factor promoting cell survival and tissue repair. It is also a part of the lysosomal function where it helps to regulate lysosomal dynamics and turnover. It interacts with the receptor sortilin which mediates its uptake and function underlining its importance in maintaining cellular homeostasis.

Pathways

Progranulin participates in the tumor necrosis factor (TNF) pathway and the lysosome acidification pathway. It modulates TNF signaling by interacting with TNF receptors potentially balancing inflammatory responses. In the context of lysosomal pathways progranulin works closely with proteins like cathepsin D to facilitate lysosomal enzyme activity and stability ensuring effective cellular turnover and waste management.

Associated diseases and disorders

This protein has significant implications in neurodegenerative disorders and cancer. Mutations or alterations in progranulin levels associate with frontotemporal lobar degeneration (FTLD) a form of dementia. Reduced progranulin levels contribute to neuroinflammation and neuronal loss in FTLD. In cancer progranulin is linked to tumor growth and progression interacting with proteins involved in cell proliferation and survival such as transforming growth factor-beta (TGF-beta). Understanding these connections highlights its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) (ab259664), expandable thumbnail

    Sandwich ELISA - Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) (ab259664)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Human Progranulin ELISA Kit ab252364).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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