Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free
- RabMAb
- Recombinant
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(1 Publication)
Rabbit Recombinant Monoclonal Prohibitin antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
View Alternative Names
PHB, PHB1, Prohibitin 1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human liver tissue labelling Prohibitin with ab75766.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
ab75766 staining Prohibitin in the human cell line A431 (human epidermoid carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/120. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.
Isoytype control : Rabbit monoclonal IgG (Black)
Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
ab75766 staining Prohibitin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control 1 : PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human placenta tissue labelling Prohibitin with ab75766.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Overlay histogram showing HepG2 cells stained with ab75766 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75766, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Prohibitin with ab75766.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Prohibitin with ab75766 at 1/100. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IP
Unknown
Immunoprecipitation - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
ab75766 immunoprecipitating Prohibitin. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/40 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1 : HEK293 (human embryonic kidney) whole cell lysate (10ug)
Lane 2 : HEK293 (human embryonic kidney) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab75766 in HEK293 (human embryonic kidney) whole cell lysate
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
All lanes:
Immunoprecipitation - Anti-Prohibitin antibody [EP2803Y] (<a href='/en-us/products/primary-antibodies/prohibitin-antibody-ep2803y-ab75766'>ab75766</a>)
Predicted band size: 30 kDa
Observed band size: 30 kDa
false
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
ab75766 staining Prohibitin in NIH/3T3 (mouse embryonic fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab75766 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
ab75766 staining Prohibitin in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control 1 : PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
ab75766 staining Prohibitin in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control 1 : PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunocytochemistry/Immunofluorescence analysis of NIH-3T3 cells labelling Prohibitin with ab75766 at 1/100. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2803Y] - BSA and Azide free (AB239865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling Prohibitin with ab75766 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75766).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Related conjugates and formulations (2)
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Anti-Prohibitin antibody [EP2803Y]
-
578 PE
PE Anti-Prohibitin antibody [EP2803Y]
Reactivity data
Product details
ab239865 is the carrier-free version of ab75766.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein is involved in the regulation of mitochondrial respiratory function and stabilization of mitochondrial nucleoid. Prohibitin protein forms a complex with related proteins contributing to the maintenance of mitochondrial morphology and integrity. Its role extends to cell cycle control and apoptosis making it a significant player in cellular proliferation and survival. Additionally it influences cellular senescence and stress responses offering protection against various stressors.
Pathways
Prohibitin integrates into mitochondrial dynamics and energy production pathways. It participates in the regulation of oxidative phosphorylation a core component for ATP generation. Prohibitin collaborates with proteins like co-chaperones and complexes such as the inner mitochondrial membrane complex which further illustrates its integration into cellular pathways. The interplay of these proteins and pathways underlines Prohibitin's role in maintaining mitochondrial function.
Product protocols
- Visit the General protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
International archives of allergy and immunology 182:1245-1254 PubMed34428765
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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